Crops ›› 2016, Vol. 32 ›› Issue (4): 93-100.doi: 10.16035/j.issn.1001-7283.2016.04.015

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Association Analysis of Fiber Quality with SSR Markers in Gossypium barbadense L.

Zhang Sujun1,Tang Liyuan1,Liu Cunjing1,Jiang Zhenxing2,Chi Jina1,Tian Haiyan1,Li Xinghe1,Zhang Jianhong1,Zhang Xiangyun1   

  1. 1 Key Laboratory Biology and Genetic Improvement of Cotton in Huanghuaihai Semiarid Area/National Cotton Improvement Center Hebei Branch,Ministry of Agriculture,P.R,China/Institute of Cotton,Hebei Academy of Agriculture and Forestry Sciences,Shijiazhuang 050051,Hebei,China
    2 Hebei Academy of Governance,Shijiazhuang 050031,Hebei,China
  • Received:2016-03-17 Revised:2016-06-06 Online:2016-08-15 Published:2018-08-26
  • Contact: Jianhong Zhang,Xiangyun Zhang

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Abstract:

In this study, 211 SSR loci, evenly distributed in 26 chromosomes, were assayed for polymorphism among 28 sea island cotton cultivars, a total of 627 alleles were identified at 241 SSR loci in sea island cotton cultivars amplified by 211 pairs of SSR primers, and the average variable sites was 2.60. The association analysis between SSR loci and 5 fiber quality traits (upper-half mean length, breaking tenacity, micronaire value, uniformity and elongation) were performed using GLM (general linear model) program in Tassel. the results showed: 64 SSR loci highly significantly associated with the traits were screened out from this population under GLM program (P<0.001),among which 21 SSR loci were notably correlated with phenotypic value in 3 environments simultaneously, and there were 9 loci associated with two or more traits simultaneously, These results would provide some referenced information for the utilization of sea island cotton.

Key words: Sea island cotton, Association analysis, SSR, Fiber quality

Table 1

Sea island cotton cultivars"

序号
Number		 材料名称
Name		 序号
Number		 材料名称
Name		 序号
Number		 材料名称
Name		 序号
Number		 材料名称
Name
1 新海3号 8 新海19号 15 新海27号 22 海选-1
2 新海13号 9 新海20号 16 新海28号 23 海选-2
3 新海14号 10 新海21号 17 新海32号 24 海选-3
4 新海15号 11 新海22号 18 新海36号 25 海7124
5 新海16号 12 新海23号 19 新海37号 26 新海1号
6 新海17号 13 新海25号 20 海陆1-7 27 Pima90-53
7 新海18号 14 新海26号 21 海陆1-9 28 新海2号

Table 2

211 SSR markers"

标记名称 Markers name
BNL1064 BNL2496A BNL3779 CGR6280 HAU0604 JESPR152 NAU2477 NAU3862 NAU6724
BNL1066 BNL2553 BNL3800 CGR6528 HAU1001 JESPR220 NAU2579 NAU3881 NAU6764
BNL1122 BNL256 BNL3806 CGR6680 HAU1219 JESPR291 NAU2611 NAU3911 NAU7140
BNL1151 BNL2572 BNL3811 CGR6756 HAU1384 JESPR296 NAU2639 NAU3946 NAU864
BNL1162 BNL2590 BNL3816 CGR6834 HAU1632 JESPR297 NAU2697 NAU3967 NAU933
BNL1227 BNL2597 BNL3888 CGR6894 HAU1741 JESPR304 NAU2713 NAU3991 NAU934
BNL1231 BNL2632 BNL3895 CIR246 HAU1749 JESPR50 NAU2715 NAU4030 NAU943
BNL1317 BNL2646 BNL3902 CIR286 HAU1754 JESPR6 NAU2723 NAU4057 STV030
BNL1440 BNL2650 BNL3955 CIR354 HAU1774 JESPR65 NAU2741 NAU4855 STV031
BNL1604 BNL2651 BNL3993 CIR362 HAU1780 MUCS101 NAU2771 NAU4921 STV177
BNL1606 BNL2652 BNL4053 CM056 HAU1802 MUCS135 NAU2826 NAU4962 NAU2734
BNL1646 BNL2656 BNL409 CM067 HAU1809 MUSS10 NAU2836 NAU5109
BNL1673 BNL2847 BNL686 CM13 HAU1811 MUSS162 NAU2859 NAU5260
BNL1681 BNL285 BNL827 COT002 HAU1830 MUSS181 NAU2887 NAU5307
BNL169 BNL3103 BNL834 CSHES HAU1842 MUSS193 NAU2902 NAU5323
BNL1694 BNL3259 CER0077 DPL0022 HAU1888 NAU1042 NAU2960 NAU5335
BNL1705 BNL3261 CGR5106 DPL0057 HAU1905 NAU1048 NAU3011 NAU5359
BNL1707 BNL3279 CGR5363 DPL0061 HAU2337 NAU1070 NAU3127 NAU5387
BNL1897 BNL3411 CGR5566 DPL0245 HAU2559 NAU1085 NAU3414 NAU5418
BNL193 BNL3442 CGR5688 DPL0282 HAU2738 NAU2002 NAU3519 NAU5428
BNL226 BNL3474 CGR5759 DPL0431 HAU3132 NAU2186 NAU3538 NAU5491
BNL243 BNL3590 CGR5803 DPL0492 HAU3241 NAU2190 NAU3607 NAU6173
BNL2440 BNL3594 CGR5880 Gh433 JESPR101 NAU2257 NAU3793 NAU6177
BNL2448 BNL3649 CGR5996 Gh441 JESPR110 NAU2265 NAU3816 NAU6431
BNL2449 BNL3650 CGR6019 Gh75 JESPR114 NAU2272 NAU3861 NAU6699

Table 3

Coefficient of variation of fiber quality in 7 environments"

纤维长度
Fiber length		 断裂比强度
Fiber strength		 马克隆值
Micronaire value		 伸长率
Elong rate		 整齐度
Uniformity
平均值
Mean		 变异系数
CV(%)		 平均值
Mean		 变异系数
CV(%)		 平均值
Mean		 变异系数
CV(%)		 平均值
Mean		 变异系数
CV(%)		 平均值
Mean		 变异系数
CV(%)
2013年石家庄Shijiazhuang 35.06 6.13 44.53 13.95 4.37 9.80 7.09 13.91 87.17 1.67
2014年石家庄Shijiazhuang 36.09 5.88 42.93 12.77 4.51 9.88 6.26 11.16 87.90 2.68
2014年威县Wei county 36.21 5.45 43.30 13.66 4.44 8.40 6.20 13.59 88.14 1.96
2014年海兴县Haixing county 34.39 8.49 39.62 15.89 3.98 20.58 6.56 15.76 85.70 2.88
2015年石家庄Shijiazhuang 35.87 7.80 42.28 14.16 4.33 8.78 8.20 12.42 88.23 2.27
2015年威县Wei county 35.61 6.63 41.21 11.42 4.29 9.47 7.93 11.59 88.63 1.98
2015年海兴县Haixing county 33.94 10.19 39.38 15.83 4.27 12.50 8.23 13.92 85.66 3.42

Fig.1

Dendrogram of 28 materials based on SSR"

Fig.2

Distribution of Kinship coefficients between 28 sea island cotton cultivars"

Fig.3

Distribution of linkage disequilibrium （LD） among SSR loci"

Table 4

SSR marker loci associated with fiber length and explanation of phenotypic variation %"

标记Marker 位置
Loci		 2013年
石家庄
Shijiazhuang		 2014年
石家庄
Shijiazhuang		 2014年
威县
Wei county		 2014年
海兴县
Haixing county		 2015年
石家庄
Shijiazhuang		 2015年
威县
Wei county		 2015年
海兴县
Haixing county
BNL1681 A11 32.48 41.20
CGR5996 A3 32.50 41.20
BNL3649 Non 44.68
BNL3650 A6 48.93

Table 5

SSR marker loci associated with fiber strength and explanation of phenotypic variation %"

标记
Marker		 位置
Loci		 2013年
石家庄
Shijiazhuang		 2014年
石家庄
Shijiazhuang		 2014年
威县
Wei county		 2014年
海兴县
Haixing county		 2015年
石家庄
Shijiazhuang		 2015年
威县
Wei county		 2015年
海兴县
Haixing county
BNL1897 A2 24.20 30.26 28.76
DPL0022 A5 41.36 47.81
CGR5880 A7 22.71
BNL2496A D3 32.73
BNL3474 D8 22.88
BNL3888 A1 22.75
NAU4057-1 A5 27.52
STV031 A10 37.74

Table 6

SSR marker loci associated with micronaire value and explanation of phenotypic variation %"

标记
Marker		 位置
Loci		 2013年石家庄
Shijiazhuang		 2014年石家庄
Shijiazhuang		 2014年威县
Wei county		 2014年海兴县
Haixing county		 2015年石家庄
Shijiazhuang		 2015年威县
Wei county		 2015年海兴县
Haixing county
BNL1604 A7 49.43 51.76 51.93
DPL0057 A12 53.15 45.69 36.12
NAU2826 D11 56.40 49.64 34.24
NAU5387 D13 51.09 45.00 36.17
NAU6431 D11 59.61 54.45 37.08
MUSS193 Non 54.77 61.23 40.75
CGR6894 D7 56.40 50.87 34.24
BNL3806 D6 57.00 52.87 34.88
NAU3862 A12 60.08 55.04 37.56
NAU4030-1 A7 49.60 45.67 29.11
NAU5260 D3 59.30 54.35 36.52
NAU5418 D11 49.60 45.67 29.11
BNL3806 Non 59.30 54.35 36.52
NAU934 A5 47.74 45.97
DPL0492 D7 44.25 30.91
NAU2579 D10 45.87 31.31
NAU3607 A5 32.12 50.97
NAU4030-2 A7 51.79 49.17
STV031 D10 62.36 64.24
CIR362 A12 51.89 60.37
JESPR50-1 A5 42.91 45.24
JESPR110 D9 38.33 48.57
标记
Marker		 位置
Loci		 2013年石家庄
Shijiazhuang		 2014年石家庄
Shijiazhuang		 2014年威县
Wei county		 2014年海兴县
Haixing county		 2015年石家庄
Shijiazhuang		 2015年威县
Wei county		 2015年海兴县
Haixing county
NAU864 D9 50.63 49.82
NAU1042-1 D5 47.83 51.85
NAU2186 A7 39.02 44.51
NAU2741 D5 58.54 55.74
NAU2771 D9 38.99 41.04
NAU3911 A1 38.99 41.04
NAU7140 D11 50.42 49.46
NAU3991 D13 58.02
JESPR114 Non 43.78
NAU2734 D7 30.88
NAU1042-2 D5 50.83
NAU2836 A3 45.41

Table 7

SSR marker loci associated with elong rate and explanation of phenotypic variation %"

标记
Marker		 位置
Loci		 2013年石家庄
Shijiazhuang		 2014年石家庄
Shijiazhuang		 2014年威县
Wei county		 2014年海兴县
Haixing county		 2015年石家庄
Shijiazhuang		 2015年威县
Wei county		 2015年海兴县
Haixing county
BNL1681 A11 56.71 46.74 46.51 50.45 60.19 58.10
STV030 D2 59.48 66.52 72.74 58.65 61.60
BNL3649 Non 56.83 42.72 49.53 48.71 53.73
JESPR110 D9 54.33 47.22 38.90
MUSS193 Non 58.01 61.08 62.77
BNL2590-2 A9 60.63 74.16 56.94
BNL1227-2 D12 72.30 83.87
BNL834 D3 65.93 79.23
BNL2572 A4 38.57 39.73
BNL1227-1 D12 76.35

Table 8

SSR marker loci associated with uniformity and explanation of phenotypic variation %"

标记
Marker		 位置
Loci		 2013年石家庄
Shijiazhuang		 2014年石家庄
Shijiazhuang		 2014年威县
Wei county		 2014年海兴县
Haixing county		 2015年石家庄
Shijiazhuang		 2015年威县
Wei county		 2015年海兴县
Haixing county
BNL2449 Non 52.66 47.21 39.24
BNL1681 A11 39.75 33.19
BNL3649 Non 40.51 33.46
CGR5996 A3 54.24
BNL3650 A6 42.44
DPL0022 A5 45.41
BNL2590-2 A9 41.26
MUSS193 Non 43.26
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