外源SLs和纳米K2MoO4对干旱胁迫下油菜种子萌发的影响

doi:10.16035/j.issn.1001-7283.2022.04.030

摘要/Abstract

摘要：

干旱胁迫是油菜种子萌发和生长发育的主要限制因子之一。以甘蓝型油菜品种Q2（抗旱型）和秦优8号（干旱敏感型）为试验材料,研究干旱对油菜种子萌发的影响,探讨外源生长调节物质独脚金内酯（SLs）和纳米材料（n-K₂MoO₄）对干旱胁迫下油菜种子萌发的生理调控作用。结果表明,干旱胁迫显著抑制种子萌发,SLs和n-K₂MoO₄均可显著提高发芽率,增加幼苗干重和子叶叶绿素含量,提高超氧化物歧化酶、过氧化物酶活性及可溶性糖、脯氨酸含量,降低丙二醛含量。0.10µmol/L GR24+0.24mmol/L K₂MoO₄对干旱胁迫下种子萌发和氧化损伤的缓解效应较好。外源SLs和n-K₂MoO₄通过提高油菜种子萌发期的保护酶活性和渗透调节物质含量来提高抗旱性。本研究表明植物激素和纳米材料在农业生产中具有应用价值。

关键词: 独脚金内酯, 纳米材料, 干旱胁迫, 种子萌发, 生理机制

Abstract:

Drought stress is one of the major abiotic constraints affecting rapeseed (Brassica napus L.) germination and growth. Using Q2 (drought-resistant) and Qinyou 8 (sensitive) as materials, the effects of drought on germination and the physiological mechanmism of seed germination under drought treated by exogenous growth regulators strigolactones (SLs) and nanomaterials (n-K₂MoO₄) were explored. The findings demonstrated that drought stress significantly inhibited seed germination, SLs and n-K₂MoO₄ significantly increased germination rate, seedling dry weight, and cotyledon chlorophyll content, as well as superoxide dismutase and peroxidase activities and soluble sugar, proline, and decreased malondialdehyde contents. In comparison to other treatments, 0.10μmol/L GR24 with 0.24mmol/L n-K₂MoO₄ exhibited stronger mitigation effects on seed germination and oxidative damage. According to this study, exogenous SLs and n-K₂MoO₄ could increase the activities of protective enzymes and the levels of osmotic regulators during rapeseed germination, which improved the ability to withstand drought. The results indicate plant hormones and nanomaterials might be used in agriculture.

Key words: Strigolactones, Nanomaterial, Drought stress, Seed germination, Physiological mechanism

庞星月, 万林, 李素, 王宇航, 刘晨, 肖晓璐, 李心昊, 马霓. 外源SLs和纳米K₂MoO₄对干旱胁迫下油菜种子萌发的影响[J]. 作物杂志, 2022 (4): 214–220

Pang Xingyue, Wan Lin, Li Su, Wang Yuhang, Liu Chen, Xiao Xiaolu, Li Xinhao, Ma Ni. Effects of Exogenous SLs and Nano-K₂MoO₄ on Seed Germination of Brassica napus L. under Drought Stress[J]. Crops, 2022(4): 214–220

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品种 Variety	处理 Treatment	GR (%)	GI	MT	VI	出苗率 Rate of emergence (%)
Q2	CK1	100.00±0.00a	58.82±1.15a	1.10±0.09e	515.82±35.92b	97.78±3.85a
	CK2	98.89±0.96a	30.88±0.70f	3.30±0.11b	51.46±7.43f	35.56±10.18d
	T1	100.00±0.00a	53.18±1.60bc	1.48±0.13de	686.09±13.22a	73.33±2.89b
	T2	100.00±0.00a	36.80±0.19e	2.73±0.05c	257.13±10.51d	62.22±11.10bc
	T3	100.00±0.00a	51.25±1.25cd	1.61±0.11d	189.78±4.88e	76.11±10.72b
秦优8号	CK1	100.00±0.00a	55.40±2.92ab	1.32±0.21de	454.05±43.46c	96.67±4.41a
Qinyou 8	CK2	40.67±16.17b	8.06±1.84g	6.64±0.85a	16.55±5.95f	16.67±6.67e
	T1	98.89±1.93a	39.77±0.49e	2.58±0.05c	456.9±45.99c	34.44±2.55d
	T2	98.61±0.00a	31.38±3.98f	3.04±0.13bc	147.65±1.84e	44.44±12.95 d
	T3	98.89±1.93a	49.39±1.40d	1.72±0.17d	186.54±57.76e	49.44±13.98cd

品种 Variety	处理 Treatment	干重（mg/株） Dry weight (mg/plant)	主根长（cm/株） Main root length (cm/plant)	侧根长（cm/株） Lateral root length (cm/plant)	侧根数 Root hair number
Q2	CK1	30.90±1.59cd	7.40±0.72c	7.09±1.41a	8.00±1.31b
	CK2	25.47±5.18ef	1.40±0.18g	1.12±0.40ef	2.00±0.36c
	T1	37.46±2.75a	12.24±0.43a	3.17±0.85bc	10.00±2.31a
	T2	36.93±1.53a	6.15±0.47d	2.12±1.49cde	3.00±1.22c
	T3	35.57±1.40ab	2.96±0.07ef	1.27±0.21def	4.00±0.58c
秦优8号	CK1	26.97±0.90de	7.08±0.50cd	4.19±0.26b	7.00±1.67b
Qinyou 8	CK2	21.40±1.82f	2.05±0.41fg	0.32±0.07f	1.00±0.23c
	T1	27.30±1.70de	10.87±0.77b	3.59±0.76bc	11.00±0.67a
	T2	32.33±1.60bc	3.63±0.45e	2.40±0.40cd	3.00±0.50c
	T3	31.10±3.30cd	3.22±1.37e	1.23±0.35def	3.00±0.58c

处理 Treatment	Chl a		Chl b		Chl a+b		Chl a/Chl b
处理 Treatment	Q2	秦优8号 Qinyou 8	Q2	秦优8号 Qinyou 8	Q2	秦优8号 Qinyou 8	Q2	秦优8号 Qinyou 8
CK1	8.93±0.32bc	6.78±0.66e	4.40±0.19abc	3.97±0.36bce	13.33±0.49bc	10.75±1.02d	2.03±0.04bc	1.71±0.01d
CK2	7.22±0.31e	5.47±0.37f	3.84±0.19cef	3.31±0.24f	11.06±0.37d	8.78±0.60e	1.88±0.12d	1.65±0.03e
T1	8.28±0.12cd	6.89±0.60e	4.27±0.19abc	4.10±0.29bce	12.55±0.31c	10.98±0.39d	1.94±0.06c	1.69±0.27d
T2	10.04±0.59a	7.96±0.29d	4.73±0.20a	4.77±0.88a	14.77±0.79a	12.72±0.59c	2.12±0.05a	1.71±0.35c
T3	9.45±0.42ab	5.93±0.32f	4.53±0.14ab	3.58±0.08ef	13.98±0.57ab	9.52±0.40e	2.08±0.03ab	1.65±0.06e

指标Index	GR	GI	VI	MT	DW	LRL	MRL	Chl	SOD	POD	MDA	H₂O₂	O^-₂·	Pro	SS
GR	1
GI	0.731^**
VI	0.462^*	0.683^**
MT	-0.836^**	-0.965^**	-0.626^**
DW	0.591^**	0.428^*	0.455^*	-0.514^**
LRL	0.067	0.438^*	0.650^**	-0.303	-0.066
MRL	0.211	0.357	0.878^**	-0.330	0.227	0.561^**
Chl	0.543^**	0.334	0.339	-0.389	0.727^**	0.115	0.183
SOD	0.201	-0.027	-0.124	-0.064	0.470^*	-0.585^**	-0.177	0.108
POD	0.287	0.154	-0.056	-0.198	0.519^**	-0.489^**	-0.154	0.080	0.744^**
MDA	-0.817^**	-0.925^**	-0.530^**	0.939^**	-0.511^**	-0.361	-0.169	-0.506^**	-0.089	-0.169
H₂O₂	-0.629^**	-0.862^**	-0.720^**	0.844^**	-0.478^**	-0.658^**	-0.417^*	-0.486^**	0.126	-0.027	0.883^**
O^-₂·	-0.889^**	-0.890^**	-0.645^**	0.935^**	-0.672^**	-0.322	-0.358	-0.670^**	-0.105	-0.232	0.927^**	0.852^**
Pro	0.308	0.062	-0.136	-0.146	0.576^**	-0.596^**	-0.234	0.320	0.909^**	0.789^**	-0.188	0.081	-0.260
SS	0.181	-0.130	-0.185	0.040	0.453^*	-0.647^**	-0.165	0.280	0.887^**	0.562^**	-0.023	0.195	-0.115	0.891^**	1

外源SLs和纳米K₂MoO₄对干旱胁迫下油菜种子萌发的影响

Effects of Exogenous SLs and Nano-K₂MoO₄ on Seed Germination of Brassica napus L. under Drought Stress

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