Crops ›› 2022, Vol. 38 ›› Issue (3): 92-98.doi: 10.16035/j.issn.1001-7283.2022.03.013

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The Effects of Different Night Temperature Treatments on in vitro Tuberization and Related-Genes Expression in Potato

Liu Ju(), Li Guangcun, Duan Shaoguang, Hu Jun, Jian Yinqiao, Liu Jiangang, Jin Liping, Xu Jianfei()   

  1. Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences/Key Laboratory of Biology and Genetic Improvement of Tuber and Root Crops, Ministry of Agriculture and Rural Affairs, Beijing 100081, China
  • Received:2021-04-30 Revised:2021-05-27 Online:2022-06-15 Published:2022-06-20
  • Contact: Xu Jianfei E-mail:liuju413@163.com;xujianfei@caas.cn

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Abstract:

In order to investigate the effects of different night-time temperature treatments on in vitrotuberization in potato, the initial time, percentage and type of in vitrotuberization were recorded using seven potato varieties or materials induced cultured at day/night temperature of 23℃/13℃, 23℃/18℃ and 23℃/23℃. The expression changes in tuberization-related genes were also analysed using initially microtuber formed and non-formed plants. The results showed that for most potato genotypes, compared with other treatments, 23℃/13℃ treatment significantly prolonged the initial time of in vitrotuberization, 23℃/23℃ treatment reduced the percentage of in vitrotuberization, 23℃/18℃ treatment tuberization time was early and the average percentage was 84.3%, the most conducive to the formation of test in vitrotuberization. Potato in vitrotuberization formation was affected on night temperature through regulating gene expression of circadian clock, sugar metabolism and photoperiodic signaling pathway, such as StFKF1, StCO1and StSWEET1.

Key words: Potato, In vitro tuberization, Night temperature, Initiation time of tuberization, Gene expression

Fig.1

Schematic diagram of types of in vitro tuberization in ‘RH’ The red arrow points to in vitro tuberization, bar=1cm"

Table 1

RT-qPCR primer information"

基因名称
Gene name		 基因
Gene		 功能
Function		 引物序列（5’→3’）
Primer sequence (5’→3’)
StFKF1
PGSC0003DMT400051416
蓝光受体蛋白基因,光温信号感应
F：GCTCTACTGGTTATCGCGCT
R：GGGTCACTGTGCCATCATCA
StTOC1
PGSC0003DMT400083086
生物钟基因,昼夜节律调节
F：GGCATGTGCTTTCAACCAGG
R：CCTCAAACGTGGTCTCTGCT
StELF4
PGSC0003DMT400003078
生物钟基因,昼夜节律调节
F：CAGGTGCAGTCTGTGTTGGA
R：TTAGGCTCACGTTCTGCACC
StCO1
PGSC0003DMT400026065
CONSTANS家族成员,块茎形成负调节因子
F：GTAGCAACAATTGGGCAAGGG
R：AGTAAACGGTACATGTTGCGGA
StSP6A
PGSC0003DMG400023365
拟南芥FT同源基因,块茎形成正调节因子
F：ACAGTGTATGCCCCAGGTTG
R：AACAGCTGCAACAGGCAATC
StSEX4
PGSC0003DMT400039423
蛋白络氨酸磷酸酶基因,参与淀粉降解
F：GCCGATCACTTCTCCCAACA
R：TTCCGTATTGCTGCACTGGT
StSWEET1
PGSC0003DMG400024818
蔗糖转运蛋白基因,调节蔗糖流出
F：GTCTGCCATTTGTGTCACCA
R：AATGCCTCAAGTCCTGCTCC
EF1α
PGSC0003DMT400059830
荧光定量内参基因
F：ATTGGAAACGGATATGCTCCA
R：TCCTTACCTGAACGCCTGTCA

Fig.2

Effects on initiation of in vitro tuberization under different night temperature treatments Different lowercase letters indicate significant difference at P < 0.05 level within the same genotype under different day/night temperature treatments, the same below"

Fig.3

Effects on percentage of in vitro tuberization under different night temperature treatments"

Fig.4

Effects on type of in vitro tuberization under different night temperature treatments"

Fig.5

Expression analysis of tuberization related genes under different day/night temperature treatments PT: plants with in vitro tuberization, NPT: plants without in vitro tuberization"

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