Crops ›› 2023, Vol. 39 ›› Issue (4): 65-70.doi: 10.16035/j.issn.1001-7283.2023.04.010

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Molecular Marker-Assisted Screening of Potato Germplasm Resources for Virus Resistance

Lou Shubao1,2(), Yang Mengping1, Xing Jinyue1, Zhai Lingxia1, Wang Hui1, Liu Chunsheng1, Wang Lichun1, Song Jiling1()   

  1. 1Keshan Branch of Heilongjiang Academy of Agricultural Sciences/Test-Tube Plantlet Bank of National Potato Germplasm Resources(Keshan), Qiqihar161005, Heilongjiang, China
    2Academy of Agricultural and Forestry Sciences, Qinghai University, Xining810016, Qinghai,China
  • Received:2023-03-21 Revised:2023-04-21 Online:2023-08-15 Published:2023-08-15

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Abstract:

Potato Y virus (PVY) and potato X virus (PVX) are the two most important viruses that affect the yield and quality of potato, Ryadg, Rysto and Rychc are important genes with extreme resistance to PVY, Rx is an important gene with extreme resistance to PVX. Four molecular markersclosely related to the resistance genes Ryadg, Rysto,Rychc and Rx were used to detected 102 potato varieties. The results showed that the most materials contained YES3-3B markers, accounting for 99.02% of the test materials; the least materials contained Rxsp markers, accounting for 10.78% of the test materials; four varieties contained only one marker, accounting for 3.92% of the test materials; the 62 varieties contained two markers, accounting for 60.78% of the test materials; and the 30 varieties contained three markers, accounting for 29.41%. There were six varieties containing four markers, whichwere ‘Longshu11’, ‘S.acaule’, ‘Fujin’, ‘Yunshu105’, ‘Jinshu8’ and ‘Atlantic’, accounting for 5.88% of the test material.

Key words: Potato, Virus, Molecular marker

Table 1

Information of molecular markers"

标记
Marker		 基因
Gene		 病毒
Virus		 标记类型
Marker type		 引物序列（5′-3′）
Primer sequence (5′-3′)		 片段大小
Fragment length (bp)		 温度
Temperature (℃)		 参考文献
Reference
Rxsp Rx1 PVX STS F：ATCTTGGTTTGAATACATGG
R：CACAATATTGGAAGGATTCA		 1230 58 [26]
RYSC3 Ryadg PVY SCAR F：ATACACTCATCTAAATTTGATGG
R：AGGATATACGGCATCATTTTTCCGA		 321 59 [19]
YES3-3B Rysto PVY STS F：TAACTCAAGCGGAATAACCC
R：CATGAGATTGCCTTTGGTTA		 284 55 [27]
Ry186 Rychc PVY STS F：TGGTAGGGATATTTTCCTTAGA
R：GCAAATCCTAGGTTATCAACTCA		 587 55 [28]

Fig.1

Examination of DNA concentration and quality M: DL2000 DNA marker; 1: Longshu 6; 2: Lishu 2; 3: Longshu 5; 4: Yanshu 12; 5: Yunshu 305; 6: Yanshu 1; 7: Zhongshu 4; 8: Zhongshu 16; 9: Mengshu 13; 10: Chuanyu 85; 11: Yanshu 10; 12: Lingshu 1; 13: Lishu 7; 14: Lishu 6; 15: Datongliwaihuang; 16: Hanwei 1"

Fig.2

The PCR of Rxsp marker of part of accessions M: DL2000 DNA marker; 1: Lishu 1; 2:Huasong 1; 3: Jinshu 16; 4: Huasong 88; 5: Lishu 15; 6: Ziluolan; 7: Fujin; 8: Kenjia 3; 9: Yunshu 105; 10: Beijiang 1; 11: Tongshu 29; 12: Xingjia 2; 13: Yunshu 506; 14: Huaen 1; 15: S.stenotomum; 16: Xisen 6"

Fig.3

The PCR of RYSC3 marker of part of accessions The numbered materials are the same with Fig.1"

Fig.4

The PCR of YES3-3B marker of part of accessions M: DL2000 DNA marker; 1: Tuyan 2; 2: Zhengshu 5; 3: Xingjia 3; 4: Kexin23; 5: Favorita; 6: Yunshu 202; 7: S.chacoense; 8: S.stoloniferum; 9: Dongshu 3; 10: Jinshu 8; 11: Yunshu 305; 12: Yunshu 902; 13: Yunshu 505; 14: Yunshu 107; 15: Atlantic; 16: S.pinnatisecta"

Fig.5

The PCR of Ry186 marker of part of accessions M: DL2000 DNA marker; 1: Minshu1; 2: Xinkeshu1; 3: Ningshu3; 4: Zhongshu6; 5: Yunshu401; 6: Zhongshu15; 7: Xueyu2"

Table 2

Analysis of markers of distinct varieties"

品种数量
Variety number		 类别
Class		 比例
Percentage (%)		 品种
Variety
1 只含RYSC3标记 0.98 费乌瑞它
3 只含YES3-3B标记 2.94 蒙薯13号、克新13号、扎列娃
4 同时含YES3-3B和Ry186标记 3.92 华颂88、希森6号、S.chacoense、云薯305
58









 同时含RYSC3和YES3-3B标记









 56.86









 维拉斯、陇薯6号、丽薯2号、陇薯5号、延薯1号、中薯4号、中薯16号、川芋85、延薯10号、岭薯1号、丽薯7号、丽薯6号、韩威1号、中薯2号、滇薯47、韩锦2号、晋薯15号、丽薯11号、云薯205、鄂马铃薯13号、宁蒗5号、菲勒塞纳、丽薯10号、天薯15号、泉云3号、中薯9号、冀张薯5号、华颂34、闽薯1号、鑫科薯1号、宁薯3号、中薯6号、云薯401、中薯15号、新大坪、冀张薯14号、丽薯1号、华颂1号、丽薯15号、紫罗兰、垦加3号、北疆1号、云薯506、土岩2号、郑薯5号、云薯202、陇薯10号、克新33、合作88、庄薯3号、紫花白、天薯9号、垦薯1号、黑森、黑美人、克新19号、天薯10号、Atzimba
5 同时含Rxsp、RYSC3和YES3-3B标记 4.90 大同里外黄、雪育2号、尤金、华恩1号、NS51-5
25



 同时含RYSC3、YES3-3B和Ry186标记



 24.51



 克新25号、内薯7号、克新26号、S.jamesii、延薯12号、东薯1号、华颂11、垦彩薯1号、春秋9号、北薯2号、晋薯16号、同薯29号、兴佳2号、S.stenotomum、兴佳3号、克新23号、S.stoloniferum、东薯3号、云薯902、云薯505、云薯107、S.pinnatisecta、克新34、米拉、克新1号
6 同时含Rxsp、RYSC3、YES3-3B和Ry186标记 5.88 陇薯11号、S.acaule、富金、云薯105、晋薯8号、大西洋
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