Crops ›› 2017, Vol. 33 ›› Issue (3): 151-156.doi: 10.16035/j.issn.1001-7283.2017.03.027

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Analysis of Genetic Diversity of Alternaria solani Isolated from Potato in Heilongjiang Province

Tai Lianmei,Zuo Yuhu,Zhang Yaling,Jin Yongling,Guo Yongxia,Jin Xuehui,Jin Guanghui   

  1. College of Agronomy,Heilongjiang Bayi Agricultural University,Daqing 163319,Heilongjiang,China
  • Received:2017-01-09 Revised:2017-03-25 Online:2017-06-15 Published:2018-08-26

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Abstract:

To explore genetic diversity of Alternaria solani infesting potato in Heilongjiang, 67 isolates of A.solani were collected from different localities and the genetic diversity of these isolates was analyzed with RAPD and ISSR markers in our study. The results showed that 53 fragments were amplified with 5 RAPD primers, 75.5% of which were polymorphic, and 93 fragments were amplified with 10 ISSR primers, 88.2% of which were polymorphic. Tested isolates of A.solani were divided into different groups with RAPD and ISSR markers according to cluster analysis, and indicated that these isolates had abundant heredity differentiation. Both RAPD and ISSR markers could reveal the genetic diversity of A.solani isolates, whereas there was no obvious relationship between geographic regions of isolates and different groups. These results provided the theory evidence for breeding varieties for disease resistance and control potato early blight in Heilongjiang in future.

Key words: Potato early blight, Alternaria solani, RAPD, ISSR, Genetic diversity

Table 1

Strain codes and collection sites"

菌株编号Strain code 采集地点Collection site 菌株编号Strain code 采集地点Collection site
SH0801~SH0806 绥化 NH0801~NH0810 讷河
HL0801~HL0808 海伦 HS0801~HS0803 鹤山农场
JG0801~JG0808 加格达奇 JS0801~JS0802 九三管分局
KS0801~KS0814,KS0817~KS0823 克山 NJ0801~NJ0804 嫩江农场
DQ0801~DQ0804 大庆 AD0701 安达

Table 2

The band numbers of amplification with RAPD and ISSR primers"

RAPD ISSR
引物
Primer		 扩增条带数
Amplified band
number		 多态性条带数
Polymorphism
band number		 多态性片段比率(%)
Proportion of
polymorphic fragments		 引物
Primer		 扩增条带数
Amplified band
number		 多态性条带数
Polymorphism
band number		 多态性片段比率(%)
Proportion of
polymorphic fragments
203 19 7 77.7 807 19 18 88.8
248 18 8 100.0 811 13 12 92.3
284 12 7 58.3 826 17 16 85.7
285 13 8 61.5 836 16 15 88.3
295 11 10 90.9 841 11 10 90.9
856 18 16 75.0
864 11 10 90.9
887 14 13 75.0
888 14 12 85.7
890 10 10 100.0
合计 53 40 - 合计 93 82 -
平均 10.6 8.0 75.5 平均 19.3 18.2 88.2

Fig.1

Pattern of RAPD amplification with primer 295"

Fig.2

Pattern of ISSR amplification with primer 841"

Fig.3

Genetic dendrogram of 67 strains of .solani using RAPD marker"

Fig.4

Genetic dendrogram of 67 strains of .solani using ISSR marker"

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