作物杂志,2025, 第4期: 2940 doi: 10.16035/j.issn.1001-7283.2025.04.004
王丹1(), 张志成2,3(
), 韩海霞1, 韩娜1, 王春勇4, 王正1, 潘孟洋1, 李强5, 王懿茜2, 刘宇飞2, 张丹2
Wang Dan1(), Zhang Zhicheng2,3(
), Han Haixia1, Han Na1, Wang Chunyong4, Wang Zheng1, Pan Mengyang1, Li Qiang5, Wang Yiqian2, Liu Yufei2, Zhang Dan2
摘要:
为明确马铃薯种质资源的亲缘关系,利用SSR分子标记对63份马铃薯种质资源的遗传多样性进行分析。结果显示,共扩增到164个多态性条带,其中多态性位点145个,平均条带数为5.13个。有效等位基因数、Nei’s遗传多样性指数和Shannon指数分别为1.7139、0.3927和0.5632,遗传距离范围为0.12~0.71。在遗传距离为0.180处将供试材料分为5个类群,聚类结果与区域高度一致。主成分分析表明,3个主成分的总变异量为45.04%,与聚类结果一致。利用SSR引物STL001、S192和S118构建了63份种质资源的DNA指纹图谱,并建立了二维码分子身份证。本研究为马铃薯资源利用和品种改良奠定基础。
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