Crops ›› 2018, Vol. 34 ›› Issue (1): 166-172.doi: 10.16035/j.issn.1001-7283.2018.01.027

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Effects of Seed Soaking with 6-BA on Germination and Physiological Characteristics of Mung Bean under Salt Stress

Liu Jianxia1,Zhang Xiaodan1,Wang Runmei1,Zhou Feng1,Liu Wenying1,Liu Zhiping2   

  1. 1 College of Life Science, Datong University, Datong 037009, Shanxi, China
    2 High Latitude Crops Institute,Shanxi Academy of Agricultural Sciences, Datong 037008, Shanxi, China
  • Received:2017-06-30 Revised:2017-10-08 Online:2018-02-20 Published:2018-08-24

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Abstract:

6-benzylaminoadenine (6-BA) is a synthetic cytokinin that promotes seed germination, but slows down flower and fruit aging. The effects of 6-BA on seed germination and physiological and biochemical indexes of mung bean were studied by seed soaking with different concentrations of 6-BA. The results showed that: (1) Low concentration of 6-BA (≤10mg/L) could alleviate the effect of salt stress on seed germination and increase the germination potential and germination rate of mung bean seed. When the soaking concentration of 6-BA (≥20mg/L)was increased, seed germination was inhibited. (2) Low concentration of salt stress will promote the elongation of hypocotyls of roots and seedlings, and the high concentration of salt stress inhibited root growth. 6-BA pretreatment inhibited the elongation of hypocotyls of roots and seedlings, and the higher the concentration of 6-BA, the stronger the inhibition. (3) Compared with the control, salt stress could significantly increase the content of proline (Pro) in mung bean seedlings and cause the increase of malondialdehyde (MDA). but decreased of peroxidase (POD) activity. After pretreatment with low concentration of 6-BA, the contents of Pro and MDA were inhibited under salt stress treatment. Compared with salt stress alone, after high concentration of 6-BA pretreatment, SOD and POD activity of salt stress treatment were increased. The results showed that the seed soaking with suitable concentration 6-BA could promote the germination and seedling growth of mung bean under salt stress.

Key words: Mung bean, 6-BA soaking, Salt stress, Germination, Seedling physiological characteristics

Fig.1

Effects of seed soaking with 6-BA on germination index of mung bean seeds under NaCl stress Different letters in the figure indicate the difference in the treatment of 6-BA at different concentrations under the same salt stress is significant at 0.05 level, the same below"

Fig.2

Effects of seed soaking with 6-BA on Pro content in mung bean seedlings under salt stress"

Fig.3

Effects of seed soaking with 6-BA on MDA content in nung bean seedlings under salt stress"

Fig.4

Effects of seed soaking with 6-BA on SOD activity in mung bean seedlings under salt stress"

Fig.5

Effects of seed soaking with 6-BA on POD activity in mung bean seedlings under salt stress"

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