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作物杂志,2021, 第5期: 28–34 doi: 10.16035/j.issn.1001-7283.2021.05.005

• 遗传育种·种质资源·生物技术 • 上一篇    下一篇

玉米HSP90基因家族的全基因组鉴定与分析

曹丽茹(), 王国瑞(), 张新, 魏良明, 魏昕, 张前进, 邓亚洲, 王振华(), 鲁晓民()   

  1. 河南省农业科学院粮食作物研究所,450002,河南郑州
  • 收稿日期:2020-10-10 修回日期:2021-01-15 出版日期:2021-10-15 发布日期:2021-10-14
  • 通讯作者: 王振华,鲁晓民
  • 作者简介:曹丽茹,主要从事玉米遗产育种研究和生物信息数据分析,E-mail： caoliru008@126.com
  • 基金资助:
    河南省农业科学院杰出青年发展基金(2020JQ02)

Genome-Wide Identification and Analysis of HSP90 Gene Family in Maize

Cao Liru(), Wang Guorui(), Zhang Xin, Wei Liangming, Wei Xin, Zhang Qianjin, Deng Yazhou, Wang Zhenhua(), Lu Xiaomin()   

  1. Grain Crop Research Institute, Henan Academy of Agricultural Sciences, Zhengzhou 450002, Henan, China
  • Received:2020-10-10 Revised:2021-01-15 Online:2021-10-15 Published:2021-10-14
  • Contact: Wang Zhenhua,Lu Xiaomin

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摘要：

热激蛋白90（HSP90）广泛参与生物体的生长发育,且积极响应多种非生物胁迫。为全面解析玉米HSP90基因家族信息,对HSP90基因家族进化树、基因结构、保守基序（motif）、GO富集和组织表达模式等进行分析,并进一步分析ZmHSP90-1基因响应干旱胁迫的表达模式及蛋白互作关系。结果显示,从玉米全基因组水平共鉴定到11个ZmHSP90家族基因,被划分为5个亚族（I~V）;相邻进化树分支上的ZmHSP90基因具有相似的motif和基因结构;GO富集分析显示,11个ZmHSP90基因均参与了蛋白质折叠过程;表达模式分析显示,11个ZmHSP90基因在不同组织或器官中均有表达,其中ZmHSP90-1基因的表达量相对较高;ZmHSP90-1基因在耐旱性强的玉米自交系中具有更高的表达量,且积极响应干旱和复水过程;蛋白互作预测显示,ZmHSP90-1可能与其互作蛋白协同响应非生物胁迫,发挥生物学功能。

关键词: 玉米, 热激蛋白90, 表达水平, 干旱响应, 蛋白互作

Abstract:

Heat shock protein 90 (HSP90) are widely involved in the growth and development of organisms and actively responds to a variety of abiotic stresses. In order to analyze HSP90 gene family of maize, the phylogenetic tree gene structure, conserved motif, GO enrichment and tissue expression pattern of the family gene were analyzed, and further analyzed the expression pattern in response to drought stress and protein interaction relationship. The results showed that the eleven ZmHSP90 genes were identified from the whole genome level of maize and ZmHSP90 were divided into five groups (I-V). ZmHSP90 genes on adjacent phylogenetic tree branches had similar gene structure and motif. GO enrichment analysis showed that the eleven ZmHSP90 genes were involved in protein folding process. The expression pattern analysis showed that the eleven ZmHSP90 genes were expressed in different tissues or organs, and ZmHSP90-1 had relatively higher expression. ZmHSP90-1 gene had a higher expression level in drought-tolerant maize inbred lines, and responds to drought and rewater processes rapidly. Protein interaction prediction showed that ZmHSP90-1 might cooperate with its interacting protein to respond to abiotic stress, thus exerting its biological function.

Key words: Maize, HSP90, Expression level, Drought response, Protein interaction

表1

玉米HSP90基因家族

基因编号
Gene code		 基因ID
Gene ID		 染色体
Chromosome		 基因组位置
Genomic location		 内含子个数
Number of
introns		 开放阅读
框长度
ORF length(bp)		 氨基酸长度
Amino
acid length		 蛋白
分子量
MW (kDa)		 等电点
PI		 亚细胞定位预测
Subcellular
location prediction
ZmHSP90-1 Zm00001d006008 2 195179025~195182945 3 2097 698 80.72 4.73 细胞质
ZmHSP90-2 Zm00001d014792 5 63348326~63353481 15 2427 808 92.87 4.62 内质网、线粒体
ZmHSP90-3 Zm00001d020827 7 134001581~134006410 19 2394 797 90.20 4.63 内质网
ZmHSP90-4 Zm00001d020898 7 135842402~135846744 3 2100 699 80.27 4.75 细胞质
ZmHSP90-5 Zm00001d024903 10 93908317~93911235 2 2145 714 81.80 4.77 细胞质
ZmHSP90-6 Zm00001d031332 1 186376331~186380883 4 2028 675 77.38 4.73 细胞质
ZmHSP90-7 Zm00001d035285 6 19340069~19371142 20 2382 793 89.70 5.03 内质网
ZmHSP90-8 Zm00001d036401 6 86902776~86908235 15 2415 804 92.57 4.67 内质网、线粒体
ZmHSP90-9 Zm00001d041719 3 134955964~134966224 20 2442 813 91.18 4.96 内质网
ZmHSP90-10 Zm00001d052809 4 201661975~201666670 19 2376 791 89.05 4.78 内质网
ZmHSP90-11 Zm00001d052855 4 202671658~202675792 3 2100 699 80.36 4.69 细胞质

图1

玉米、拟南芥和高粱HSP90蛋白的进化树分析

图2

玉米HSP90家族成员的motif和基因结构分析 a图中不同颜色代表不同的motifs;b图中绿色代表UTR区域,黄色代表CDS区域

表2

玉米HSP90基因家族的GO分析

GO类型
GO type		 GO名称
GO ID		 GO途径
GO term		 基因编号
Gene code
生物进程 GO:0006457 蛋白质的折叠 ZmHSP90-1~11
Biological process GO:0034605 热响应 ZmHSP90-1、ZmHSP90-4、ZmHSP90-5、ZmHSP90-6、ZmHSP90-11
GO:0050821 蛋白质的稳定 ZmHSP90-1、ZmHSP90-4、ZmHSP90-5、ZmHSP90-6、ZmHSP90-11
GO:0009816 病菌的防御 ZmHSP90-5
GO:0061077 蛋白质的折叠 ZmHSP90-5
分子功能 GO:0005524 与ATP位点结合 ZmHSP90-1~11
Molecular function GO:0051082 与未知蛋白结合 ZmHSP90-1、ZmHSP90-3~11
GO:0000166 与核酸结合 ZmHSP90-5
细胞组分 GO:0005737 细胞质 ZmHSP90-1、ZmHSP90-4、ZmHSP90-5、ZmHSP90-6、ZmHSP90-11
Cellular component GO:0005829 胞质溶胶 ZmHSP90-1、ZmHSP90-4、ZmHSP90-5、ZmHSP90-6、ZmHSP90-11
GO:0005886 质膜 ZmHSP90-1、ZmHSP90-4、ZmHSP90-5、ZmHSP90-6、ZmHSP90-11
GO:0009986 细胞表面 ZmHSP90-1、ZmHSP90-4、ZmHSP90-5、ZmHSP90-6、ZmHSP90-11
GO:0032991 含蛋白质复合物 ZmHSP90-1、ZmHSP90-4、ZmHSP90-5、ZmHSP90-6、ZmHSP90-11
GO:0048471
细胞质区域
ZmHSP90-1、ZmHSP90-2、ZmHSP90-4、ZmHSP90-5、ZmHSP90-6、
ZmHSP90-8、ZmHSP90-11
GO:0009570 叶绿体基质 ZmHSP90-3、ZmHSP90-7、ZmHSP90-9、ZmHSP90-10
GO:0005783 内质网 ZmHSP90-8

图3

玉米HSP90家族基因在8个组织的表达谱

图4

ZmHSP90-1在干旱胁迫下的表达分析 M为抗旱型自交系郑8713,F为敏感型自交系郑K9713,Y指玉米幼叶片,J指玉米幼茎,G指玉米幼根,R1d和R3d分别表示复水1d和3d

表3

ZmHSP90-1功能互作蛋白预测

互作基因Interaction gene 基因描述Gene description 氨基酸长度Amino acid length 互作系数Interaction coefficient
GRMZM2G105019 SGT1疾病抗性蛋白同源物1 361 0.995
GRMZM2G149704 SGT1蛋白质同源物A 361 0.992
GRMZM2G329306 肽基脯氨酰顺反异构酶CYP40 389 0.979
GRMZM2G154312 伴侣蛋白SBA1 192 0.963
GRMZM2G038108 HSP蛋白ATPase的激活剂 446 0.958
GRMZM2G021816 HSP90蛋白ATPase的激活剂 348 0.958
GRMZM2G010944 蛋白质精氨酸N-甲基转移酶1.5 197 0.958
GRMZM5G868908 整合素β-1-结合蛋白2 225 0.953
GRMZM2G536120 锚蛋白重复家族蛋白 376 0.940
GRMZM2G155314 锚蛋白1 417 0.940
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