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作物杂志,2021, 第6期: 58–61 doi: 10.16035/j.issn.1001-7283.2021.06.009

• 遗传育种·种质资源·生物技术 • 上一篇    下一篇

利用高密度遗传图谱发掘水稻抽穗期新位点

苏代群1(), 陈亮1, 李锋1, 武琦1, 白君杰2, 邹德堂2, 王敬国2, 刘化龙2(), 郑洪亮2   

  1. 1黑龙江省种业技术服务中心,150008,黑龙江哈尔滨
    2东北农业大学寒地粮食作物种质创新与生理生态教育部重点实验室,150030,黑龙江哈尔滨
  • 收稿日期:2021-03-26 修回日期:2021-06-22 出版日期:2021-12-15 发布日期:2021-12-16
  • 通讯作者: 刘化龙
  • 作者简介:苏代群,主要从事水稻遗传育种研究,E-mail： sudaiqun@163.com
  • 基金资助:
    黑龙江省“百千万”工程生物育种重大科技专项“优质抗逆水稻新品种选育”(2020ZX16B01)

Identification of New Heading Date QTLs Using High Density Genetic Map in Rice

Su Daiqun1(), Chen Liang1, Li Feng1, Wu Qi1, Bai Junjie2, Zou Detang2, Wang Jingguo2, Liu Hualong2(), Zheng Hongliang2   

  1. 1Heilongjiang Province Seed Industry Technical Service Center, Harbin 150008, Heilongjiang, China
    2Key Laboratory of Germplasm Enhancement, Physiology and Ecology of Food Crops in Cold Region,Ministry of Education, Northeast Agricultural University, Harbin 150030, Heilongjiang, China
  • Received:2021-03-26 Revised:2021-06-22 Online:2021-12-15 Published:2021-12-16
  • Contact: Liu Hualong

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摘要：

水稻抽穗期是决定水稻种植地区及其季节适应性的关键因素,发掘控制水稻抽穗期相关的新主效QTL至关重要。利用包含527个bin标记的高密度遗传连锁图谱,通过靶向测序基因型检测技术对水稻“空育131/小白粳子”衍生的RIL群体进行抽穗期基因型分析。通过对双亲和RIL群体的基本统计分析发现,双亲抽穗期呈极显著差异,表型处于RIL群体范围内,RIL群体有明显的超亲分离现象,符合正态分布。利用IciMapping 4.2软件的完备区间作图法,在水稻第1、3和7号染色体上共检测到4个QTL,其中3个QTL区间内分别含有与抽穗期相关的已知基因OsGI、Hd6和Ghd7,而qHD-3-1是控制水稻抽穗期的新位点。

关键词: 水稻, 抽穗期, bin标记, QTL, 靶向测序基因型检测技术（GBTS）, 重组自交系

Abstract:

Heading date is the key factor to determine the rice planting area and its seasonal adaptability, so it is very important to find new major QTLs controlling rice heading date. The high-density genetic linkage map with 527 bin markers of the combinant inbred line (RIL) population derived from 'Kongyu 131/Xiaobaijingzi' was used for genotype analysis of heading date in rice by genotyping by target sequencing (GBTS). Through the basic statistical analysis of the parents and the RIL population, it was found that the parent's heading date was extremely significantly different, the phenotypes were within the range of the RIL population, and there was an obvious phenomenon of transgressive segregation that conformed to the normal distribution. Four QTLs were detected on chromosomes 1, 3, and 7 of rice using full interval mapping method of IciMapping 4.2. Among them, three QTLs contained known genes OsGI, Hd6 and Ghd7 related to heading stage, respectively. qHD-3-1 was a new QTL controlling the heading date of rice.

Key words: Rice, Heading date, Bin marker, QTL, Genotyping by target sequencing (GBTS), Recombinant inbred lines

表1

水稻抽穗期的基本统计分析

项目
Item		 亲本Parents 重组自交系群体RIL population
空育131
Kongyu 131 (d)		 小白粳子
Xiaobaijingzi (d)		 平均值
Mean (d)		 标准差
Standard deviation		 变异范围
Range (d)		 变异系数
Coefficient of variation (%)		 峰度
Kurtosis		 偏度
Skewness
抽穗期Heading date 91.00 104.00** 97.74 7.51 76.00~111.00 7.68 -0.42 -0.23

图1

水稻抽穗期QTL在染色体上的分布

表2

水稻抽穗期QTL及其遗传效应

数量性状位点
QTL		 染色体
Chromosome		 标记区间
Marker interval		 LOD值
LOD peak		 贡献率
Contribution rate (%)		 加性效应
Additive effect		 已知基因
Known gene
qHD-1-1 1 C1_4330799 C1_4645558 3.0181 4.0619 1.5969 OsGI
qHD-3-1 3 C3_799346 C3_1493308 7.0671 10.5098 2.7637
qHD-3-2 3 C3_29189855 C3_33130038 3.2297 4.5338 1.6866 Hd6
qHD-7-1 7 C7_8809828 C7_13201476 13.2288 21.7016 -3.8409 Ghd7
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