水稻黄叶突变体ys94的鉴定与基因定位

doi:10.16035/j.issn.1001-7283.2016.04.007

摘要/Abstract

摘要：

水稻突变体ys94,是一个来源于化学诱变的叶色突变体。突变体在三叶期前出现明显的黄叶表型,叶绿素a、叶绿素b含量下降;透射电镜观察表明,突变体叶片叶绿体数量减少、类囊体片层退化。三叶期后叶色开始转绿。成熟期突变体ys94的分蘖数减少,但在结实率和株高等性状上与野生型相比并无明显差异。遗传分析表明,ys94的突变性状由一对隐性核基因控制。利用ys94×DJY组合的F₂定位群体,将突变基因定位在第8染色体短臂标记M8-3和M8-24之间,两标记间的物理距离为140kb,此区间包含11个候选基因。本研究结果为候选基因的克隆和功能分析奠定了基础。

关键词: 水稻, 叶色突变体, 精细定位, 黄叶, ys94

Abstract:

Abstrate A yellow-leaf mutant ys94 was isolated by chemical mutagenesis. Compared with its wild type, ys94 mutant displayed yellow leaf phenotype and reduced contents of both chlorophyll a and chlorophyll b in the germination period. The results of electron microscopic observation revealed that there were significantly reduced number of chloroplast,chloroplast dysplasia and degenerated thylakoid lamella in the ys94 in compared to WT.The tiller number of the mutant was reduced,but the plant height,the number of spikelets per panicle and the seed setting had no significant difference with the wild type. Genetic analysis showed that the mutation of ys94 was controlled by one recessive nuclear gene. Map-based mapping of the mutant gene was conducted by using of the F₂population from ys94×DJY. The mutant gene was finally mapped to a 140kb region between the maker M8-3 and M8-24 on the short arm of chromosome 8, in which there were 11 annotated genes. Our study results have set a foundation for future cloning and functional analysis of the gene.

Key words: Key words Rice, Leaf-colour mutant, Gene mapping, Yellow leaf, ys94

明鸣,汪颖,林启冰,王益华,程治军. 水稻黄叶突变体ys94的鉴定与基因定位[J]. 作物杂志, 2016 (4): 41–46

Ming Ming,Ying Wang,Qibing Lin,Yihua Wang,Zhijun Cheng. Identification and Gene Mapping of a Yellow-Leaf Rice Mutant ys94[J]. Crops, 2016(4): 41–46

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农艺性状Agronomic traits	野生型Wild type	突变体ys94	比对照增减Compared with WT(%)
株高Plant height(cm)	103.03±3.36	102.53±2.91	-0.49
分蘖数Tiller number(个)	9.89±1.69	8.91±1.42	-9.90^*
结实率Seed-setting rate(%)	82.93±1.71	84.41±1.30	1.70
有效分蘖数Effective tiller number(个)	9.26±1.60	8.66±1.36	-6.50^*
每穗粒数No. of spikelets per panicle(粒)	102.77±20.23	103.18±22.78	0.40
千粒重1000-kernel weight(g)	24.41±0.03	25.11±0.04	2.90