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作物杂志,2019, 第4期: 196–202 doi: 10.16035/j.issn.1001-7283.2019.04.030

• 植物保护 • 上一篇    

芝麻茎点枯病菌Macrophominaphaseolina纤维素降解酶活性分析

高树广1,2,徐博涵2,赵辉1,倪云霞1,李伟峰2,王瑞霞2,徐东阳2,杨光宇2,刘红彦1   

  1. 1 河南省农业科学院植物保护研究所,450002,河南郑州
    2 周口市农业科学院,466001,河南周口
  • 收稿日期:2019-03-11 修回日期:2019-07-10 出版日期:2019-08-15 发布日期:2019-08-06
  • 通讯作者: 刘红彦
  • 作者简介:高树广,助理研究员,从事芝麻抗病遗传和栽培技术研究
  • 基金资助:
    国家特色油料产业技术体系(CARS-14)

Analysis of Cellulase Activities of Macrophomina phaseolina in Sesame

Gao Shuguang1,2,Xu Bohan2,Zhao Hui1,Ni Yunxia1,Li Weifeng2,Wang Ruixia2,Xu Dongyang2,Yang Guangyu2,Liu Hongyan1   

  1. 1 Institute of Plant Protection, Henan Academy of Agricultural Sciences, Zhengzhou 450002, Henan, China
    2 Zhoukou Academy of Agricultural Sciences, Zhoukou 466001, Henan, China
  • Received:2019-03-11 Revised:2019-07-10 Online:2019-08-15 Published:2019-08-06
  • Contact: Hongyan Liu

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摘要：

测定芝麻茎点枯病菌（Macrophomina phaseolina）产生的纤维素降解酶的种类及活性大小,为进一步探讨其在致病过程中的作用奠定基础。从不同地区采集7株芝麻茎点枯病菌,液体培养提取粗酶液,采用分光光度法在540nm波长下测定离体条件下芝麻茎点枯病菌分泌的纤维素降解酶活性及变化趋势。结果表明:7个菌株均能检测到滤纸酶、天然纤维素降解酶、内切葡聚糖酶、外切葡聚糖酶和β-葡萄糖苷酶活性,酶活变化趋势表明不同采样时间酶活力大小不同,酶活变化趋势上都有峰值出现,但是不同菌株出现峰值的时间不同,酶活力综合活性大小差异极显著。说明芝麻茎点枯病菌能分泌一组胞外降解纤维素的酶系,并且该酶系能够降解芝麻秸秆纤维素,该结果为揭示芝麻茎点枯病菌对芝麻的致病机理提供理论依据。

关键词: 芝麻, 茎点枯病菌, 纤维素降解酶

Abstract:

To determine the types and activities of cellulases secreted by Macrophomina phaseolina and explore their roles in pathogenic process, seven strains of M. phaseolina were collected from different regions and studied. The crude enzyme solution was prepared by liquid culture. The activities and change graphs of cellulases in vitro were determined by spectrophotometry at 540nm wavelength. The results showed that the activities of filter paper enzyme, natural cellulase, endoglucanase, exoglucanase and β-glucosidase could be detected in all seven strains, and the enzyme activity of seven strains was different at different sampling time by observing the enzymatic activity curves. There were peaks on the enzyme activity curve but the time of peak value was different in different M. phaseolinas strains. In addition, there were significant differences in the comprehensive enzyme activities. It proved that M. phaseolina could secrete a group of extracellular cellulose and the cellulases could degrade cellulose of sesame straw. It provided an important theoretical basis to study the pathogenic mechanism of M. phaseolina in sesame.

Key words: Sesame, Macrophomina phaseolina, Cellulase

表1

供试菌株"

菌株
Strains		 采集年份
Year of collection		 采集地点
Location of collection
2010003 2010 江西省鄱阳县清湖村
Qinghu, Poyang, Jiangxi
2010010 2010 江苏省盱眙县龙泉村
Longquan, Xuyi, Jiangsu
2010028 2010 河南省唐河县赵庄村
Zhaozhuang, Tanghe, Henan
2010032 2010 湖北省襄樊钟岗服务区
Zhonggang Service Area, Xiangfan, Hubei
2010064 2010 河南省平舆县王栋桥庄
Wangdongqiao, Pingyu, Henan
2010082 2010 安徽省临泉县李窑村
Liyao, Linquan, Anhui
G243 2011 辽宁省朝阳县胜利乡
Shengli, Chaoyang, Liaoning

图1

葡萄糖标准曲线"

图2

芝麻茎点枯病菌滤纸酶活性"

图3

芝麻茎点枯病菌天然纤维素酶活性"

图4

芝麻茎点枯病菌内切葡聚糖酶活性"

图5

芝麻茎点枯病菌外切葡聚糖酶活性"

图6

芝麻茎点枯病菌β-葡萄糖苷酶活性"

表2

芝麻茎点枯病菌纤维素降解酶A值大小比较"

编号Number 2010003 2010010 2010028 2010032 2010064 2010082 G243
滤纸酶Filter paper enzyme 2.214Aa 0.838CDc 1.258Bb 0.973Cc 0.591Dd 2.112Aa 0.973Cc
天然纤维素酶Natural cellulase 2.407Aa 0.870DEde 1.611Cb 1.306Cc 0.707Ee 2.451Aa 1.074CDcd
内切葡聚糖苷酶Endoglucanase 3.614Bb 2.413De 3.017Cc 2.782Cd 2.017Ef 3.947Aa 2.801Ccd
外切葡聚糖苷酶Exoglucanase 0.436Aa 0.108Ccd 0.161Bb 0.140Cbc 0.049De 0.456Aa 0.101Cd
β-葡萄糖苷酶β-glucosidase 5.104Bb 0.379Dd 1.686Cc 0.932CDcd 0.313Dd 12.210Aa 0.626CDd
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