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作物杂志,2023, 第5期: 98–103 doi: 10.16035/j.issn.1001-7283.2023.05.014

• 生理生化·植物营养·栽培耕作 • 上一篇    下一篇

黑暗和强光下脱水对小麦离体叶片光系统活性的影响

杨程1(), 张德奇1(), 杜思梦1, 张丽佳2, 靳海洋1, 李滢1, 邵运辉1, 王汉芳1, 方保停1, 李向东1(), 刘美君2()   

  1. 1河南省农业科学院小麦研究所/河南省小麦产量—品质协同提升工程研究中心/郑州大学研究生培养基地/农业农村部中原地区作物栽培科学观测实验站/河南省小麦生物学重点实验室，450002，河南郑州
    2新疆农业大学草业学院，830052，新疆乌鲁木齐
  • 收稿日期:2022-03-29 修回日期:2022-04-06 出版日期:2023-10-15 发布日期:2023-10-16
  • 通讯作者: 李向东，主要从事小麦栽培与耕作研究，E-mail：hnlxd@126.com; 刘美君为共同通信作者，主要从事植物逆境生理研究，E-mail：lebaby7@163.com
  • 作者简介:杨程，主要从事作物逆境生理与调控研究，E-mail：luckytiger.com@163.com;|张德奇为共同第一作者，主要从事小麦栽培与耕作研究，E-mail：dqzhang3@163.com
  • 基金资助:
    国家重点研发计划(2022YFD2300202);河南省现代农业产业技术体系建设专项(HARS-22-01-G5);中原科技创新领军人才计划资助项目(224200510028);河南省农业科学院科技创新团队

Effects of Dark and Strong Light Dehydration on the Photosystem Activity in Wheat Leaves in Vitro

Yang Cheng1(), Zhang Deqi1(), Du Simeng1, Zhang Lijia2, Jin Haiyang1, Li Ying1, Shao Yunhui1, Wang Hanfang1, Fang Baoting1, Li Xiangdong1(), Liu Meijun2()   

  1. 1Wheat Research Institute, Henan Academy of Agricultural Sciences/Henan Engineering Research Center for Wheat Yield-Quality Simultaneous Improvement/Zhengzhou University Graduate Training Base/Scientific Observing and Experimental Station of Crop Cultivation in Central Plains, Ministry of Agriculture and Rural Affairs/Henan Provincial Key Laboratory of Wheat Biology, Zhengzhou 450002, Henan, China
    2College of Grassland Science, Xinjiang Agricultural University, Urumqi 830052, Xinjiang, China
  • Received:2022-03-29 Revised:2022-04-06 Online:2023-10-15 Published:2023-10-16

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摘要：

脱水是小麦叶片在干旱条件下的自然失水过程，为了探明黑暗和强光下脱水对小麦叶片光系统的影响机制及其差异，采用叶绿素荧光动力学技术，分析了黑暗和强光下脱水不同时间对小麦离体叶片光系统活性的影响。结果表明，强光加速了叶片脱水过程，黑暗脱水条件下，叶片的最大光化学效率随着脱水程度的增加并没有出现明显下降，而强光下显著下降，且随着脱水程度加剧而下降速度逐渐加快;强光脱水条件下，QA到QB电子传递受到抑制，且抑制程度大于黑暗脱水;无论是黑暗还是强光脱水条件，光系统II（PSII）供体侧活性均没有显著变化;PSII单元间的偶联程度在黑暗条件下没有明显变化，在强光脱水条件下则大幅下降。最终表明，脱水导致的PSII活性和光合性能的下降依赖于光照，强光下随着脱水程度的加剧，PSII对光能的吸收和传递活性以及PSII单元间能量偶联程度逐渐下降，而在黑暗条件下，仅严重脱水时才会导致最大光化学效率、反应中心数目以及QA到QB电子传递活性的小幅下降。

关键词: 小麦, 脱水, 干旱, 光合作用, 光系统II

Abstract:

Dehydration is the natural process of wheat leaves under drought conditions. In order to explore the mechanism of dark and strong light dehydration on photosynthetic mechanism and their differences in detached wheat leaves, the effects of dehydration with dark and strong light on the activity of wheat photosystem were determined and analyzed by using chlorophyll fluorescence dynamics technology. The results showed that the strong light accelerated the leaf dehydration process. Under the condition of dark dehydration, the maximum photochemical efficiency did not decrease significantly with the increase of dehydration degree, but decreased significantly under strong light, and the decline rate gradually accelerated with the increase of dehydration degree. Under the condition of strong light dehydration, the electron transfer from QA to QB was inhibited, and the degree of inhibition was greater than that of dark dehydration. There was no significant change in photosystem II (PSII) donor side activity under either dark or strong light dehydration conditions. The coupling degree between PSII units did not change significantly under dark conditions, but decreased significantly under strong light dehydration. Finally, it showed that the decline of PSII activity and photosynthetic performance caused by dehydration depended on light, under strong light, with the increase of dehydration degree, the absorption and transfer activity of PSII to light energy and the degree of energy coupling between PSII units gradually decline, while under dark conditions, only severe dehydration will lead to the maximum photochemical efficiency, the number of reaction centers and the electron transfer activity from QA to QB decreased slightly.

Key words: Wheat, Dehydration, Drought, Photosynthesis, Photosystem II

表1

叶绿素快速荧光诱导动力学曲线参数及公式

参数Parameter 计算方法Method of calculation
FM 暗适应后照光获得的最大荧光强度
FO 叶绿素荧光诱导动力学曲线20μs的荧光强度
Fv Fv=FM?FO
Ft t时的荧光强度F
FK K点（0.3ms）的荧光强度
FJ J点（3ms）的荧光强度
FI I点（30ms）的荧光强度
VJ VJ=(FJ?FO)/(FM?FO)
VI VI=(FI ?FO)/(FM ?FO)
MO MO=4(F300μs?FO)/(FM?FO)
ΨO ψO=1?VJ
φPO φPO=Fv/FM=(FM?FO)/FM
φEO φEo=ETo/ABS=[1?(FO/FM)]?ψO
φDO φDO=1?φPO
φRO φRO=φPO×(1?VI)
σRO σRO=(1?VI)/(1?VJ)
ABS/RC ABS/RC=MO?(1/ VJ)?(1/φPO)
RC/CSm RC/CSm=φPO×(VJ/MO)×FM
PIABS PIABS=(RC/ABS)×[φPO/(1?φPO)]×[ψO/(1?ψO)]

图1

强光和黑暗脱水不同时间下小麦叶片的相对含水量

图2

黑暗和强光脱水不同时间小麦叶片的OJIP曲线

图3

黑暗和强光下脱水不同时间对OJIP曲线O-J段荧光的影响

图4

黑暗和强光处理不同时间对OJIP曲线O-K段荧光的影响

图5

黑暗和强光处理下不同相对含水量叶片荧光参数的变化

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