Crops ›› 2022, Vol. 38 ›› Issue (1): 213-219.doi: 10.16035/j.issn.1001-7283.2022.01.032

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Identification of Salt Tolerance during Germination of Maize Inbred Lines

Duan Yajuan1,2(), Cao Shiliang2, Yu Tao2, Li Wenyue2, Yang Gengbin2, Wang Chengbo2, Liu Baomin2, Liu Changhua1()   

  1. 1College of Advanced Agriculture and Ecological Environment, Heilongjiang University, Harbin 150080, Heilongjiang, China
    2Maize Research Institute, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, Heilongjiang, China
  • Received:2021-08-18 Revised:2021-12-08 Online:2022-02-15 Published:2022-02-16
  • Contact: Liu Changhua E-mail:Duanyajuan2902@163.com;liuchanghua70@163.com

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Abstract:

In order to optimize the evaluation criteria for salt tolerance of maize inbred lines during germination period, we selected maize varieties with strong salt tolerance, analyzed the genetic mechanism of salt tolerance in maize inbred lines during germination period, which provide important theoretical basis and technical support for salt tolerance breeding in maize germination period. The ten physiological indexes related to salt tolerance of 390 maize inbred lines were measured, and five independent comprehensive indexes (the 7-day germination rate, the 7-day bud dry weight, the 7-day bud fresh weight, the 7-day root dry weight and root length) were determined by principal component analysis. The five comprehensive indexes can be used as the identification indicators of salt tolerance during the germination period of maize. According to the membership function analysis, the comprehensive salt tolerance index of each material was determined, and on this basis, through cluster analysis, the test materials were divided into six grades, according to salt-tolerance during germination period, including 298 high salt-sensitive inbred lines, 49 moderate salt-sensitive inbred lines, 27 salt-sensitive inbred lines, nine salt-tolerant inbred lines, five moderate salt-tolerant inbred lines, and two high salt-tolerant inbred ines.

Key words: Maize, Germination period, Salt tolerance, Principal component analysis, Cluster analysis

Table 1

Statistical analysis of salt tolerance indexes of maize inbred lines"

发芽天数
Germination days (d)		 耐盐指标
Salt-tolerance index		 平均值
Average		 最小值
Minimum		 最大值
Maximum		 标准差
Standard deviation		 方差
Variance		 变异系数
Coefficient of variation (%)
4 生根率Rooting rate (%) 31.9100 0 100.0000 22.7300 5.1800 71.25
发芽率Germination rate (%) 8.0500 0 93.3300 13.6900 1.8800 170.04
7 生根率Rooting rate (%) 40.6500 0 100.0000 24.6100 6.0700 60.55
发芽率Germination rate (%) 32.4400 0 100.0000 22.4500 5.0500 69.21
根鲜重Root fresh weight (g) 0.0496 0 0.2337 0.0327 0.0011 0.66
芽鲜重Bud fresh weight (g) 0.0378 0 0.2540 0.0322 0.0010 0.85
根干重Root dry weight (g) 0.0056 0 0.0229 0.0037 0.0000 0.65
芽干重Bud dry weight (g) 0.0051 0 0.0279 0.0038 0.0000 0.75
根长Root length (cm) 3.5497 0 6.3800 1.3364 1.7906 0.38
芽长Bud length (cm) 1.3746 0 3.3400 0.7800 0.6100 0.57

Table 2

Correlation analysis between salt tolerance indexes of maize inbred lines"

发芽天数
Germination
days (d)		 耐盐指标
Salt-tolerance index		 4d 7d 根长
Root
length		 芽长
Bud
length
生根率
Rooting
rate		 发芽率
Germination
rate		 生根率
Rooting
rate		 发芽率
Germination
rate		 根鲜重
Root fresh
weight		 芽鲜重
Bud fresh
weight		 根干重
Root dry
weight		 芽干重
Bud dry
weight
4 生根率Rooting rate 1 0.61** 0.93** 0.90** 0.49** 0.46** 0.54** 0.58** 0.41** 0.56**
发芽率Germination rate – 1 0.57** 0.64** 0.46** 0.55** 0.47** 0.61** 0.31** 0.58**
7 生根率Rooting rate – – 1 0.94** 0.52** 0.42** 0.58** 0.56** 0.42** 0.54**
发芽率Germination rate – – – 1 0.55** 0.51** 0.59** 0.64** 0.45** 0.63**
根鲜重Root fresh weight – – – – 1 0.65** 0.84** 0.67** 0.59** 0.51**
芽鲜重Bud fresh weight – – – – – 1 0.56** 0.86** 0.49** 0.73**
根干重Root dry weight – – – – – – 1 0.72** 0.55** 0.52**
芽干重Bud dry weight – – – – – – – 1 0.52** 0.76**
根长Root length – – – – – – – – 1 0.62**
芽长Bud length – – – – – – – – – 1

Table 3

The feature vector and contribution rate of the principal components of each index"

发芽天数
Germination days (d)		 耐盐指标
Salt-tolerance index		 主成分Principal component
1 2 3 4
4 生根率Rooting rate 0.3273 -0.4496 0.0475 0.0877
发芽率Germination rate 0.2902 -0.1506 -0.4142 -0.2778
7 生根率Rooting rate 0.3276 -0.4562 0.1548 0.0816
发芽率Germination rate 0.3464 -0.3844 0.0268 0.0722
根鲜重Root fresh weight 0.3136 0.2740 0.4463 -0.2577
芽鲜重Bud fresh weight 0.3118 0.3477 -0.3527 -0.1782
根干重Root dry weight 0.3192 0.1837 0.4655 -0.3042
芽干重Bud dry weight 0.3456 0.2287 -0.2179 -0.2221
根长Root length 0.2602 0.3198 0.2442 0.7044
芽长Bud length 0.3110 0.1873 -0.3926 0.4088
特征值Eigenvalue 6.2840 1.2493 0.7666 0.6537
贡献率Contribution rate (%) 62.8403 12.4934 7.6659 6.5368
累计贡献率Cumulative contribution rate (%) 62.8403 75.3337 82.9996 89.5364

Table 4

D values and comprehensive evaluation of some maize inbred lines"

编号Number 材料名称Material name U(X1) U(X2) U(X3) U(X4) D值D value 耐盐等级Salt-tolerance grade
290 18GWASPHG356 1.00 0.97 0.94 1.00 0.99 高度耐盐型
186 18GWASPHG228 0.86 1.00 0.74 0.84 0.86 高度耐盐型
160 18GWASPHG196 0.68 0.77 0.75 0.55 0.69 中度耐盐型
161 18GWASPHG198 0.68 0.83 0.33 0.61 0.66 中度耐盐型
336 18GWASPHG416 0.71 0.83 0.15 0.48 0.66 中度耐盐型
248 18GWASPHG305 0.71 0.67 0.22 0.30 0.63 中度耐盐型
171 18GWASPHG208 0.61 0.93 0.31 0.41 0.61 中度耐盐型
261 18GWASPHG319 0.64 0.83 0.11 0.21 0.58 耐盐型
22 18GWASPHG024 0.57 0.63 0.36 0.36 0.55 耐盐型
142 18GWASPHG176 0.57 0.63 0.31 0.17 0.53 耐盐型
80 18GWASPHG098 0.50 0.83 0.32 0.31 0.52 耐盐型
164 18GWASPHG201 0.54 0.70 0.14 0.37 0.52 耐盐型
245 18GWASPHG302 0.50 0.77 0.17 0.39 0.51 耐盐型
313 18GWASPHG390 0.50 0.77 0.26 0.25 0.49 耐盐型
33 18GWASPHG039 0.39 0.77 0.26 0.69 0.46 耐盐型
325 18GWASPHG403 0.46 0.5 0.40 0.41 0.46 耐盐型
121 18GWASPHG149 0.39 0.43 0.59 0.55 0.43 盐敏感型
367 18GWASPHG451 0.43 0.50 0.29 0.33 0.43 盐敏感型
52 18GWASPHG066 0.39 0.63 0.30 0.36 0.41 盐敏感型
70 18GWASPHG086 0.39 0.60 0.24 0.39 0.41 盐敏感型
289 18GWASPHG355 0.36 0.80 0.21 0.29 0.41 盐敏感型
242 18GWASPHG299 0.39 0.53 0.16 0.41 0.40 盐敏感型
255 18GWASPHG312 0.39 0.47 0.21 0.37 0.39 盐敏感型
360 18GWASPHG443 0.32 0.73 0.26 0.41 0.39 盐敏感型
389 18GWASPHG478 0.36 0.5 0.27 0.55 0.39 盐敏感型
98 18GWASPHG119 0.32 0.73 0.21 0.45 0.38 盐敏感型
173 18GWASPHG210 0.29 0.73 0.48 0.45 0.38 盐敏感型
210 18GWASPHG258 0.32 0.77 0.23 0.34 0.38 盐敏感型
213 18GWASPHG261 0.39 0.60 0.24 0.15 0.38 盐敏感型
93 18GWASPHG113 0.32 0.60 0.21 0.50 0.37 盐敏感型
60 18GWASPHG074 0.36 0.57 0.21 0.18 0.36 盐敏感型
73 18GWASPHG090 0.29 0.77 0.14 0.51 0.36 盐敏感型
111 18GWASPHG134 0.32 0.60 0.20 0.28 0.35 盐敏感型
149 18GWASPHG183 0.32 0.63 0.17 0.34 0.35 盐敏感型
345 18GWASPHG428 0.29 0.50 0.51 0.40 0.35 盐敏感型
368 18GWASPHG452 0.29 0.80 0.13 0.36 0.35 盐敏感型
29 18GWASPHG032 0.29 0.57 0.26 0.48 0.34 盐敏感型
88 18GWASPHG107 0.29 0.40 0.45 0.46 0.34 盐敏感型
191 18GWASPHG234 0.25 0.80 0.21 0.36 0.34 盐敏感型
86 18GWASPHG105 0.25 0.43 0.45 0.37 0.32 盐敏感型
94 18GWASPHG114 0.25 0.63 0.25 0.33 0.32 盐敏感型
167 18GWASPHG204 0.25 0.63 0.17 0.49 0.32 盐敏感型
319 18GWASPHG397 0.21 0.60 0.52 0.41 0.32 盐敏感型

Fig.1

Cluster map of salt tolerance of 390 maize inbred lines at germination stage"

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