Crops ›› 2023, Vol. 39 ›› Issue (4): 253-259.doi: 10.16035/j.issn.1001-7283.2023.04.036

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Research on the Moisture Content Variation and Influence to Rice Seed Germination under Salt and Alkali Stress by Low Field NMR

Yang Hongwei1,2(), Zhang Liying3, Li Xiaohui1,2()   

  1. 1College of Information and Electrical Engineering, Shenyang Agricultural University, Shenyang 110866, Liaoning, China
    2Liaoning Engineering Research Center for Information Technology in Agriculture, Shenyang 110866, Liaoning, China
    3Liaoning Rice Research Institute, Shenyang 110161, Liaoning, China
  • Received:2022-03-06 Revised:2022-05-26 Online:2023-08-15 Published:2023-08-15

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Abstract:

In order to study the change of moisture content and its effects on the rice seeds germination under salt and alkali stress, and the feasibility of using low field nuclear magnetic detection technology to determine seed moisture content, two varieties (Yanjing 48 and Liaoxing 1) of rice seeds were placed in culture dish with the solution of 50, 150mmol/L NaCl, NaHCO3 and distilled water (control) respectively, nuclear magnetic resonance signal amplitude variation and effects of different solutions on germination characteristics were analyzed, and the regression equation was deduced between NMR signal amplitude per unit mass rice seed samples and the wet base moisture content. The results showed that the nuclear magnetic signal amplitudes of the two varieties of rice seeds increased by 65%, 95%, 115% and 135% respectively after seeds germination 6, 22, 48, and 72h compared with control treatment. After germination for the same time, the amplitude of NMR signal decreased by more than 5% under NaCl and NaHCO3 stress, the germination index decreased by more than 4.5% compared with the control treatment, indicating that there was a positive correlation between moisture content and seed germination index. There was a consistent linear correlation between NMR signal amplitude per unit mass and the wet base moisture content under different NaCl and NaHCO3 stress, R2 were all greater than 0.95. The results showed that it was reasonable and reliable to measure the water content of rice seeds under salt and alkali stress by nuclear magnetic resonance technology.

Key words: Nuclear magnetic resonance relaxation spectrum, Salt stress, Alkali stress, Rice seeds, Wet base moisture content

Table 1

Comparison of germination indexes of rice seed under NaCl and NaHCO3 stress"

水稻品种
Rice variety		 处理
Treatment		 溶液浓度
Solution concentration
(mmol/L)		 平均发芽时间
Mean germination
time (d)		 发芽指数
Germination
index		 发芽势
Germination
potential (%)		 发芽率
Germination
percentage (%)
盐粳48 YJ48 对照 0 2.05±0.05cC 24.67±0.29aA 97.33±2.31aA 100.00±0.00aA
NaCl胁迫 50 2.82±0.03bB 18.41±0.11bB 97.33±2.31aA 100.00±0.00aA
150 3.05±0.05aAB 16.22±0.49cC 86.00±2.00bB 98.67±1.15aA
NaHCO3胁迫 50 2.99±0.08abAB 16.02±0.40cC 95.83±3.82aA 97.50±3.00abA
150 3.19±0.19aA 14.28±0.40dD 88.33±1.44bB 94.17±4.00bA
辽星1 LX1 对照 0 2.50±0.04cC 20.89±0.18aA 90.48±2.36aA 98.00±0.00aA
NaCl胁迫 50 3.05±0.04bB 16.37±0.37bB 92.56±1.26aA 98.00±2.00aA
150 4.41±0.10aA 10.03±0.28dD 88.26±1.37cC 93.33±4.16bB
NaHCO3胁迫 50 3.38±0.05bB 14.28±0.21cC 92.50.±2.50aA 96.67±1.00aA
150 4.53±0.07aA 9.82±0.17dD 38.33.±1.44bB 88.33±1.00cC

Fig.1

T2 inversion spectrum of per unit mass YJ48 seed s under NaCl stress"

Fig.2

T2 inversion spectrum of per unit mass YJ48 seeds under NaHCO3 stress"

Table 2

NMR signal amplitude (A21 and A22) per unit mass YJ48 under NaCl and NaHCO3 stress"

溶液
Solution		 萌发时间
Germination time (h)		 对照CK 50mmol/L 150mmol/L
A21 A22 A21 A22 A21 A22
NaCl 初始 4497.0±58.6eE 408.6±8.3eE 4497.0±58.6eE 408.6±8.3eE 4497.0±58.6eE 408.6±8.3eE
6 7594.0±103.3dD 842.2±37.9dD 7331.2±95.4dD 829.5±31.9dD 7289.6±76.2dD 854.3±45.2dD
24 8874.6±102.3cC 1052.7±29.8cC 8868.8±73.3cC 1026.0±46.4cC 8809.8±90.6cC 1028.5±51.5cC
48 9248.3±132.1bB 1806.6±53.3bB 9319.0±73.9bB 1725.2±45.8bB 9510.3±73.5bB 1180.3±82.4bB
72 9059.4±104.5aA 3023.0±151.1aA 9458.0±122.0aA 2523.1±132.5aA 9055.4±128.2aA 1979.9±80.1aA
NaHCO3 初始 4497.0±58.6eE 408.6±8.3eE 4497.0±58.6eE 408.6±8.3eE 4497.0±58.6eE 408.6±8.3eE
6 7594.0±103.3dD 842.2±37.9dD 7394.4±85.6dD 846.2±42.1dD 7365.4±68.5dD 862.3±44.8dD
24 8874.6±102.3cC 1052.7±29.8cC 8885.4±71.6cC 1039.9±43.2cC 8829.7±85.3cC 1040.3±50.2cC
48 9248.3±132.1bB 1806.6±53.3bB 9307.1±72.8bB 1745.1±46.3bB 9103.2±65.3bB 1649.3±78.7bB
72 9059.4±104.5aA 3023.0±151.1aA 9487.8±118.6aA 2568.3±127.5aA 9369.9±116.7aA 2082.8±76.8aA

Table 3

NMR signal amplitude (A21 and A22) per unit mass LX1 under NaCl and NaHCO3 stress"

溶液
Solution		 萌发时间
Germination time (h)		 对照CK 50mmol/L 150mmol/L
A21 A22 A21 A22 A21 A22
NaCl 初始 4381.1±49.0eE 267.4±4.0eE 4381.1±49.0eE 267.4±4.0eE 4381.1±49.0eE 267.4±4.0eE
6 6890.9±86.5dD 708.3±32.8dD 6774.1±41.6dD 733.5±38.8dD 6733.5±107.6dD 743.2±22.4dD
24 8062.9±68.6cC 806.4±50.7cC 8011.5±130.8cC 827.7±32.0cC 8013.9±93.7cC 824.5±27.0cC
48 8970.2±80.2bB 1252.5±48.7bB 8668.3±103.9bB 1217.0±46.6bB 8756.7±138.2bB 983.0±25.6bB
72 8603.4±116.1aA 2050.5±94.8aA 8920.2±134.8aA 1682.4±88.2aA 8918.4±51.3aA 1265.3±56.1aA
NaHCO3 初始 4381.1±49.0eE 267.4±4.0eE 4381.1±49.0eE 267.4±4.0eE 4381.1±49.0eE 267.4±4.0eE
6 6890.9±86.5dD 708.3±32.8dD 6775.1±40.8dD 736.5±36.7dD 6747.5±98.6dD 746.2±20.8dD
24 8062.9±68.6cC 806.4±50.7cC 8024.2±105.2cC 829.7±37.6cC 8021.5±89.2cC 828.0±22.5cC
48 8970.2±80.2bB 1252.5±48.7bB 8717.7±98.2bB 1226.3±42.8bB 8790.4±125.6bB 1011.9±21.2bB
72 8603.4±116.1aA 2050.5±94.8aA 8923.7±102.7aA 1701.3±75.9aA 8947.9±48.2aA 1274.5±20.9aA

Fig.3

Comparison of NMR signal amplitude of per unit mass YJ48 seeds after germination 6, 24, 48, 72h under NaCl and NaHCO3 stress The different capital and lowercase letters indicate significant difference at 0.01 and 0.05 levels, respectively"

Fig.4

Regression between MC and NMR signal amplitude of per unit mass YJ48 seeds under NaCl stress"

Fig.5

Regression between MC and NMR signal amplitude of per unit mass YJ48 seeds under NaHCO3 stress"

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