SiASRs家族基因的鉴定及表达分析

doi:10.16035/j.issn.1001-7283.2019.06.006

摘要/Abstract

摘要：

ASR蛋白是植物特有的一类蛋白,是参与并提高植物抗旱性和耐盐性过程中非常重要的蛋白之一。利用生物信息学方法,对8个谷子SiASRs家族基因编码的蛋白进行理化性质的分析、启动子的分析和系统进化树构建等,并结合qRT-PCR进行10% PEG-6000和150mmol/L NaCl胁迫下的表达分析。结果表明,谷子SiASRs家族的8个基因编码的蛋白都包含典型的ABA/WDS结构,该基因家族编码的蛋白质都不具有信号肽。系统发育树分析结果表明,在遗传进化上谷子与柳枝稷亲缘关系较近,与双子叶的番茄和大豆ASR蛋白的亲缘关系较远。PEG胁迫下基因的表达分析显示,8个SiASRs基因在受到PEG胁迫诱导后在根、茎、叶中表达量差异显著。NaCl胁迫下基因的表达分析结果显示,8个ASR基因对NaCl胁迫的响应主要在叶中体现,整体呈上调表达趋势;8个ASR基因在茎中表达变化较为稳定;在根部主要由ASR1、ASR2、ASR4、ASR5、ASR6参与NaCl胁迫响应,均为上调表达。

关键词: 谷子, ASR, 家族基因, 生物信息学, 表达分析

Abstract:

ASR protein is a plant-specific protein, which it is one of the most important proteins involved in the drought resistance and salt tolerance of plants. Bioinformatics methods were used to analyze the physicochemical properties of proteins, promoter analysis and phylogenetic tree construction of eight SiASRs family genes in millet. PEG-6000 (10%) and salt (150mmol/L) stresses were arranged for study expression using qRT-PCR. The proteins encoded by the 8 millet SiASRs family genes contained typical ABA/WDS structures, and no signal peptides. Phylogenetic tree showed that the genetic relationship between millet and switchgrass was closer. The evolution among millet ASR protein and tomato ASR protein and soybean ASR protein was relatively far. The results of expression analysis under PEG stress showed that the expression of 8 SiASR was significantly different in different tissues after induction by drought stress. The results of expression analysis under salt stress showed that the responses of 8 ASR genes to salt stress were mainly reflected in the leaves, and the overall expression was up-regulated. The expression of 8 ASR genes in stems was stable; In the roots, ASR1, ASR2, ASR4, ASR5 and ASR6 were involved in salt stress response, and all 5 genes were up-regulated.

Key words: Setaria italica, ASR, Family gene, Bioinformatics, Expression analysis

宋健,晓宁,王海岗,陈凌,王君杰,刘思辰,乔治军. SiASRs家族基因的鉴定及表达分析[J]. 作物杂志, 2019 (6): 33–42

Song Jian,Cao Xiaoning,Wang Haigang,Chen Ling,Wang Junjie,Liu Sichen,Qiao Zhijun. Identification and Expression Analysis of ASR Family Genes in Setaria italica[J]. Crops, 2019(6): 33–42

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基因名称 Gene name	基因ID Gene ID	基因长度(bp) Length of gene	编码区(bp)Length of CDS	氨基酸数目(aa) Number of amino acids	基因所在染色体的位置 Location of the genes on the chromosome	等电点 PI	分子量(kDa) Molecular weight	亲水性指数 Hydrophilic index
SiASR1	Seita.1G187200	876	324	107	scaffold_1：26937730..26938605 reverse	6.76	11.82	-1.190
SiASR2	Seita.5G463600	1 020	522	173	scaffold_5：46902215..46903234 forward	6.26	19.38	-1.277
SiASR3	Seita.5G463700	1 167	318	105	scaffold_5：46906469..46907635 reverse	9.73	11.71	-1.147
SiASR4	Seita.5G463800	590	309	103	scaffold_5：46907284..46907873 forward	9.80	11.50	-1.417
SiASR5	Seita.7G097100	803	306	101	scaffold_7：19994321..19995123 reverse	6.81	11.48	-1.366
SiASR6	Seita.7G097200	1 318	735	244	scaffold_7：19998219..19999536 reverse	5.03	26.00	-1.636
SiASR7	Seita.7G291500	984	417	138	scaffold_7：33499331..33500314 forward	5.88	15.45	-1.298
SiASR8	Seita.8G045700	917	414	137	scaffold_8：3645891..3646807 reverse	6.15	15.42	-1.346

基序名称 Motif name	基序宽度 Motif width	基序的氨基酸组成 Amino acid composition of the motif
motif1	50	QLGELGAVAAGAYALYEKHKAKKDPEHAHRHKIKEEVAAAAAVGSGGYAF
motif2	14	EDYKKEEKEHKHKE
motif3	11	HEHHEKKEAKK
motif4	18	MAEEKHHHHYFHHHKDED
motif5	6	QQPAGG
motif6	6	SGTDEC
motif7	8	TVAEEVVT
motif8	6	DDCYNG
motif9	8	RVGAGGYC
motif10	6	KHHHLF
motif11	6	HGSRRD
motif12	7	VGRRGGG
motif13	11	RNRAVGDDEYN
motif14	6	KKHHFF
motif15	6	DDEKNK
motif16	6	YGGGYN
motif17	7	YGRGGGD
motif18	6	HRRHGH