富氢水对干旱胁迫下大麦种子萌发及幼苗生物量分配的影响

doi:10.16035/j.issn.1001-7283.2021.04.032

摘要/Abstract

摘要：

为探讨富氢水对干旱胁迫下大麦种子萌发期抗旱性与幼苗生物量分配的影响,以大麦品种“新啤6号”为试材,采用20%聚乙二醇（PEG）-6000模拟干旱胁迫,比较不同浓度富氢水浸种处理后大麦种子的发芽率和幼苗根冠比等16个指标的差异。结果表明,适宜浓度的富氢水处理能显著提高干旱胁迫下大麦种子的发芽率、干物质转移量、转移率及转化效率,并减少呼吸消耗干物质量,还可以降低干旱胁迫下大麦幼苗根冠比,增加幼苗根和芽的干重,促进幼苗可溶性糖、可溶性蛋白及叶绿素累积。由此可知,一定浓度的富氢水能通过调控种子干物质转运的途径提升干旱胁迫下大麦种子的萌发率,并可通过调节可溶性糖、可溶性蛋白和叶绿素含量降低干旱胁迫对大麦幼苗根和芽生物量分配的不利影响。

关键词: 富氢水, 干旱胁迫, 大麦, 萌发

Abstract:

To investigate the effects of hydrogen-rich water (HRW) on drought resistance in germination period and seedling biomass distribution under drought stress, barley variety Xinpi 6 was used as material, which cultured in 20% polyethylene glycol-6000 (PEG). The 16 indexes were compared in different concentrations of HRW, such as germination rate and seedling root-shoot ratio. The results showed that the suitable concentration of HRW treatment could significantly increase the germination rate, dry matter transfer amount, transfer rate, and conversion efficiency; reduced respiratory consumption of dry matter and root-shoot ratio; increased the roots and buds’ dry weight; promoted the accumulation of soluble sugar, soluble protein, and chlorophyll in barley seedlings. It could be concluded that suitable concentration of HRW could increase the germination rate of barley seeds under drought stress by regulating the transport of dry matter, and reduce the adverse effects of drought stress on the proportion of root and bud biomass of seedlings by regulating the contents of soluble sugar, soluble protein, and chlorophyll.

Key words: Hydrogen-rich water, Drought stress, Barley, Germination

宋瑞娇, 冯彩军, 齐军仓. 富氢水对干旱胁迫下大麦种子萌发及幼苗生物量分配的影响[J]. 作物杂志, 2021 (4): 206–211

Song Ruijiao, Feng Caijun, Qi Juncang. Effects of Hydrogen-Rich Water on Barley Seed Germination and Barley Seedling Biomass Distribution under Drought Stress[J]. Crops, 2021(4): 206–211

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参考文献 24

[1]	刘慧, 薛凤蕊. 中国大麦贸易现状及发展趋势. 农业展望, 2015,11(8):66-69.
[2]	周曙东, 周文魁, 林光华, 等. 未来气候变化对我国粮食安全的影响. 南京农业大学学报(社会科学版), 2013,13(1):56-65.
[3]	Tan L Y, Chen S X, Wang T, et al. Proteomic insights into seed germination in response to environmental factors. Proteomics, 2013,13(12/13):1850-1870.
[4]	李淑梅, 董丽平, 王付娟. PEG模拟干旱胁迫对大麦种子萌发及生理特性的响应. 种子, 2016,35(10):99-101.
[5]	包奇军, 潘永东, 张华瑜, 等. 旱胁迫对啤酒大麦产量及酿造品质的影响. 干旱地区农业研究, 2016,34(5):27-34.
[6]	宋瑞娇, 齐军仓. 氢气的植物学作用研究进展. 植物生理学报, 2020,56(5):913-920.
[7]	Xie Y, Mao Y, Zhang W, et al. Reactive oxygen species-dependent nitric oxide production contributes to hydrogen-promoted stomatal closure in Arabidopsis. Plant Physiology, 2014,165(2):759-773.
[8]	Chen Y, Wang M, Hu L, et al. Carbon monoxide is involved in hydrogen gas-induced adventitious root development in cucumber under simulated drought stress. Frontiers in Plant Science, 2017,8:128.
[9]	Felix K, Su J C, Lu R F, et al. Hydrogen-induced tolerance against osmotic stress in alfalfa seedlings involves ABA signaling. Plant Soil, 2019,445:409-423.
[10]	Tomoki S, Ryosuke K, Bunpei S. A convenient method for determining the concentration of hydrogen in water:use of methylene blue with colloidal platinum. Medical Gas Research, 2012,2(1):1-6.
[11]	Soltani A, Gholipoor A, Zeinali E. Seed reserve utilization and seedling growth of wheat as affected by drought and salinity. Environmental and Experimental Botany, 2006,55:195-200.
[12]	李合生, 孙群, 赵世杰. 植物生理生化实验原理和技术. 北京: 高等教育出版社, 2000: 195-197.
[13]	Bradford M M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 1976,72:248-254.
[14]	Arnon D I. Copper enzymes in isolated chlorplasts polyphenoloxidase in Beta vulgaris. Plant Physiology, 1949,24:1-15.
[15]	Xu S, Zhu S, Jiang Y, et al. Hydrogen-rich water alleviates salt stress in rice during seed germination. Plant and Soil, 2013,370:47-57.
[16]	田婧芸, 张慧洁, 陕嘉楠, 等. 富氢水处理对铜胁迫下小麦幼苗生长及其细胞结构的影响. 河南农业大学学报, 2018,52(2):193-198.
[17]	施成晓, 陈婷, 王昌江, 等. 干旱胁迫对不同抗旱性小麦种子萌发及幼苗根芽生物量分配的影响. 麦类作物学报, 2016,36(4):483-490.
[18]	贺海波, 李彦. 干旱、盐胁迫条件下两种盐生植物生物量分配对策的研究. 干旱区研究, 2008,25(2):242-247.
[19]	代小冬, 徐心志, 朱灿灿, 等. 谷子苗期对不同程度干旱胁迫的响应及抗旱性评价. 作物杂志, 2016(1):140-143.
[20]	姜颖, 左官强, 王晓楠, 等. 烯效唑浸种对干旱胁迫下工业大麻幼苗形态、渗透调节物质及内源激素的影响. 干旱地区农业研究, 2020,38(3):74-80.
[21]	侯俊峰, 黄鑫, 侯阁阁, 等. 非结构性碳水化合物积累与小麦植株抗旱性及产量的关系. 西北农业学报, 2017,26(11):1590-1597.
[22]	Wang Y, Duan X L, Xu S, et al. Linking hydrogen-mediated boron toxicity tolerance with improvement of root elongation,water status and reactive oxygen species balance:a case study for rice. Annals of Botany, 2016,118(7):1279-1291.
[23]	顾正中, 周羊梅, 杨子博, 等. 干旱胁迫下淮麦33等不同小麦品种幼苗生理响应的研究. 西南农业学报, 2017,30(1):67-71.
[24]	刘军, 齐广平, 康燕霞, 等. 不同灌溉处理下紫花苜蓿光合特性、叶绿素荧光参数及生物量的变化. 草地学报, 2019,27(6):1569-1576.

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富氢水浓度 HRW concentration (%)	干物质转移量 Dry matter transfer amount (mg)	干物质转移率 Dry matter transfer rate (%)	呼吸消耗干物质量 Respiratory consumption of dry matter (mg)	干物质转化效率 Dry matter conversion efficiency (%)
0(CK)	426.39±20.15bA	67.72±3.20bA	313.79±22.98aA	26.59±1.98bBC
25	454.13±8.20abA	72.12±1.31abA	302.89±12.83bA	33.36±1.64aB
50	465.49±19.97abA	73.93±3.17abA	303.79±20.49bA	34.87±1.58aA
75	474.26±2.20aA	75.32±0.35aA	310.89±8.68bA	34.45±1.61aB
100	482.59±10.51aA	76.64±1.67aA	362.93±15.91aA	24.87±1.73aC

富氢水浓度 HRW concentration (%)	幼苗干重 Seedling dry weight (mg)	根干重 Root dry weight (mg)	芽干重 Bud dry weight (mg)	根冠比 Root-shoot ratio
0(CK)	112.60±3.10bB	72.10±2.48cC	40.50±2.35cB	1.79±0.14abAB
25	151.23±4.72aA	82.70±4.69bcBC	68.53±0.68aA	1.21±0.07cB
50	161.70±1.76aA	91.97±2.40bAB	69.73±2.11aA	1.32±0.07bcAB
75	163.37±7.15aA	105.87±6.95aAB	57.50±1.81bA	1.85±0.13abAB
100	119.67±5.82bB	79.80±0.81bcBC	39.87±6.54cB	2.10±0.31aA

富氢水浓度 HRW concentration (%)	叶绿素a Chlorophyll a	叶绿素b Chlorophyll b	叶绿素总含量 Total chlorophyll content
0(CK)	780.07±23.21dC	249.68±21.52abA	1029.76±33.64cD
25	1173.08±63.89aA	259.51±15.37aA	1432.59±78.98aA
50	1034.44±34.40bB	218.03±7.17bA	1252.48±41.51bB
75	968.96±8.45bB	214.88±2.79bA	1183.84±9.79bBC
100	850.46±33.16cC	219.22±34.81bA	1069.69±67.54cCD