Crops ›› 2023, Vol. 39 ›› Issue (4): 182-187.doi: 10.16035/j.issn.1001-7283.2023.04.026

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Effects of Grafting on Interspecific Hybridization Compatibility of Potato and Its Physiological Regulation Mechanism

Zhai Xinna(), Yang Jiawei, Xu Chunjiang, Qi Lipan, Tian Zaimin, Feng Yan(), Yin Jiang, Gong Xuechen   

  1. Hebei North University, Zhangjiakou 075000, Hebei, China
  • Received:2022-04-10 Revised:2022-05-30 Online:2023-08-15 Published:2023-08-15

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Abstract:

In order to cultivate excellent new germplasms of distant hybridization between tetraploid potato cultivated varieties and diploid wild varieties, tetraploid cultivated varieties (Jizhangshu 8 and Jizhangshu 12) were used as female parents and diploid wild varieties (Z1703 and Z1701) were used as male parents, the effects of pre grafting on interspecific hybridization compatibility and endogenous hormones on fruit setting rate of potato were analyzed. The results showed that the parents were pregrafted, and Jizhangshu 8 and Jizhangshu 12 were crossed with diploid wild species, respectively. After hybridization, the fruit setting rate and single fruit setting rate of each combination were improved compared with the control, the fruit setting rate of Z1703 increased by 39.19%, the number of single fruit increased by 0.13, broke through 0 seeds, and obtained the offspring of distant hybridization between cultivated and wild species. High content of GA3 and IAA were conducive to fruit setting, while high content of ABA inhibits fruit setting. Low content of ZR promotes fruit setting 2-4d after pollination, and high content of ZR promotes fruit setting in the later stage. The correlation coefficient between GA3 content and fruit setting rate was as high as 0.922 six days after pollination. The results showed that potato pre grafting could improve the fruit setting rate and promote interspecific hybridization compatibility, and endogenous hormones could regulate the fruit setting rate.

Key words: Potato, Grafting, Affinity, Physiological mechanism

Fig.1

Fruit setting of different hybrid combinations 20 days after pollination (a) G8×Z1703 control, (b) G8×Z1703 grafting; (c) G8×Z1701 control, (d) G8×Z1701 grafting"

Table 1

Effects of parental pre grafting on fruit setting rate of various inter hybrid combinations"

母本
Female
parent		 父本
Male
parent		 处理
Treatment		 授粉花朵数
Number of
pollinated flowers		 坐果数
Fruit setting
number		 坐果率
Fruit setting
rate (%)		 实生籽数
Number of
seeds		 单果结实数
Single fruit real
number
G8 Z1703 对照 191 13 6.81 0 0.00
嫁接 100 46 46.00 6 0.13
G8 Z1701 对照 188 36 19.15 12 0.33
嫁接 112 7 6.25 1 0.14
G12 Z1703 对照 265 27 10.19 9 0.33
嫁接 108 24 22.22 6 0.25
G12 Z1701 对照 160 10 6.25 2 0.20
嫁接 154 6 3.90 0 0.00

Fig.2

Content changes of endogenous hormone GA3 in different interspecific hybrid combinations After the content of the same hormone in the same hybrid combination, different letters represent significant differences (P < 0.05), the same below"

Fig.3

Content changes of endogenous hormone ZR in different interspecific hybrid combinations"

Fig.4

Content changes of endogenous hormone IAA in different interspecific hybrid combinations"

Fig.5

Content changes of endogenous hormone ABA in different interspecific hybrid combinations"

Table 2

Correlation analysis between endogenous hormone content and fruit setting rate"

相关系数
Correlation coefficient		 坐果率Fruit setting rate
GA3 ZR IAA ABA
现蕾期Budding stage 0.816* -0.705 0.457 -0.349
开花期Flowering stage -0.313 0.086 0.365 -0.302
授粉后2d Two days after pollination -0.236 0.306 -0.124 -0.199
授粉后4d Four days after pollination 0.567 -0.815* 0.660 -0.670
授粉后6d Six days after pollination 0.922** 0.477 0.487 -0.470
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