酿酒专用糯红高粱内生细菌多样性分析及功能菌株筛选

doi:10.16035/j.issn.1001-7283.2024.04.025

Abstract

Abstract:

The diversity and community structure of endophytic bacteria in waxy sorghum for brewing were examined and analyzed using pure culture in order to examine the functional traits and community structure of endophytic bacteria of various varieties and tissues from waxy sorghum for brewing during different phenological periods and the choice of culture medium was used to filter the functioning bacteria. The results showed that 183 bacteria were isolated from the hybrid Jinnuoliang 1 and the local variety Qingkeyang, belonging to two phyla, five classes and 17 genera and 39 species. The dominant phyla was Proteobacteria, accounting for 85.79%, and the dominant class was γ-Protea, accounting for 70.49%, the dominant genus was Pseudomonas, accounting for 43.72%, and the dominant species was Pseudomonas sp., accounting for 33.33%. Therefore, endophytic bacteria from waxy sorghum were diverse in varieties and rich in functions. These research indicated that there are significant differences in the composition of unique endophytic bacterial populations among different varieties and tissues from waxy sorghum for brewing during different phenological periods.

Key words: Brewing sorghum, Waxy sorghum, Endophytic bacteria, Community structure, Diversity

Hu Lianqing, Chen Lu, Liu Wenwen, Zhou Wanhai, Feng Ruizhang, Wei Qin, Zhao Xin, Shu Hao, Chen Lingmei, Chen Yuwei. Investigation on the Diversity of Endophytic Bacteria and Screening of Functional Strains in Waxy Sorghum for Brewing[J].Crops, 2024, 40(4): 194-202.

Figures/Tables 6

Table 1

Table 2

Composition of endophytic bacteria in waxy sorghum"

相似性最高菌株 The most similar strain	金糯梁1号Jinnuoliang 1																	青壳洋Qingkeyang
	苗期 Seedling stage							花期 Florescence stage						蜡熟期 Waxy stage				苗期 Seedling stage					花期 Florescence stage						蜡熟期 Waxy stage
	根 Root			茎 Stem		叶 Leaf		根 Root		茎 Stem		叶 Leaf	花 Flower	根 Root	茎 Stem	叶 Leaf		根 Root		茎 Stem		叶 Leaf	根 Root	茎 Stem	叶 Leaf		花 Flower		根 Root		茎 Stem		叶 Leaf
醋酸钙不动杆菌 Acinetobacter calcoaceticus	-			-		-		-		-		-	-	-	-	-		-		-		-	1	-	-		-		-		-		-
不动杆菌Acinetobacter sp.	-			1		-		1		-		-	-	-	-	-		-		-		-	-	-	-		-		-		-		-
活不动杆菌Acinetobacter vivianii	-			-		-		-		-		-	-	-	-	-		-		-		-	4	-	-		-		-		-		-
堀越氏芽胞杆菌Bacillus horneckiae	-			-		-		-		-		-	-	-	-	-		-		-		-	-	-	-		-		1		-		-
同温层芽孢杆菌Bacillus stratosphericus	-			-		-		-		-		-	-	-	-	-		-		-		-	-	1	-		-		-		-		-
Bacterium	-			-		-		-		-		-	-	-	-	-		-		-		-	-	-	-		-		-		1		1
Bakterija	-			-		-		1		1		-	-	-	-	-		-		-		-	1	-	-		-		-		-		-
唐菖蒲伯克霍尔德氏菌 Burkholderia gladioli	-			-		-		-		-		-	-	-	1	-		-		-		-	-	-	-		-		-		2		-
伯克霍尔德氏菌Burkholderia sp.	-			-		-		-		-		-	-	1	-	-		-		-		-	-	-	-		-		-		1		1
热带伯克霍尔德氏菌 Burkholderia tropica	-			-		-		-		-		-	-	-	1	-		-		-		-	-	-	-		-		-		1		-
柠檬酸杆菌Citrobacter sp.	-			-		-		-		-		-	-	2	-	-		-		-		-	-	-	-		-		-		-		1
贪铜杆菌Cupriavidus sp.	-			-		-		-		-		-	-	-	-	-		-		-		-	1	-	-		-		-		-		-
阿氏肠杆菌Enterobacter asburiae	-			-		-		-		-		-	-	1	-	-		-		-		-	-	-	-		-		-		-		-
阴沟肠杆菌Enterobacter cloacae	-			-		-		-		-		-	-	-	-	-		-		-		-	-	-	-		-		1		-		-
霍氏肠杆菌Enterobacter hormaechei		-	-		-		-		-		-		-	-	-	-	-		-		-		1	-		-		-		1		2		-
科贝肠杆菌Enterobacter kobei		-	-		-		-		-		-		-	-	1	-	-		-		-		-	-		-		-		-		1		-
罗根肠杆菌 Enterobacter roggenkampii		-	-		-		-		-		-		-	3	-	-	-		-		-		-	-		-		-		10		7		-
肠棒杆菌Enterobacter sp.		-	-		-		-		-		-		-	1	1	1	-		-		-		4	-		-		-		-		4		-
香坊肠杆菌 Enterobacter xiangfangensis		-	-		-		-		-		-		-	-	-	-	-		-		-		-	-		-		-		-		1		-
γ蛋白细菌 Gamma proteobacterium symbiont		-	-		-		-		-		-		-	-	-	-	-		-		-		-	1		-		-		-		-		-
内生固氮菌 Herbaspirillum frisingense		-	-		-		-		-		-		-	-	-	-	-		-		-		-	1		-		-		-		-		-
哈特草螺菌Herbaspirillum huttiense		-	-		-		-		-		-		-	-	-	-	-		-		-		3	-		-		1		-		-		-
乌头内生菌Herbaspirillum sp.		-	-		-		-		-		-		-	-	-	-	-		-		-		2	-		-		-		-		-		-
Hrizaobakterijum sp.		-	-		-		-		2		-		-	-	-	-	-		-		-		-	-		-		-		-		-		-
肺炎克雷伯菌Klebsiella pneumoniae		-	-		-		-		-		-		-	-	-	-	-		-		-		-	-		-		-		-		1		-
克雷伯氏菌Klebsiella sp.		-	-		-		-		-		-		-	-	-	-	-		-		-		-	1		-		-		-		-		-
Komamonas aquatica		-	-		-		1		-		-		-	-	-	-	-		-		-		-	-		-		-		-		-		-
Metilobakterijum sp.		-	-		-		-		-		-		2	-	-	-	-		-		-		-	-		-		-		-		-		-
Nekulturna bakterija		-	-		-		2		-		-		1	-	-	-	-		-		-		-	-		-		-		-		-		-
Nekulturna beta proteobakterijum		-	-		-		-		-		-		-	-	-	-	-		-		-		3	-		-		2		-		-		-
Nekulturni Citrobacter sp.		-	-		-		1		-		-		-	-	-	-	-		-		-		2	-		-		-		-		-		-
叶杆菌 Phyllobacterium brassicacearum		-	-		-		1		-		-		-	-	-	-	-		-		-		-	-		-		-		-		-		-
绿针假单胞菌 Pseudomonas chlororaphis		-	-		-		-		1		-		-	-	-	-	-		-		-		-	-		-		-		-		-		-
荧光假单胞菌 Pseudomonas fluorescens		-	-		-		-		-		-		-	1	-	-	-		-		-		-	-		-		-		-		-		-
湖南假单胞菌 Pseudomonas hunanensis		-	-		-		-		-		-		-	1	-	-	-		-		-		-	-		-		-		-		-		-
浅黄色假单胞菌Pseudomonas lurida		-	-		-		-		3		-		-	-	-	-	-		-		-		-	-		-		-		-		-		-
蒙氏假单胞菌Pseudomonas monteilii		-	-		-		-		1		-		-	2	-	-	-		-		-		-	-		-		-		-		-		-
变形假单胞菌 Pseudomonas plecoglossicida		-	-		-		-		-		-		-	2	-	-	-		-		-		-	-		-		-		-		-		-
恶臭假单胞菌Pseudomonas putida		-	-		1		-		4		-		-	-	-	-	-		1		-		-	-		-		-		1		1		-
假单胞菌Pseudomonas sp.		-	12		3		6		10		1		-	3	-	-	3		2		-		13	3		-		2		1		-		-
葡萄牙根瘤菌Rhizobium lusitanum		2	-		-		-		-		-		-	-	-	-	-		-		-		1	-		-		-		-		-		-
根瘤菌Rhizobium sp.		-	-		-		-		-		-		-	-	-	-	-		-		-		-	-		-		-		1		-		-
鞘氨醇单胞菌Sphingomonas echinoides		-	-		2		-		-		-		-	-	-	-	-		-		-		-	-		-		-		-		-		-
Uncultured bacterium		-	1		-		-		-		-		-	-	-	-	-		2		-		-	-		-		-		1		2		-

Table 2

Table 3

Fig.1

Fig.2

Table 4

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植物组织Plant tissue	品种Variety	生育期Growth stage	定殖率Colonization rate	分离率Separation rate
根Root	金糯粱1号	苗期、花期、蜡熟期	25.33	21.33
	青壳洋	苗期、花期、蜡熟期	33.33	37.33
茎Stem	金糯粱1号	苗期、花期、蜡熟期	8.00	26.67
	青壳洋	苗期、花期、蜡熟期	18.67	24.00
叶Leaf	金糯粱1号	苗期、花期、蜡熟期	4.00	5.33
	青壳洋	苗期、花期、蜡熟期	3.33	2.00
花Flower	金糯粱1号	花期	6.00	6.00
	青壳洋	花期	6.00	10.00

项目 Item	品种Variety		组织Tissue				生育期Growth stage
项目 Item	金糯粱1号 Jinnuoliang 1	青壳洋 Qingkeyang	根 Root	茎 Stem	叶 Leaf	花 Flower	苗期 Seedling stage	花期 Florescence stage	蜡熟期 Waxy stage
丰富度指数Abundance degree (S)	23	27	36	21	6	6	5	22	18
香农指数Shannon-Wiener index (H)	2.2249	2.6459	2.5629	2.3943	1.5811	1.7481	0.9321	2.4410	2.5648
优势度指数Dominance degree (D)	0.7529	0.8822	0.8590	0.8386	0.7407	0.8200	0.4400	0.8160	0.8728

Investigation on the Diversity of Endophytic Bacteria and Screening of Functional Strains in Waxy Sorghum for Brewing

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菌株 Bacterial strain	产淀粉酶 Amylase production	产蛋白酶 Protease production	产脂肪酶 Lipase production	产果胶酶 Pectinase production	产纤维素酶 Cellulase production	产酯酶 Esterase production	固氮能力 Nitrogen fixation	溶磷能力 Phosphate solubilization
不动杆菌Acinetobacter sp.	-	-	+	-	-	-	-	-
活不动杆菌Acinetobacter vivianii	-	-	+	-	-	-	-	-
唐菖蒲伯克霍尔德氏菌Burkholderia gladioli	+	-	+	-	+	-	-	-
伯克霍尔德氏菌Burkholderia sp.	-	-	-	-	-	+	-	-
热带伯克霍尔德氏菌Burkholderia tropica	-	-	+	-	-	+	-	-
柠檬酸杆菌Citrobacter sp.	-	-	-	-	-	-	-	+
贪铜杆菌Cupriavidus sp.	+	-	+	-	-	-	-	-
阿氏肠杆菌Enterobacter asburiae	-	-	-	-	-	-	+	-
科贝肠杆菌Enterobacter kobei	+	-	-	-	-	-	-	-
罗根肠杆菌Enterobacter roggenkampii	-	+	-	+	-	+	+	-
肠棒杆菌Enterobacter sp.	+	-	-	-	-	-	-	-
香坊肠杆菌Enterobacter xiangfangensis	-	-	+	-	-	+	-	-
肺炎克雷伯菌Klebsiella pneumoniae	-	-	-	-	-	-	-	+
Komamonas aquatica	+	-	-	-	-	-	-	-
Metilobakterijum sp.	+	-	+	-	-	-	-	-
浅黄色假单胞菌Pseudomonas lurida	+	-	+	-	-	+	-	-
蒙氏假单胞菌Pseudomonas mosselii	-	+	-	+	-	-	-	-
恶臭假单胞菌Pseudomonas putida	-	+	+	+	-	-	-	-
假单胞菌Pseudomonas sp.	+	+	-	+	-	+	+	-
根瘤菌Rhizobium sp.	-	-	-	-	-	-	+	-

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