作物杂志,2019, 第1期: 22–31 doi: 10.16035/j.issn.1001-7283.2019.01.004

所属专题: 其他作物

• 遗传育种·种质资源·生物技术 • 上一篇    下一篇

基于SSR标记的80份烟草种质指纹图谱的构建及遗传多样性分析

陈芳,徐世晓,李晓辉,刘超,周建飞,王袁,田培,杨铁钊   

  1. 河南农业大学烟草学院,450002,河南郑州
  • 收稿日期:2018-10-09 修回日期:2018-11-23 出版日期:2019-02-15 发布日期:2019-02-01
  • 通讯作者: 杨铁钊
  • 基金资助:
    河南省自然科学基金(162300410144);中国烟草总公司河南省公司重大专项(2018410000270035)

Construction of Molecular Fingerprinting and Analysis of Genetic Diversity for 80 Tobacco (Nicotiana tabacum) Germplasms Based on SSR Markers

Fang Chen,Shixiao Xu,Xiaohui Li,Chao Liu,Jianfei Zhou,Yuan Wang,Pei Tian,Tiezhao Yang   

  1. College of Tobacco Science, Henan Agricultural University, Zhengzhou 450002, Henan, China
  • Received:2018-10-09 Revised:2018-11-23 Online:2019-02-15 Published:2019-02-01
  • Contact: Tiezhao Yang

摘要:

利用均匀分布于烟草24个连锁群上的48个SSR标记对80份烟草材料进行分析。结果显示,48个SSR标记共扩增出211个等位基因,平均每个标记4.396个,Shannon′s信息指数I为1.034,多态信息含量值为0.229~0.905,观测杂合度(Ho)、期望杂合度(He)和Nei′s多样性指数(H)平均值分别为0.320、0.572、0.431。聚类结果表明,在遗传距离为0.68时可以将80份烟草种质分为2个类群。5个烟草居群间的遗传一致度在0.643~0.765范围内,遗传距离分布在0.268~0.442。筛选4对核心引物构建了不同烟草种质资源的数字指纹图谱, 可将这80份烟草种质资源全部区分开。本研究在分子水平上为筛选优质烟草种质资源、挖掘重要基因以及拓宽烟草育种遗传基础等工作提供科学依据。

关键词: 烟草, SSR标记, 指纹图谱, 遗传多样性, 种质资源

Abstract:

80 tobacco germplasm resources were studied using 48 simple sequence repeat (SSR) markers distributed uniformly over 24 linkage groups of tobacco. Results showed that a total of 211 alleles among these germplasm were identified at all of 48 SSR markers loci, and an average of 4.396 alleles was observed for each primer pair with average heterozygosity (Ho) 0.320, average expected heterozygosity (He) 0.572. Shannon′s index was 1.034, Nei′s index was 0.431, and the PIC ranged from 0.229 to 0.905. These germplasm were divided into two groups at a genetic similarity coefficient of 0.68 by UPGMA cluster analysis. The genetic identity among the five tobacco populations ranged from 0.643 to 0.765, and the genetic distance ranged from 0.268 to 0.442. Four optimal pairs of core primers were used for the DNA fingerprint construction. The study can provide reference for further tobacco germplasm resources search which is important for genes discovery and exploitation.

Key words: Tobacco, Simple sequence repeat (SSR), Fingerprint, Genetic diversity, Germplasm resources

表1

供试烟草材料"

编号Code 材料名称Material name 类型Type 来源Origin 系谱Pedigree
1 大黄金多叶Dahuangjinduoye 烤烟Flue-cured tobacco 中国山东 Mammoth Golden
2 革新一号Gexin No.1 烤烟Flue-cured tobacco 中国山东 Mammoth Golden
3 革新五号Gexin No.5 烤烟Flue-cured tobacco 中国山东 Mammoth Golden
4 寸茎烟Cunjingyan 烤烟Flue-cured tobacco 中国云南 Mammoth Golden
5 红花大金元Honghuadajinyuan 烤烟Flue-cured tobacco 中国云南 Mammoth Golden
6 闵烟四号Minyan No.4 烤烟Flue-cured tobacco 中国福建 特字401×塘蓬
7 闵烟二号Minyan No.2 烤烟Flue-cured tobacco 中国福建 特字400×特字401
8 广黄五十五号Guanghuang No.55 烤烟Flue-cured tobacco 中国广东 广黄10号×(金星6007×抵字101)
9 广黄10号Guanghuang No.10 烤烟Flue-cured tobacco 中国广东 特字401×北流一号
10 K394 烤烟Flue-cured tobacco 美国 Speight G-28×McNair944
11 豫烟8号Yuyan No.8 烤烟Flue-cured tobacco 中国河南 NC89
12 中烟86 Zhongyan86 烤烟Flue-cured tobacco 中国山东 Speight G-28×净叶黄
13 革新三号Gexin No.3 烤烟Flue-cured tobacco 中国山东 7411×长脖黄
14 NC82 烤烟Flue-cured tobacco 美国 6129×Coke319
15 NC27NF 烤烟Flue-cured tobacco 美国 (Coker319×NCTG21)×Coker319
16 NC37NF 烤烟Flue-cured tobacco 美国 (Coker319×NCTG82)×NC82
17 Coker176 烤烟Flue-cured tobacco 美国 Coker258×Coker319×Coker139
18 Oxford 4 烤烟Flue-cured tobacco 美国 (Florida 301×Warne)×Warne
19 山东多叶Shandongduoye 烤烟Flue-cured tobacco 中国山东 特字402
20 RG89 烤烟Flue-cured tobacco 美国 K326×Coker319×Coker411
21 豫烟7号Yuyan No.7 烤烟Flue-cured tobacco 中国河南 MSSpeight G-28×664
22 豫烟11号Yuyan No.11 烤烟Flue-cured tobacco 中国河南 MSK326×8306
23 豫烟5号Yuyan No.5 烤烟Flue-cured tobacco 中国河南 (Speight G-28×红花大金元)×(净叶黄×NC89)
24 云烟2号Yunyan No.2 烤烟Flue-cured tobacco 中国云南 红花大金元×Speight G-28
25 中烟14 Zhongyan14 烤烟Flue-cured tobacco 中国山东 金星6007×Speight G-28
26 中烟103 Zhongyan103 烤烟Flue-cured tobacco 中国山东 红花大金元
27 Dixie Bright101 烤烟Flue-cured tobacco 美国 [(Ti448A×400)F3×(400×Florida 301)]BC2F3
28 Vesta 64 烤烟Flue-cured tobacco 美国 Oxford4×Yellow Special
29 南江3号Nanjiang No.3 烤烟Flue-cured tobacco 中国贵州 红花大金元
30 Speight G-140 烤烟Flue-cured tobacco 美国 Speight G-7×Speight G-3
31 Coker 258 烤烟Flue-cured tobacco 美国 Coker319×NC95
32 Oxford 1 烤烟Flue-cured tobacco 美国 (Florida 301×Virginia Leaf)×Virginia Leaf
33 豫烟9号Yuyan No.9 烤烟Flue-cured tobacco 中国河南 MS中烟98×664-1
34 中烟15 Zhongyan15 烤烟Flue-cured tobacco 中国山东 单育二号×Speight G-28
35 RG8 烤烟Flue-cured tobacco 美国 K326×K399
36 Coker 319 烤烟Flue-cured tobacco 美国 Coker139×Hicks
37 G-28 烤烟Flue-cured tobacco 美国 (Oxford1-181×Coker139)×NC 95
38 Coker 298 烤烟Flue-cured tobacco 美国 Coker139×Coker156
39 NC95 烤烟Flue-cured tobacco 美国 Coker139×Bell 4-30
40 NC89 烤烟Flue-cured tobacco 美国 (NC95×Hicks)
41 K326 烤烟Flue-cured tobacco 美国 McNair225(McNair30×NC95)
42 净叶黄Jingyehuang 烤烟Flue-cured tobacco 中国河南 长脖黄
43 庆胜2号Qingsheng No.2 烤烟Flue-cured tobacco 中国河南 净叶黄
44 长脖黄Changbohuang 烤烟Flue-cured tobacco 中国河南 不详
45
K149
烤烟Flue-cured tobacco
美国
(Speight G-28×Coker254)×(CB139×F-105)×
(Speight G-28×Coker254)×McN399
46 豫烟6号Yuyan No.6 烤烟Flue-cured tobacco 中国河南 MSK326×农大202
47 NC2326 烤烟Flue-cured tobacco 美国 9102×Hicks
48 中烟90 Zhongyan90 烤烟Flue-cured tobacco 中国山东 (单育2号×Speight G-28)×(Speight G-28×净叶黄)
49 豫烟12号Yuyan No.12 烤烟Flue-cured tobacco 中国河南 MSSpeight G-28×YZ90
编号Code 材料名称Material name 类型Type 来源Origin 系谱Pedigree
50 Mammoth Golden 烤烟Flue-cured tobacco 美国 Yellow Mammoth×Gold Dollar
51 秦烟96 Qinyan96 烤烟Flue-cured tobacco 中国陕西 G-28×净叶黄
52 秦烟98 Qinyan98 烤烟Flue-cured tobacco 中国陕西 秦烟95×CV70
53 韭菜坪2号Jiucaiping No.2 烤烟Flue-cured tobacco 中国贵州 G28
54 湘烟3号Xiangyan No.3 烤烟Flue-cured tobacco 中国湖南 MSYZ206-9×YZ94-7
55 翠碧1号Cuibi No.1 烤烟Flue-cured tobacco 中国福建 特字401
56 密叶尖Miyejian 烤烟Flue-cured tobacco 中国福建 Mammoth Golden
57 吉烟7号Jiyan No.7 烤烟Flue-cured tobacco 中国吉林 CVl6-1×吉烟一号
58 豫烟10号Yuyan No.10 烤烟Flue-cured tobacco 中国河南 农大201×云烟87
59 Coker187-Hicks 烤烟Flue-cured tobacco 美国 Coker187×Coker156
60 洛宁1号Luoning No.1 烤烟Flue-cured tobacco 中国河南 不详
61 Burley21 白肋烟White burley 美国 不详
62 Burley37 白肋烟White burley 美国 不详
63 KY10 白肋烟White burley 美国 不详
64 KY14 白肋烟White burley 美国 不详
65 金水白肋一号Jinshuibaile No.1 白肋烟White burley 中国湖北 Burley21
66 护脖香Huboxiang 晒烟Sun-cured tobacco 中国黑龙江 不详
67 小护脖香Xiaohuboxiang 晒烟Sun-cured tobacco 中国黑龙江 不详
68 大红花Dahonghua 晒烟Sun-cured tobacco 中国黑龙江 不详
69 金英Jinying 晒烟Sun-cured tobacco 中国广东 不详
70 青梗Qinggeng 晒烟Sun-cured tobacco 中国广东 不详
71 Alidat0bak 雪茄烟Cigar 未知 不详
72 Pennbel69 雪茄烟Cigar 未知 不详
73 Florida301 雪茄烟Cigar 美国 不详
74 Ambale-Ma 雪茄烟Cigar 美国 不详
75 Beinhart 1000-1 雪茄烟Cigar 多米尼加 不详
76 巴斯马Basma 香料烟Turkish tobacco 阿尔巴尼亚 不详
77 Vranjska Jaka 香料烟Turkish tobacco 南斯拉夫 不详
78 A37 香料烟Turkish tobacco 未知 不详
79 沙姆逊Bashi Bagli 香料烟Turkish tobacco 土耳其 不详
80 Adcook 香料烟Turkish tobacco 印度 不详

表2

48对SSR引物在80份烟草材料中的遗传多样性"

引物名称
Primer
染色体
Chromosome
位置
(Mb)
Position
多态性
信息含量
PIC
基因型数
Genotypes
等位
基因数
Na
有效等位
基因数
Ne
Shannon′s
信息指数
I
观测
杂合度
Ho
期望
杂合度
He
平均Nei′s
多样性指数
H
PT30477 1 18.922 0.288 3 2 1.536 0.533 0.050 0.351 0.334
PT30245 1 68.842 0.905 18 15 11.278 2.566 0.175 0.917 0.615
PT52378 2 18.535 0.374 3 2 1.989 0.690 0.150 0.500 0.423
PT61027 2 68.974 0.446 6 3 2.041 0.863 0.525 0.513 0.530
PT54293 3 19.641 0.544 9 4 2.410 1.107 0.525 0.589 0.501
PT50680 3 70.072 0.864 16 10 8.112 2.176 0.200 0.882 0.639
PT50267 4 18.401 0.229 3 2 1.358 0.433 0.088 0.265 0.237
PT1140 4 69.693 0.370 3 2 1.963 0.684 0.838 0.494 0.492
PT55416 5 20.022 0.326 3 2 1.694 0.600 0.175 0.412 0.256
PT60352 5 68.405 0.365 3 2 1.923 0.673 0.325 0.483 0.292
PT30229 6 19.479 0.362 3 2 1.904 0.668 0.150 0.478 0.060
PT60236 6 68.341 0.792 24 20 5.100 2.261 0.175 0.809 0.600
PT60619 7 18.954 0.357 3 2 1.871 0.658 0.163 0.469 0.303
PT61257 7 72.150 0.368 3 2 1.949 0.680 0.263 0.490 0.426
PT52991 8 18.884 0.846 19 15 6.957 2.329 0.463 0.862 0.509
PT61104 8 67.718 0.361 3 2 1.893 0.665 0.013 0.475 0.126
PT54729 9 17.556 0.375 3 2 1.999 0.693 0.250 0.503 0.392
PT60681 9 68.839 0.368 3 2 1.949 0.680 0.113 0.490 0.272
PT53083 10 19.743 0.536 5 3 2.599 1.013 0.138 0.619 0.572
PT60191 10 68.441 0.794 13 11 5.276 2.000 0.175 0.816 0.454
PT60305 11 19.846 0.353 3 2 1.847 0.651 0.288 0.462 0.367
PT30420 11 68.146 0.355 3 2 1.859 0.655 0.450 0.465 0.451
PT60771 12 16.428 0.589 5 3 2.968 1.093 0.213 0.667 0.482
PT61315 12 67.946 0.892 22 19 9.900 2.608 0.475 0.905 0.709
PT50610 13 14.433 0.319 3 2 1.663 0.588 0.050 0.401 0.211
PT61426 13 67.847 0.375 3 2 2.000 0.693 0.125 0.503 0.416
PT51071 14 17.850 0.437 6 3 1.970 0.847 0.175 0.495 0.407
PT55360 14 69.907 0.377 5 3 1.706 0.747 0.063 0.417 0.400
PT53913 15 18.143 0.581 6 3 2.897 1.081 0.338 0.659 0.410
引物名称
Primer
染色体
Chromosome
位置
(Mb)
Position
多态性
信息含量
PIC
基因型数
Genotypes
等位
基因数
Na
有效等位
基因数
Ne
Shannon′s
信息指数
I
观测
杂合度
Ho
期望
杂合度
He
平均Nei′s
多样性指数
H
PT60205 15 68.301 0.598 11 5 2.769 1.260 0.300 0.643 0.608
PT54123 16 18.149 0.487 5 3 2.245 0.931 0.113 0.558 0.410
PT30302 16 69.375 0.621 8 4 3.108 1.239 0.763 0.683 0.564
PT51291 17 18.242 0.540 6 3 2.625 1.020 0.338 0.623 0.326
PT53287 17 69.828 0.455 5 3 2.241 0.881 0.563 0.557 0.501
PT52654 18 18.897 0.530 4 3 2.534 1.003 0.938 0.609 0.529
PT50443 18 68.683 0.611 7 4 3.028 1.210 0.213 0.674 0.559
PT55357 19 20.934 0.538 5 4 2.448 1.091 0.688 0.595 0.501
PT54654 19 68.320 0.391 5 3 1.780 0.769 0.138 0.441 0.412
PT52961 20 16.958 0.549 6 3 2.640 1.032 0.075 0.625 0.421
PT51701 20 70.417 0.547 6 3 2.673 1.030 0.250 0.630 0.529
PT55114 21 18.065 0.488 7 4 2.126 0.998 0.238 0.533 0.471
PT20388 21 72.136 0.506 4 3 2.460 0.967 0.375 0.597 0.472
PT60967 22 18.999 0.375 3 2 1.997 0.692 0.913 0.502 0.492
PT52736 22 68.263 0.585 6 3 2.928 1.087 0.438 0.663 0.371
PT53474 23 18.788 0.358 5 4 1.807 0.692 0.525 0.449 0.276
PT53595 23 67.542 0.370 6 7 1.795 0.767 0.050 0.446 0.425
PT60715 24 22.068 0.374 2 2 1.995 0.692 0.950 0.502 0.500
PT30375 24 68.195 0.663 7 4 3.490 1.315 0.350 0.718 0.432
平均值 Mean 0.494 6.458 4.396 2.902 1.034 0.320 0.572 0.431

图1

PT53474引物部分扩增图谱 图中1~44泳道编号与表1材料编号相对应"

表3

用4对引物建立的80份烟草种质数字指纹图谱"

品种Variety 类型Type 数字指纹图谱Numbered fingerprint
大黄金多叶Dahuangjinduoye 烤烟Flue-cured tobacco 10011111 10010011 10010010 10010010 11101000 11101000 11001111 11001111
革新一号Gexin No.1 烤烟Flue-cured tobacco 10011111 10010011 10011011 10011011 11101000 11101000 11001111 11001111
革新五号Gexin No.5 烤烟Flue-cured tobacco 10011111 10010011 10100001 10100001 11101000 11101000 11001111 11001111
寸茎烟Cunjingyan 烤烟Flue-cured tobacco 10001011 10001011 10100001 10100001 11101000 11101000 11001111 11001111
红花大金元Honghuadajinyuan 烤烟Flue-cured tobacco 10001011 10001011 10100001 10100001 11101000 11101000 11001111 11001111
闵烟四号Minyan No.4 烤烟Flue-cured tobacco 10010001 10010001 10011100 10011100 11101100 11101000 11001111 11001111
闵烟二号Minyan No.2 烤烟Flue-cured tobacco 10010001 10010001 10011100 10011100 11101100 11101000 11001111 11001111
广黄五十五号Guanghuang No.55 烤烟Flue-cured tobacco 10010001 10010001 10011100 10011100 11101100 11101000 11001111 11001111
广黄10号Guanghuang No.10 烤烟Flue-cured tobacco 10010001 10010001 10010010 10011100 11101100 11101000 11010010 11010010
K394 烤烟Flue-cured tobacco 10010001 10010001 10011100 10011100 11101100 11101000 11010010 11010010
豫烟8号Yuyan No.8 烤烟Flue-cured tobacco 10011011 10011011 10011100 10011100 11101000 11101000 11010000 11010000
中烟86 Zhongyan86 烤烟Flue-cured tobacco 10011011 10011011 10010110 10010110 11100101 11100101 11010000 11010000
革新三号Gexin No.3 烤烟Flue-cured tobacco 10011011 10011011 10011100 10011100 11100110 11100110 11010000 11010000
NC82 烤烟Flue-cured tobacco 10010010 10010010 10011010 10011010 11110001 11110001 11010011 11010011
NC27NF 烤烟Flue-cured tobacco 10010010 10010010 10010110 10010110 11100111 11100111 11010011 11010011
NC37NF 烤烟Flue-cured tobacco 10010010 10010010 10010110 10010110 11110001 11110001 11010011 11010011
Coker176 烤烟Flue-cured tobacco 10010010 10010010 10011010 10011010 11110001 11110001 11010011 11010011
Oxford 4 烤烟Flue-cured tobacco 10011110 10011110 10011010 10011010 11101000 11101000 11100010 11100010
山东多叶Shandongduoye 烤烟Flue-cured tobacco 10011110 10011110 10011010 10011010 11101000 11101000 11100010 11100010
RG89 烤烟Flue-cured tobacco 10010100 10010100 10010010 10010010 11101000 11100110 11010111 11010111
豫烟7号Yuyan No.7 烤烟Flue-cured tobacco 10010100 10010100 10010111 10011100 11101000 11101000 11010111 11010111
豫烟11号Yuyan No.11 烤烟Flue-cured tobacco 10010100 10010100 10011100 10011100 11101000 11101000 11010111 11010111
豫烟5号Yuyan No.5 烤烟Flue-cured tobacco 10010011 10010011 10010010 10100001 11101000 11101000 11010000 11010000
云烟2号Yunyan No.2 烤烟Flue-cured tobacco 10010011 10010011 10011100 10011100 11110010 11110010 11010000 11001110
中烟14 Zhongyan14 烤烟Flue-cured tobacco 10010011 10010011 10011100 10011100 11111100 11111100 11010000 11001110
中烟103 Zhongyan103 烤烟Flue-cured tobacco 10010011 10010011 10100001 10100001 11101001 11101000 11010000 11010101
Dixie Bright101 烤烟Flue-cured tobacco 10010011 10010011 10100001 10100001 11101010 11100000 11010000 11010101
Vesta 64 烤烟Flue-cured tobacco 10011111 10011000 10100001 10100001 11100000 11100000 11010000 11010101
南江3号Nanjiang No.3 烤烟Flue-cured tobacco 10011111 10011111 10011011 10011011 11101011 11101011 11010000 11010101
Speight G-140 烤烟Flue-cured tobacco 10011111 10011111 10011011 10011011 11101011 11101011 11010000 11001110
Coker 258 烤烟Flue-cured tobacco 10010010 10010010 10011001 10011001 11111100 11111100 11001110 11010110
Oxford 1 烤烟Flue-cured tobacco 10011111 10011000 10010010 10010010 11111100 11111100 11001110 11010110
豫烟9号Yuyan No.9 烤烟Flue-cured tobacco 10010111 10010111 10011000 10011000 11101000 11101000 11011000 11011000
中烟15 Zhongyan15 烤烟Flue-cured tobacco 10010111 10010111 10011000 10011000 11101000 11101000 11010000 11001110
RG8 烤烟Flue-cured tobacco 10010111 10010111 10011000 10011000 11101000 11101000 11011001 11011001
Coker 319 烤烟Flue-cured tobacco 10010111 10010111 10010010 10100001 11101000 11101000 11011001 11011001
G-28 烤烟Flue-cured tobacco 10010111 10010111 10011000 10011000 11101000 11101000 11001110 11010110
Coker 298 烤烟Flue-cured tobacco 10011111 10011111 10011001 10011001 11110010 11101100 11010000 11001110
NC95 烤烟Flue-cured tobacco 10011100 10011100 10011001 10011001 11110010 11110010 11011000 11011000
NC89 烤烟Flue-cured tobacco 10011011 10011011 10011001 10011001 11101101 11101101 11010001 11010001
K326 烤烟Flue-cured tobacco 10011011 10011011 10011001 10011001 11101101 11101101 11010001 11010001
净叶黄Jingyehuang 烤烟Flue-cured tobacco 10010010 10010010 10011011 10011011 11101000 11101000 11010000 11010000
庆胜2号Qingsheng No.2 烤烟Flue-cured tobacco 10011100 10011100 10100001 10011100 11101000 11101000 11011000 11011000
长脖黄Changbohuang 烤烟Flue-cured tobacco 10100000 10100000 10011010 10011010 11101110 11101110 11001110 11011001
K149 烤烟Flue-cured tobacco 10100000 10100000 10011010 10011010 11101110 11101110 11100001 11010000
豫烟6号Yuyan No.6 烤烟Flue-cured tobacco 10100000 10100000 10100000 10100000 11101000 11101000 11100001 11010000
NC2326 烤烟Flue-cured tobacco 10011111 10011111 10100001 10011100 11101111 11101111 11010000 11001110
中烟90 Zhongyan90 烤烟Flue-cured tobacco 10100001 10100001 10011000 10011000 11101000 11101000 11010000 11010000
豫烟12号Yuyan No.12 烤烟Flue-cured tobacco 10100001 10100001 10011000 10011000 11101000 11101000 11001110 11011001
Mammoth Golden 烤烟Flue-cured tobacco 10011110 10011110 10010110 10010110 11101000 11101000 11010010 11010000
品种Variety 类型Type 数字指纹图谱Numbered fingerprint
秦烟96 Qinyan96 烤烟Flue-cured tobacco 10100001 10100001 10010110 10010110 11100000 11100000 11010000 11010100
秦烟98 Qinyan98 烤烟Flue-cured tobacco 10011111 10011111 10011000 10011000 11101111 11101111 11011111 11011111
韭菜坪2号Jiucaiping No.2 烤烟Flue-cured tobacco 10011000 10011000 10010110 10010110 11100000 11100000 11011001 11001110
湘烟3号Xiangyan No.3 烤烟Flue-cured tobacco 10100001 10100001 10010110 10010110 11101000 11101000 11011111 11011111
翠碧1号Cuibi No.1 烤烟Flue-cured tobacco 10011000 10011000 10011011 10011011 11100000 11100000 11010000 11010100
密叶尖Miyejian 烤烟Flue-cured tobacco 10011111 10011000 10011000 10011000 11100000 11100000 11010111 11010111
吉烟7号Jiyan No.7 烤烟Flue-cured tobacco 10011000 10011000 10011000 10011000 11101011 11101011 11010010 11011100
豫烟10号Yuyan No.10 烤烟Flue-cured tobacco 10011111 10011111 10100000 10100000 11110011 11110011 11100001 11010000
Coker187-Hicks 烤烟Flue-cured tobacco 10010101 10010101 10100001 10011011 11110011 11110011 11011001 11001110
洛宁1号Luoning No.1 烤烟Flue-cured tobacco 10011111 10011000 10010010 10010010 11110011 11110011 11011001 11001110
Burley21 白肋烟White burley 10011000 10011000 10010110 10010110 11110010 11110010 11100001 11010000
Burley37 白肋烟White burley 10011000 10011000 10010010 10010010 11100000 11100000 11011101 11010000
KY10 白肋烟White burley 10011111 10011000 10010010 10010010 11110111 11110111 11011101 11010000
KY14 白肋烟White burley 10011000 10011000 10100001 10011011 11100000 11100000 11010000 11010100
金水白肋一号Jinshuibaile No.1 白肋烟White burley 10011000 10011000 10010010 10010010 11101000 11100000 11011000 11011000
护脖香Huboxiang 晒烟Sun-cured tobacco 10010100 10010100 10100001 10100001 11111000 11101000 11011101 11010000
小护脖香Xiaohuboxiang 晒烟Sun-cured tobacco 10011111 10011000 10010010 10010010 11111000 11101000 11010010 11011100
大红花Dahonghua 晒烟Sun-cured tobacco 10011111 10011000 10100001 10100001 11101000 11100000 11100001 11010000
金英Jinying 晒烟Sun-cured tobacco 10011000 10011000 10010111 10011010 11101000 11101000 11100010 11100010
青梗Qinggeng 晒烟Sun-cured tobacco 10011111 10011111 10010010 10010010 11101000 11101000 11011100 11011100
Alidat0bak 雪茄烟Cigar 10010110 10010100 10010111 10011000 11100000 11100000 11010010 11010000
Pennbel69 雪茄烟Cigar 10001011 10001011 10010111 10011000 11111011 11111011 11100100 11100100
Florida301 雪茄烟Cigar 10011111 10010011 10010111 10011000 11111011 11111011 11010010 11010000
Ambale-Ma 雪茄烟Cigar 10001011 10001011 10010111 10011000 11100110 11100110 11100100 11100100
Beinhart 1000-1 雪茄烟Cigar 10011000 10011000 10010111 10011000 11111001 11111001 11011100 11011100
巴斯马Basma 香料烟Turkish tobacco 10010110 10010001 10010010 10010010 11100110 11100110 11010010 11010010
Vranjska Jaka 香料烟Turkish tobacco 10010101 10010101 10100001 10011100 11100000 11100000 11011011 11011011
A37 香料烟Turkish tobacco 10010110 10011100 10011010 10011010 11100000 11100000 11011011 11011011
沙姆逊Bashi Bagli 香料烟Turkish tobacco 10010101 10010101 10010010 10010010 11101010 11101000 11001111 11001111
Adcook 香料烟Turkish tobacco 10010101 10010101 10010010 10010010 11101010 11101000 11011011 11011011

图2

基于80份种质资源48个SSR标记的聚类分析 图中材料代号与表1材料编号相对应"

表4

烟草5个居群的遗传一致度(右上角)和遗传距离(左下角)"

类型Type 烤烟Flue-cured tobacco 白肋烟White burley 晒烟Sun-cured tobacco 雪茄烟Cigar 香料烟Turkish tobacco
烤烟Flue-cured tobacco 0.664 0.672 0.705 0.643
白肋烟White burley 0.409 0.713 0.721 0.677
晒烟 Sun-cured tobacco 0.398 0.339 0.711 0.765
雪茄烟Cigar 0.349 0.327 0.341 0.711
香料烟Turkish tobacco 0.442 0.390 0.268 0.341

图3

5个不同烟草居群的UPGMA聚类分析"

[1] 常爱霞, 贾兴华, 冯全福 , 等. 我国主要烤烟品种的亲源系谱分析及育种工作建议. 中国烟草科学, 2017(1):1-6.
doi: 10.3969/j.issn.1007-5119.2013.01.001
[2] 王元英, 周健 . 中美主要烟草品种亲源分析与烟草育种. 中国烟草学报, 1995,2(1):11-22.
[3] Bindler G, Van d H R,Gunduz I ,et al. A microsatellite marker based linkage map of tobacco. Theoretical & Applied Genetics, 2007,114(2):341-349.
doi: 10.1007/s00122-006-0437-5 pmid: 17115128
[4] 刘纪麟 . 玉米育种学. 北京: 中国农业出版社, 2000: 143-175.
[5] 郑殿升, 杨庆文, 刘旭 . 中国作物种质资源多样性. 植物遗传资源学报, 2011,12(4):497-500,506.
[6] Ren N, Timko M P . AFLP analysis of genetic polymorphism and evolutionary relationships among cultivated and wild Nicotiana species. Genome, 2001,44(4):559-571.
doi: 10.1139/g01-060 pmid: 11550889
[7] Julio E, Verrier J L, Borne F D D . Development of SCAR markers linked to three disease resistances based on AFLP within Nicotiana tabacum L. Theoretical & Applied Genetics, 2006,112(2):335-346.
doi: 10.1007/s00122-005-0132-y pmid: 16283232
[8] Deng S L, Huang Y L, He H H , et al. Genetic diversity of Aegiceras corniculatum (Myrsinaceae) revealed by amplified fragment length polymorphism (AFLP). Aquatic Botany, 2009,90(4):275-281.
doi: 10.1016/j.aquabot.2008.11.002
[9] Piano, L D, Abet M,Sorrentino C ,et al. Genetic variability in Nicotiana tabacum and Nicotiana species as revealed by RAPD markers:1. Development of the RAPD procedure. Beiträge Zur Tabakforschung, 2014,19(1):1-15.
doi: 10.2478/cttr-2013-0690
[10] Bai D, Reeleder R, Brandie J E . Identification of two RAPD markers tightly linked with the Nicotiana debneyi gene for resistance to black root rot of tobacco. Theoretical & Applied Genetics, 1995,91(8):1184-1189.
doi: 10.1007/BF00220927 pmid: 24170044
[11] 祁建民, 梁景霞, 陈美霞 , 等. 应用ISSR与SRAP分析烟草种质资源遗传多样性及遗传演化关系. 作物学报, 2012,38(8):1425-1434.
doi: 10.3724/SP.J.1006.2012.01425
[12] Denduang, Boripant J, Setaphan S ,et al. Determination of local tobacco cultivars using ISSR molecular marker. Chiang Mai Journal of Science, 2009,37(2):293-303.
[13] Bindler G, Plieske J, Bakaher N , et al. A high density genetic map of tobacco (Nicotiana tabacum L.) obtained from large scale microsatellite marker development. Theoretical & Applied Genetics, 2011,123(2):219-230.
doi: 10.1007/s00122-011-1578-8 pmid: 21461649
[14] Moon H S, Nicholson J S, Lewis R S . Use of transferable Nicotiana tabacum L. microsatellite markers for investigating genetic diversity in the genus Nicotiana. Genome, 2008,51(8):547-559.
doi: 10.1200/JCO.2005.05.511 pmid: 18650945
[15] 陈杰, 杨静, 陈建军 , 等. 烤烟种质资源形态学标记及SSR标记的多样性研究. 华南农业大学学报, 2017(4):450-457.
doi: 10.7671/j.issn.1001-411X.2013.04.002
[16] 丁浩, 郑唐春, 梁德洋 , 等. 一种简易高效提取多种植物纯净DNA的方法. 植物研究, 2015,35(3):457-461.
doi: 10.7525/j.issn.1673-5102.2015.03.021
[17] 童治军 . 烟草微卫星标记的开发与利用. 杭州:浙江大学, 2012.
[18] NY/T 1433-2007 Y/T 1433-2007. 水稻品种鉴定-DNA指纹方法. 北京: 中国标准出版社, 2007.
[19] NY/T 1432-2007 Y/T 1432-2007. 玉米品种鉴定-DNA指纹方法. 北京: 中国标准出版社, 2007.
[20] 宋海斌, 崔喜波, 马鸿艳 , 等. 基于SSR标记的甜瓜品种(系)DNA指纹图谱库的构建. 中国农业科学, 2012,45(13):2676-2689.
doi: 10.3864/j.issn.0578-1752.2012.13.012
[21] 刘国祥, 邹昆晏, 任民 , 等. 33份晒烟种质资源SSR标记的指纹图谱构建. 中国烟草学报, 2017,23(5):57-64.
doi: 10.16472/j.chinatobacco.2017.125
[22] 陈婷婷, 李显富, 贾春雷 , 等. 云烟87突变体新品系的特征特性及其DNA指纹图谱鉴定. 浙江大学学报(农业与生命科学版), 2016,42(2):179-189.
doi: 10.3785/j.issn.1008-9209.2015.05.041
[23] 李显富, 陈婷婷, 安雪琴 , 等. 烤烟K326突变体新品系‘片片黄’的性状表现及其DNA指纹图谱构建. 西南农业学报, 2015,28(3):1300-1305.
doi: 10.16213/j.cnki.scjas.2015.03.071
[24] 黄莉莎, 曹恒春, 王毅 , 等. 烟草主栽品种的SSR指纹图谱的构建及其应用. 山东农业大学学报(自然科学版), 2013,44(4):509-515.
[25] 徐军, 刘艳华, 任民 , 等. 普通烟草种质资源的SSR标记与指纹图谱分析. 中国烟草科学, 2011,32(2):62-65.
doi: 10.3969/j.issn.1007-5119.2011.02.014
[26] 陈世军, 张明泽, 姚玉仙 , 等. 基于SSR标记的黔南茶树种质资源DNA指纹图谱构建. 植物遗传资源学报, 2017,18(1):106-111.
doi: 10.13430/j.cnki.jpgr.2017.01.013
[27] 肖炳光, 卢江平, 卢秀萍 , 等. 烤烟品种的RAPD分析. 中国烟草学报, 2000,6(2):10-15.
[28] 杨柳, 汪斌, 童治军 , 等. 25份普通烟草种质资源遗传多样性的SSR标记分析. 福建农林大学学报(自然科学版), 2013,42(2):171-175.
doi: 10.3969/j.issn.1671-5470.2013.02.012
[29] 杨本超, 肖炳光, 陈学军 , 等. 基于ISSR标记的烤烟种质遗传多样性研究. 遗传, 2005,27(5):753-758.
doi: 10.3321/j.issn:0253-9772.2005.05.013
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