Crops ›› 2023, Vol. 39 ›› Issue (2): 16-23.doi: 10.16035/j.issn.1001-7283.2023.02.003

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Progress in the Technical Principle, Commercialization and Testing Research of Gene Edited Crops

Dou Yinggang1(), Zhen Zhen2()   

  1. 1School of Chemistry and Chemical Engineering, Shihezi University/State Key Laboratory Incubation Base for Green Processing of Chemical Engineering, Shihezi 832003, Xinjiang,China
    2Qiqihar Integrated Laboratory of Harbin Customs Technology Center, Qiqihar 161000, Heilongjiang, China
  • Received:2022-03-25 Revised:2022-07-26 Online:2023-04-15 Published:2023-04-11

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Abstract:

Since the introduction of gene editing technology in 2012, it has attracted widespread attention and continues to break new ground in terms of application prospects. This technology uses “molecular scissors” to break DNA double strands and attach non-homologous ends or homologous recombination to mutate, knock out, insert or replace specific sites in DNA. This paper detailed the principles and technical methods of various gene editing technologies to date, explained the current application of the technology and the progress of commercialization of the edited crops, and analyzed the detection studies of the technology. Gene editing technology has become a global hotspot, new technologies and new research results are springing up and being applied in the fields of plant and animal breeding, disease treatment, drug development, etc. In the future, this technology will definitely show its revolutionary significance, and how to regulate, detect, and commercialize the application will become an urgent problem to be solved by the regulatory authorities.

Key words: Gene editing, Principle, Application, Regulation, Detection

Fig.1

Cre-lox mediated schematic"

Fig.2

Schematic diagram of ZFNs"

Fig.3

The principle of TALEN"

Fig.4

The principle of CRISPR/Cas9"

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