Crops ›› 2016, Vol. 32 ›› Issue (3): 27-32.doi: 10.16035/j.issn.1001-7283.2016.03.006

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Type Identification of Cytoplasmic Male Sterile Line and Study of Pollen Abortion in Maize

Sun Lifang,Deng Jie,Wang Xia,Zhao Wei,Yang Kejun,Miao Xingfen,Gao Shuren   

  1. College of Agronomy,Heilongjiang Bayi Agricultural University,Daqing163319,Heilongjiang,China
  • Received:2016-01-22 Revised:2016-04-22 Online:2016-06-15 Published:2018-08-26

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Abstract:

In this study, the fertility investigation in the field, the testing of pollen viability, PCR amplification of cytoplasmic male sterility with specific primers, identification of maydis resistance and paraffin were used to identify the type of cytoplasmic male sterility after chemical mutagenesis. The results showed that the sterile line 85218A was completely aborted in field. It was a typical yellowish round abortion after I2-KI staining, and was a C-type CMS.It showed mid-tolerance to maydis race C. The critical period of pollen abortion was late mononuclear. This study broadened the male sterility of maize germplasm resources.

Key words: Maize, Cytoplasmic, Male sterile line, Cytoplasmic type, Pollen abortion, Identification

Table 1

Compare the anther size of CMS and maintainer line mm"

自交系
Inbred line		 长度
Length		 横截面直径
Cross-sectional diameter
85218A 4.57±0.12c 0.78±0.12b
85218B 5.77±0.11a 1.19±0.06a

Fig.1

Identification of sterile cytoplasm by PCR using cytoplasmic type specifically primers A.Genome DNA; B.Identification the type of sterile cytoplasm by PCR; M.2000bp Marker; 1.He344; 2.DT-He344; 3.ZT-He344; 4.T103; 5.HD103; 6.85218A; 7.85218B"

Table 2

Reaction of Bipolaris maydis between the CMS and maintainer line"

自交系
Inbred line		 平均级别（抗性）Average level(resistance)
灭多威Methomyl C小种C race CK
85218A 2（R） 4（MR） 1（R）
85218B 1（R） 1（R） 1（R）

Fig.2

Microspore growth situation of maintainer 85218B A.The stage of pollen mother cells(400×);B.Free pollen mother cells(400×);C.The dyad period(400×);D.Free tetrad cells(100×);E.The mononuclear period(400×);F.Free mononuclear mid microspores(400×);G.The binucleate period(400×);H.Three nuclear spores(400×);I.The sachet of fully grown spores(100×);J.Mature pollens(100×)"

Fig.3

Microspore growth situation of CMS 85218A A.The stage of pollen mother cells(400×); B.Free pollen mother cells(400×); C.The dyad period(400×); D.Free dyad cells(400×); E.The tetrad period(400×); F.Free tetrad cells(400×); G.Free mononuclear early microspores(400×); H.The mononuclear period(400×); I.Monocytes late period(100×); J.The binucleate period(400×); K.Dual-core spores(400×); L.The sachet of grown spores fully(100×); M.Pollen fragments after abortion(400×)"

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