Crops ›› 2016, Vol. 32 ›› Issue (5): 13-18.doi: 10.16035/j.issn.1001-7283.2016.05.003

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SSR Analysis of Winter Wheat Lines of Beijing Regional Trials from 2009 to 2015

Liu Lihua1,Pang Binshuang1,Li Hongbo1,Liu Yangna1,Fu Deping2,Wang Na1,Zhao Changping1   

  1. 1 Beijing Engineering and Technique Research Center for Hybrid Wheat,Beijing Academy of Agriculture and Forestry Sciences/The Municipal Key Laboratory of the Molecular Genetics of Hybrid Wheat,Beijing 100097,China
    2 Beijing Seed Management Station,Beijing 100026,China
  • Received:2016-07-12 Revised:2016-08-22 Online:2016-10-15 Published:2018-08-26
  • Contact: Changping Zhao

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Abstract:

SSR Analysis of 130 winter wheat lines from Beijing regional trials from 2009 to 2015 could provide a theoretical basis for the wheat variety management and breeding. The SSR fingerprint detection results showed that thirty wheat lines had poor uniformity and stability, twenty-eight wheat lines were similar with regional trials lines or varieties, two wheat lines were different between two years. There were fewer problem wheat lines in 2013-2015. The genetic diversity showed that there were different degrees of genetic variations among all the regional trials lines, but the high frequency use of the backbone parents reduced the genetic diversity of the population. There was no significant change in genetic diversity among different years. In the future, in order to improve the genetic diversity and broaden the genetic background of winter wheat varieties in Beijing, the breeding resources, breeding patterns and breeding direction should be changed.

Key words: Beijing regional trials, Winter wheat, SSR fingerprint, Line

Table 1

Information of 21 pairs of primers"

引物
Primer		 染色体
Chromosome		 退火温度(℃)
Annealing temperature		 引物序列（5′-3′）
Primer sequence
cwm65
 1A
 65
 F：TCATTGGTGTCATCCCTCGTGT
R：GAATAATGCCTTGACCCTGGAC
barc80
 1BL
 65
 F：GCGAATTAGCATCTGCATCTGTTTGAG
R：CGGTCAACCAACTACTGCACAAC
cfd72
 1DL
 60
 F：CTCCTTGGAATCTCACCGAA
R：TCCTTGGGAATATGCCTCCT
gwm294
 2AL
 55
 F：GGATTGGAGTTAAGAGAGAACCG
R：GCAGAGTGATCAATGCCAGA
gwm429
 2BS
 55
 F：TTGTACATTAAGTTCCCATTA
R：TTTAAGGACCTACATGACAC
gwm261
 2DS
 55
 F：CTCCCTGTACGCCTAAGGC
R：CTCGCGCTACTAGCCATTG
gwm155
 3AL
 55
 F：CAATCATTTCCCCCTCCC
R：AATCATTGGAAATCCATATGCC
gwm285
 3BS
 65
 F：ATGACCCTTCTGCCAAACAC
R：ATCGACCGGGATCTAGCC
gdm72
 3DS
 55
 F：TGGTTTTCTCGAGCATTCAA
R：TGCAACGATGAAGACCAGAA
gwm610
 4AS
 65
 F：CTGCCTTCTCCATGGTTTGT
R：AATGGCCAAAGGTTATGAAGG
ksum62
 4B
 60
 F：GGAGAGGATAGGCACAGGAC
R：GAGAGCAGAGGGAGCTATGG
barc91
 4DL
 55
 F：TTCCCATAACGCCGATAGTA
R：GCGTTTAATATTAGCTTCAAGATCAT
cwem40
 5A
 58
 F：TAGCACCAGGCTTGACCAGT
R：GGACCAAAGCCAAAAACAAA
gwm67
 5BL
 60
 F：ACCACACAAACAAGGTAAGCG
R：CAACCCTCTTAATTTTGTTGGG
cfd29
 5DL
 65
 F：GGTTGTCAGGCAGGATATTTG
R：TATTGATAGATCAGGGCGCA
gwm334
 6AS
 55
 F：AATTTCAAAAAGGAGAGAGA
R：AACATGTGTTTTTAGCTATC
barc198
 6BS
 55
 F：CGCTGAAAAGAAGTGCCGCATTATGA
R：CGCTGCCTTTTCTGGATTGCTTGTCA
cfd76
 6DL
 65
 F：GCAATTTCACACGCGACTTA
R：CGCTCGACAACATGACACTT
cfa2028
 7AS
 55
 F：TGGGTATGAAAGGCTGAAGG
R：ATCGCGACTATTCAACGCTT
gwm333
 7BS
 60
 F：GCCCGGTCATGTAAAACG
R：TTTCAGTTTGCGTTAAGCTTTG
gwm437
 7DL
 55
 F：GATCAAGACTTTTGTATCTCTC
R：GATGTCCAACAGTTAGCTTA

Table 2

The number of the problematic wheat lines that were detected in Beijing"

年份
Year		 一致性和稳定性
Uniformity and stability		 疑似品系
Suspected line		 真实性
Genuineness
2009 3 8 0
2010 6 7 0
2011 10 2 1
2012 5 5 0
2013 1 1 0
2014 2 2 1
2015 3 3 0
合计Total 30 28 2

Fig.1

Genetic diversity comparison of wheat national regional trial in different years"

Fig.2

UPGMA cluster diagram of 114 wheat lines based on Nei’s genetic distance （1-114 were the codes of wheat lines）"

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