Crops ›› 2019, Vol. 35 ›› Issue (6): 33-42.doi: 10.16035/j.issn.1001-7283.2019.06.006

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Identification and Expression Analysis of ASR Family Genes in Setaria italica

Song Jian1,2,Cao Xiaoning3,Wang Haigang3,Chen Ling3,Wang Junjie3,Liu Sichen3,Qiao Zhijun3   

  1. 1College of Biological Engineering, Shanxi University, Taiyuan 030006, Shanxi, China
    2Institute of Industrial Crop, Shanxi Academy of Agricultural Sciences, Fenyang 032200, Shanxi, China
    3Institute of Crop Germplasm Resources, Shanxi Academy of Agricultural Sciences, Taiyuan 030031, Shanxi, China
  • Received:2019-06-05 Revised:2019-10-11 Online:2019-12-15 Published:2019-12-11
  • Contact: Sichen Liu,Zhijun Qiao

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Abstract:

ASR protein is a plant-specific protein, which it is one of the most important proteins involved in the drought resistance and salt tolerance of plants. Bioinformatics methods were used to analyze the physicochemical properties of proteins, promoter analysis and phylogenetic tree construction of eight SiASRs family genes in millet. PEG-6000 (10%) and salt (150mmol/L) stresses were arranged for study expression using qRT-PCR. The proteins encoded by the 8 millet SiASRs family genes contained typical ABA/WDS structures, and no signal peptides. Phylogenetic tree showed that the genetic relationship between millet and switchgrass was closer. The evolution among millet ASR protein and tomato ASR protein and soybean ASR protein was relatively far. The results of expression analysis under PEG stress showed that the expression of 8 SiASR was significantly different in different tissues after induction by drought stress. The results of expression analysis under salt stress showed that the responses of 8 ASR genes to salt stress were mainly reflected in the leaves, and the overall expression was up-regulated. The expression of 8 ASR genes in stems was stable; In the roots, ASR1, ASR2, ASR4, ASR5 and ASR6 were involved in salt stress response, and all 5 genes were up-regulated.

Key words: Setaria italica, ASR, Family gene, Bioinformatics, Expression analysis

Fig.1

SiASR family genes chromosome mapping"

Table 1

Members of SiASR family genes and their physicochemical properties"

基因名称
Gene name		 基因ID
Gene ID		 基因长度(bp)
Length of gene		 编码区(bp)Length of CDS 氨基酸数目(aa)
Number of amino acids		 基因所在染色体的位置
Location of the genes on the chromosome		 等电点
PI		 分子量(kDa)
Molecular weight		 亲水性指数
Hydrophilic index
SiASR1 Seita.1G187200 876 324 107 scaffold_1：26937730..26938605 reverse 6.76 11.82 -1.190
SiASR2 Seita.5G463600 1 020 522 173 scaffold_5：46902215..46903234 forward 6.26 19.38 -1.277
SiASR3 Seita.5G463700 1 167 318 105 scaffold_5：46906469..46907635 reverse 9.73 11.71 -1.147
SiASR4 Seita.5G463800 590 309 103 scaffold_5：46907284..46907873 forward 9.80 11.50 -1.417
SiASR5 Seita.7G097100 803 306 101 scaffold_7：19994321..19995123 reverse 6.81 11.48 -1.366
SiASR6 Seita.7G097200 1 318 735 244 scaffold_7：19998219..19999536 reverse 5.03 26.00 -1.636
SiASR7 Seita.7G291500 984 417 138 scaffold_7：33499331..33500314 forward 5.88 15.45 -1.298
SiASR8 Seita.8G045700 917 414 137 scaffold_8：3645891..3646807 reverse 6.15 15.42 -1.346

Fig.2

Phylogenetic analysis of ASRs protein"

Fig.3

SiASRs protein phylogenetic tree, SiASRs gene structure and motif distribution of SiASRs protein A, The phylogenetic tree of SiASRs protein; B, SiASRs gene structure map; C, The conserved motifs of SiASRs protein"

Table 2

Motifs of SiASRs proteins"

基序名称
Motif name		 基序宽度
Motif width		 基序的氨基酸组成
Amino acid composition of the motif
motif1 50 QLGELGAVAAGAYALYEKHKAKKDPEHAHRHKIKEEVAAAAAVGSGGYAF
motif2 14 EDYKKEEKEHKHKE
motif3 11 HEHHEKKEAKK
motif4 18 MAEEKHHHHYFHHHKDED
motif5 6 QQPAGG
motif6 6 SGTDEC
motif7 8 TVAEEVVT
motif8 6 DDCYNG
motif9 8 RVGAGGYC
motif10 6 KHHHLF
motif11 6 HGSRRD
motif12 7 VGRRGGG
motif13 11 RNRAVGDDEYN
motif14 6 KKHHFF
motif15 6 DDEKNK
motif16 6 YGGGYN
motif17 7 YGRGGGD
motif18 6 HRRHGH

Fig.4

Relative expression of SiASR family genes in roots, stems and leaves before stress"

Fig.5

Relative expression of SiASR family genes in roots, stems and leaves after 10% PEG-6000 stress treatment"

Fig.6

Relative expression of SiASR family genes in roots, stems and leaves after salt stress"

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