Crops ›› 2017, Vol. 33 ›› Issue (3): 151-156.doi: 10.16035/j.issn.1001-7283.2017.03.027

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Analysis of Genetic Diversity of Alternaria solani Isolated from Potato in Heilongjiang Province

Tai Lianmei,Zuo Yuhu,Zhang Yaling,Jin Yongling,Guo Yongxia,Jin Xuehui,Jin Guanghui   

  1. College of Agronomy,Heilongjiang Bayi Agricultural University,Daqing 163319,Heilongjiang,China
  • Received:2017-01-09 Revised:2017-03-25 Online:2017-06-15 Published:2018-08-26

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Abstract:

To explore genetic diversity of Alternaria solani infesting potato in Heilongjiang, 67 isolates of A.solani were collected from different localities and the genetic diversity of these isolates was analyzed with RAPD and ISSR markers in our study. The results showed that 53 fragments were amplified with 5 RAPD primers, 75.5% of which were polymorphic, and 93 fragments were amplified with 10 ISSR primers, 88.2% of which were polymorphic. Tested isolates of A.solani were divided into different groups with RAPD and ISSR markers according to cluster analysis, and indicated that these isolates had abundant heredity differentiation. Both RAPD and ISSR markers could reveal the genetic diversity of A.solani isolates, whereas there was no obvious relationship between geographic regions of isolates and different groups. These results provided the theory evidence for breeding varieties for disease resistance and control potato early blight in Heilongjiang in future.

Key words: Potato early blight, Alternaria solani, RAPD, ISSR, Genetic diversity

Table 1

Strain codes and collection sites"

菌株编号Strain code 采集地点Collection site 菌株编号Strain code 采集地点Collection site
SH0801~SH0806 绥化 NH0801~NH0810 讷河
HL0801~HL0808 海伦 HS0801~HS0803 鹤山农场
JG0801~JG0808 加格达奇 JS0801~JS0802 九三管分局
KS0801~KS0814,KS0817~KS0823 克山 NJ0801~NJ0804 嫩江农场
DQ0801~DQ0804 大庆 AD0701 安达

Table 2

The band numbers of amplification with RAPD and ISSR primers"

RAPD ISSR
引物
Primer		 扩增条带数
Amplified band
number		 多态性条带数
Polymorphism
band number		 多态性片段比率(%)
Proportion of
polymorphic fragments		 引物
Primer		 扩增条带数
Amplified band
number		 多态性条带数
Polymorphism
band number		 多态性片段比率(%)
Proportion of
polymorphic fragments
203 19 7 77.7 807 19 18 88.8
248 18 8 100.0 811 13 12 92.3
284 12 7 58.3 826 17 16 85.7
285 13 8 61.5 836 16 15 88.3
295 11 10 90.9 841 11 10 90.9
856 18 16 75.0
864 11 10 90.9
887 14 13 75.0
888 14 12 85.7
890 10 10 100.0
合计 53 40 - 合计 93 82 -
平均 10.6 8.0 75.5 平均 19.3 18.2 88.2

Fig.1

Pattern of RAPD amplification with primer 295"

Fig.2

Pattern of ISSR amplification with primer 841"

Fig.3

Genetic dendrogram of 67 strains of .solani using RAPD marker"

Fig.4

Genetic dendrogram of 67 strains of .solani using ISSR marker"

[1] 台莲梅, 郑寰宇, 左豫虎 , 等. 黑龙江省马铃薯早疫病菌生物学特性研究. 植物保护, 2012,38(1):85-89.
doi: 10.3969/j.issn.0529-1542.2012.01.017
[2] 郑寰宇, 马力, 左豫虎 , 等. 马铃薯早疫病菌分生孢子萌发条件的研究. 植物保护, 2010,36(6):91-95.
doi: 10.3969/j.issn.0529-1542.2010.06.020
[3] 刘丽丽, 朱杰华, 崔亚靖 , 等. 培养条件对茄链格孢产孢的影响. 菌物学报, 2014,33(3):659-667.
doi: 10.13346/j.mycosystema.130038
[4] Shahbazi H, Aminian H, Sahebani N , et al. Biochemical evaluation of resistance responses of potato to different isolates of Alteraria solani. Phytopathology, 2010,100(5):454-459.
doi: 10.1094/PHYTO-100-5-0454
[5] 台莲梅, 梁伟伶, 左豫虎 , 等. 马铃薯不同品种感染早疫病菌后防御酶活性变化. 植物生理学通讯, 2010,46(11):1147-1150.
[6] 左豫虎, 史洁, 韩青梅 , 等. 茄链格孢菌侵染马铃薯叶片过程的细胞学观察.中国蔬菜, 2011(14):35-40.
[7] 梁伟玲, 台莲梅, 靳学慧 , 等. 马铃薯早疫病菌室内杀菌剂筛选及配比试验. 植物保护, 2009,35(4):168-171.
doi: 10.3969/j.issn.0529-1542.2009.04.041
[8] 姚亮亮, 张铉哲, 赵宇 . 黑龙江省分离的马铃薯早疫病病菌对腐霉利和戊唑醇的敏感性分析.作物杂志, 2011(3):29-33.
doi: 10.3969/j.issn.1001-7283.2011.03.007
[9] Weir T L, Huff D R, Christ B J , et al. RAPD-PCR analysis of genetic variation among isolates of Alternaria solani and Alternaria alternata from potato and tomato. Mycologia, 1998,90(5):813-821.
doi: 10.1080/00275514.1998.12026975
[10] Kumar V, Haldar S, Pandey K K , et al. Cultural morphological,pathogenic and molecular variability amongst tomato isolates of Alternaria solani in India. World Journal of Microbiology and Biotechnology, 2008,24:1003-1009.
doi: 10.1007/s11274-007-9568-3
[11] Martínez S P, Snowdon R, Ponskühnemann J . Variability of cuban and international populations of Alternaria solani from different hosts and localities:AFLP genetic analysis. European Journal of Plant Pathology, 2004,110:399-409.
doi: 10.1023/B:EJPP.0000021071.65146.c0
[12] 张福光, 杨志辉, 朱杰华 , 等. 河北省马铃薯早疫病菌群体遗传结构的研究. 菌物学报, 2012,31(1):40-49.
[13] Zietkiewicz E, Rafalski A, Labuda D . Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification. Genomics, 1994,20:176-183.
doi: 10.1006/geno.1994.1151
[14] 周延清, 景建洲, 李振勇 , 等. 利用RAPD和ISSR分子标记分析地黄种质遗传多样性. 遗传, 2004,26(6):922-928.
[15] 臧睿, 黄丽丽, 李正力 , 等. 陕西省苹果树腐烂病菌基因多态性的ISSR分析. 植物保护学报, 2012,39(1):51-57.
[16] 胡中会, 赵立华, 孔宝华 , 等. 烟草赤星病菌遗传多样性的ISSR和RAPD标记比较分析. 西南农业学报, 2012,25(3):917-921.
doi: 10.3969/j.issn.1001-4829.2012.03.036
[17] 陈亮, 梅明华, 徐才国 , 等. RFLP、RAPD、AFLP在水稻农垦58S和1514中多态性比较. 植物学报, 2000,17(5):424-428.
[18] 陈瑞辉, 周晓云, 王克荣 . 大丽轮枝菌RAPD反应组分的优化与应用. 农业生物技术学报, 2002,10(3):291-294.
[19] 王健生, 李潇, 张海峰 , 等. 大豆枯萎病菌尖孢镰孢遗传多样性及大豆品种抗性. 植物病理学报, 2015,95(2):167-179.
doi: 10.13926/j.cnki.apps.2015.02.007
[20] 周延清, 景建洲, 李振勇 , 等. 利用RAPD和ISSR分子标记分析地黄种质遗传多样性. 遗传, 2004,26(6):922-928.
[21] van der Waals J E, Korsten J, Slippers B . Genetic diversity among Alternaria solani isolates from potatoes in South Africa. Plant Disease, 2004,88(9):959-964.
doi: 10.1094/PDIS.2004.88.9.959
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