谷子矮秆突变体d93090的表型变异及其对赤霉素的敏感性分析

doi:10.16035/j.issn.1001-7283.2019.06.005

作物杂志,2019, 第6期: 27–32 doi: 10.16035/j.issn.1001-7283.2019.06.005

• 遗传育种·种质资源·生物技术 • 上一篇下一篇

谷子矮秆突变体d93090的表型变异及其对赤霉素的敏感性分析

赵丽娟¹,袁红梅²,赵丽伟³,郭文栋⁴,李志江¹,李祥羽¹,马金丰¹,李延东¹,宋维富¹,杨雪峰¹,刘东军¹

¹黑龙江省农业科学院作物资源研究所,150086,黑龙江哈尔滨
²黑龙江省农业科学院经济作物研究所,150086,黑龙江哈尔滨
³哈尔滨市阿城区杨树镇政府农业技术综合服务中心,150314,黑龙江哈尔滨
⁴黑龙江省科学院自然与生态研究所,150040,黑龙江哈尔滨

收稿日期:2019-05-17 修回日期:2019-10-12 出版日期:2019-12-15 发布日期:2019-12-11
作者简介:赵丽娟,副研究员,主要从事作物资源与遗传育种研究
基金资助:
黑龙江省自然科学基金面上项目(C201316);哈尔滨市科技局青年后备人才项目(2014RFQYJ138)

The Phenotypic Variations and GA Sensitivity of a Dwarf Mutant d93090 in Foxtail Millet

Zhao Lijuan¹,Yuan Hongmei²,Zhao Liwei³,Guo Wendong⁴,Li Zhijiang¹,Li Xiangyu¹,Ma Jinfeng¹,Li Yandong¹,Song Weifu¹,Yang Xuefeng¹,Liu Dongjun¹

¹Crop Resources Institute, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, Heilongjiang,China
²Industrial Crops Institute, Heilongjiang Academy of Agricultural Sciences, Harbin 150086,Heilongjiang, China
³Comprehensive Agricultural Technology Service Center, Yangshu Town Government of Acheng District, Harbin 150314, Heilongjiang, China
⁴Nature and Ecology Institute, Heilongjiang Academy of Sciences, Harbin 150040, Heilongjiang,China

Received:2019-05-17 Revised:2019-10-12 Online:2019-12-15 Published:2019-12-11

摘要/Abstract

摘要：

谷子矮秆突变体93090（暂命名为d93090）是野生型高秆谷子品系93090经 ⁶⁰Co-γ辐射诱变获得的,本研究对其矮化表型及其对赤霉素的敏感性进行了分析。结果表明,d93090株高约为野生型的60%左右,叶色变深、茎秆稍倾斜、茎节数不变、花期较对照推迟3~5d;幼苗期的苗长、第二叶鞘长和胚轴长均对外源GA₃敏感,拔节期喷施外源GA₃,d93090的株高部分恢复;d93090内源GA_l含量显著低于野生型。d93090突变体是个半矮秆的突变类型,为谷子矮化育种提供了新材料,其矮秆性与GA生物合成途径相关。

关键词: 谷子, 半矮秆突变体, 表型, GA₃敏感性

Abstract:

Abstact A stably inherited dwarf mutant d93090 was obtained from a wild type tall line 93090 in foxtail millet induced by ⁶⁰Co-γ irradiation. Then its dwarfing morphology and the characteristics response to gibberellin were analyzed. The results showed: Compared with the wild type, the plant height of the dwarf mutant was about 60% of that of the wild type. And other features, such as darker leaves in color, slightly sloping stem, the same stem nodes and delayed flowering time of about 3-5 days were also observed. The seedling length, the length of second leaf sheath and hypocotyl length at seedling stage were sensitive to exogenous GA₃. While exogenous GA₃ was sprayed at jointing stage, plant height of dwarf mutant was partially restored. The content of endogenous GA_l in dwarf mutant was significantly lower than that in wild type. It can be concluded this mutant is a semi-dwarf mutant and is related to the GA metabolic pathway. This study provides new germplasm for dwarf breeding in foxtail millet.

Key words: Foxtail millet, Semi-dwarf mutant, Phenotype, GA₃ sensitivity

赵丽娟,袁红梅,赵丽伟,郭文栋,李志江,李祥羽,马金丰,李延东,宋维富,杨雪峰,刘东军. 谷子矮秆突变体d93090的表型变异及其对赤霉素的敏感性分析[J]. 作物杂志, 2019 (6): 27–32

Zhao Lijuan,Yuan Hongmei,Zhao Liwei,Guo Wendong,Li Zhijiang,Li Xiangyu,Ma Jinfeng,Li Yandong,Song Weifu,Yang Xuefeng,Liu Dongjun. The Phenotypic Variations and GA Sensitivity of a Dwarf Mutant d93090 in Foxtail Millet[J]. Crops, 2019(6): 27–32

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材料 Meterial	株高 Plant height	叶片数 Leaf number	第4叶长 4th leaf length	第5叶长 5th leaf length	第6叶长 6th leaf length	第4叶宽 4th leaf width	第5叶宽 5th leaf width	第6叶宽 6th leaf width
WT	44.5±4.8	11.9±0.6	49.3±2.4	45.5±1.7	39.7±3.2	2.32±0.2	2.18±0.2	2.03±0.1
d93090	33.4±2.2^**	11.4±0.5	46.0±2.0^**	42.4±1.3^**	36.4±1.8^**	2.33±0.1	2.20±0.1	2.00±0.1

材料 Meterial	株高 Plant height	叶片数 Leaf number	第1叶长 1st leaf length	第2叶长 2nd leaf length	第3叶长 3rd leaf length	第2叶宽 2nd leaf width	第3叶宽 3rd leaf width
WT	175.5±4.50	13.2±0.40	39.20±2.60	41.85±2.30	44.35±2.40	2.78±0.20	2.57±0.05
d93090	107.0±1.60^**	12.8±0.60	40.17±2.00	45.36±2.20^**	47.30±1.40^**	2.95±0.01	2.77±0.10

材料 Meterial	株高(cm) Plant height	穗长(cm) Panicle length	穗粗(cm) Panicle diameter	穗重(g) Panicle weight	穗粒重(g) Grain weight per panicle	千粒重(g) 1000- seed weight
WT	176.05±4.4	18.5±1.4	2.4±0.2	20.9±3.4	18.45±3.1	1.67±0.1
d93090	107.25±1.7^**	24.1±0.8^**	2.3±0.2	20.2±2.2	17.20±2.0	1.67±0.1

谷子矮秆突变体d93090的表型变异及其对赤霉素的敏感性分析

The Phenotypic Variations and GA Sensitivity of a Dwarf Mutant d93090 in Foxtail Millet

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