Crops ›› 2017, Vol. 33 ›› Issue (5): 73-79.doi: 10.16035/j.issn.1001-7283.2017.05.013

Previous Articles     Next Articles

Response of Antioxidant System to Drought Stress in Sugar Beet Leaves at Seedling Stage

Li Guolong1,Sun Yaqing1,Shao Shiqin2,Zhang Yongfeng1   

  1. 1Agricultural College,Inner Mongolia Agricultural University,Hohhot 010018,Inner Mongolia,China
    2College of Life Sciences,Inner Mongolia Agricultural University,Hohhot 010018,Inner Mongolia,China
  • Received:2017-07-22 Revised:2017-08-16 Online:2017-10-15 Published:2018-08-26

RichHTML

11

PDF (PC)

139

Abstract:

Two sugar beet cultivars HI0466 and KWS9454, different in drought resistance, were used to examine the response of antioxidant system to drought stress and the relationship for evaluating drought resistance during seedling stage under different drought conditions. The results showed that activities of protective enzymes SOD, POD, CAT and contents of GSH, AsA were increased significantly and then decreased significantly, however, carotenoids (Car) content decreased under drought stress. To resistant cultivar, the increase was more significant than the decrease, so the activities of SOD, POD and CAT and the contents of Car, AsA were higher, meanwhile, malondialdehyde (MDA) contents were lower under several treatments. There was significant difference between HI0466 and KWS9454. In addition, it was found that the activities of SOD, POD and contents of MDA, AsA could be used as effective indicators for the identification of drought resistance at seedling stage for sugar beet species by principal component analysis.

Key words: Sugar beet, Antioxidant system, Drought stress, Drought resistance

Fig.1

The change of SOD activity of sugar beet seedling leaves under different water conditions RW1, RW2 indicate re-water treatment of KWS9454, HI0466, respectively, the same below"

Fig.2

The change of POD activity of sugar beet seedling leaves under different water conditions"

Fig.3

The change of CAT activity of sugar beet seedling leaves under different water conditions"

Fig.4

The change of MDA content of sugar beet seedling leaves under different water conditions"

Fig.5

The change of H2O2 content of sugar beet seedling leaves under different water conditions"

Fig.6

The change of Car content of sugar beet seedling leaves under different water conditions"

Fig.7

The change of GSH content of sugar beet seedling leaves under different water conditions"

Fig.8

The change of AsA content of sugar beet seedling leaves under different water conditions"

Table 1

Eigenvectors and percentages of accumulated contribution of principal components"

生理指标
Physiological index		 成分1
Component 1		 成分2
Component 2
SOD活性SOD activity 0.977 0.425
POD活性POD activity 0.941 0.751
MDA含量MDA content 0.388 -0.972
CAT活性CAT activity 0.847 0.591
H2O2含量H2O2 content 0.718 -0.139
Car含量Car content -0.555 0.849
GSH含量GSH content 0.455 -0.089
AsA含量AsA content -0.232 0.917
特征值Eigen value 3.786 3.444
贡献率Contribution rate (%) 47.325 43.046
累计百分率Cumulative percentage (%) 47.325 90.372
[1] 黄玉兰, 殷奎德, 向君亮 , 等. 干旱胁迫下植物生理生化及DNA甲基化的研究进展. 玉米科学, 2016,24(2):96-102.
[2] Schindler U, Steidl J, Müller L , et al. Drought risk to agricultural land in Northeast and Central Germany. Journal of Plant Nutrition & Soil Science, 2007,170(3):357-362.
doi: 10.1002/jpln.200622045
[3] 山仑 . 植物抗旱生理研究与发展半旱地农业. 干旱地区农业研究, 2007,25(1):1-5.
doi: 10.3321/j.issn:1000-7601.2007.01.001
[4] Nogués I, Llusià J, Ogaya R , et al. Physiological and antioxidant responses of Quercus ilex to drought in two different seasons. Plant Biosystems, 2014,148(2):268-278.
doi: 10.1080/11263504.2013.768557
[5] 邵惠芳, 陈征, 许嘉阳 , 等. 两种烟草幼苗叶片对不同强度干旱胁迫的生理响应比较.植物生理学报, 2016(12):1861-1871.
[6] Ma X, Xin Z, Wang Z , et al. Identification and comparative analysis of differentially expressed miRNAs in leaves of two wheat (Triticum aestivum L.) genotypes during dehydration stress. BMC Plant Biology, 2015,15(1):1-15.
doi: 10.1186/s12870-014-0410-4 pmid: 4311480
[7] Chaves M M, Maroco J P, Pereira J S . Understanding plant responses to drought-from genes to the whole plant. Functional Plant Biology, 2003,30(3):239-264.
doi: 10.1071/FP02076
[8] 靳军英, 张卫华, 袁玲 . 三种牧草对干旱胁迫的生理响应及抗旱性评价. 草业学报, 2015,24(10):157-165.
doi: 10.11686/cyxb2014505
[9] 任磊, 赵夏陆, 许靖 , 等. 4种茶菊对干旱胁迫的形态和生理响应. 生态学报, 2015,35(15):5131-5139.
doi: 10.5846/stxb201401220164
[10] 郭启芳, 吴耀领, 王玮 . 干旱、高温及共胁迫下不同小麦品种的生理生化响应差异.山东农业科学, 2014(11):32-38.
doi: 10.3969/j.issn.1001-4942.2014.11.009
[11] 张旭东, 王智威, 韩清芳 , 等. 玉米早期根系构型及其生理特性对土壤水分的响应. 生态学报, 2016,36(10):2969-2977.
doi: 10.5846/stxb201409181852
[12] 马一泓, 王术, 于佳禾 , 等. 水稻生长对干旱胁迫的响应及抗旱性研究进展. 种子, 2016,35(7):45-49.
doi: 10.16590/j.cnki.1001-4705.2016.07.045
[13] 张木清, 陈如凯 . 作物抗旱分子生理与遗传改良.北京: 科学出版社, 2005: 22-23.
[14] 李丽芳, 罗晓芳, 王华芳 . 植物抗旱基因工程研究进展. 西北林学院学报, 2004,19(3):53-57.
[15] 李国龙, 吴海霞, 温丽 , 等. 甜菜苗期抗旱鉴定指标筛选及其综合评价. 干旱地区农业研究, 2011,29(4):69-74.
[16] 高俊凤 . 植物生理学实验指导.北京: 高等教育出版社, 2006: 221-224.
[17] 张志良, 瞿伟菁 . 植物生理学实验指导.3版.北京: 高等教育出版社, 2006: 274-277.
[18] 王学奎 . 植物生理生化实验原理和技术.2版.北京: 高等教育出版社, 2006: 172-173.
[19] 张宗申, 利容千, 王建波 . 外源Ca 2+预处理对高温胁迫下辣椒叶片细胞膜透性和GSH、AsA含量及Ca 2+分布的影响 . 植物生态学报, 2001,25(2):230-234.
[20] 高俊凤 . 植物生理学试验技术.西安: 世界图书出版公司, 2000: 101-103.
[21] 夏民旋, 王维, 袁瑞 , 等. 超氧化物歧化酶与植物抗逆性. 分子植物育种, 2015,13(11):2633-2646.
doi: 10.3969/j.issn.1002-2767.2002.01.012
[22] 左应梅, 杨维泽, 杨天梅 , 等. 干旱胁迫下4种人参属植物抗性生理指标的比较.作物杂志, 2016(3):84-88.
doi: 10.16035/j.issn.1001-7283.2016.03.016
[23] 闫志利, 牛俊义, 席玲玲 , 等. 水分条件对豌豆保护酶活性及膜脂过氧化的影响. 中国生态农业学报, 2009,17(3):554-559.
doi: 10.3724/SP.J.1011.2009.00554
[24] 杜彩艳, 段宗颜, 潘艳华 , 等. 干旱胁迫对玉米苗期植株生长和保护酶活性的影响. 干旱地区农业研究, 2015,33(3):124-129.
[25] 井大炜, 邢尚军, 杜振宇 , 等. 干旱胁迫对杨树幼苗生长、光合特性及活性氧代谢的影响. 应用生态学报, 2013,24(7):1809-1816.
[26] 孙彩霞, 刘志刚, 荆艳东 . 水分胁迫对玉米叶片关键防御酶系活性及其同工酶的影响. 玉米科学, 2003,11(1):63-66.
doi: 10.3969/j.issn.1005-0906.2003.01.021
[27] Kolarovič L, Valentovič P, Luxová M , et al. Changes in antioxidants and cell damage in heterotrophic maize seedlings differing in drought sensitivity after exposure to short-term osmotic stress. Plant Growth Regulation, 2009,59(1):21-26.
doi: 10.1007/s10725-009-9384-x
[28] 马俊莹, 周睿, 程炳嵩 . 类胡萝卜素与活性氧代谢的关系.山东农业大学学报(自然科学版), 1997(4):518-521.
[29] Fletcher J M, Barnes J D, Foyer C H . The function of ascorbate oxidase in tobacco. Plant Physiology, 2003,132(3):1631-1641.
doi: 10.1104/pp.103.022798 pmid: 12857842
[30] 阎成仕, 李德全, 张建华 . 冬小麦旗叶旱促衰老过程中氧化伤害与抗氧化系统的响应. 西北植物学报, 2000,20(4):568-576.
doi: 10.3321/j.issn:1000-4025.2000.04.013
[31] 赵丽英, 邓西平, 山仑 . 活性氧清除系统对干旱胁迫的响应机制. 西北植物学报, 2005,25(2):413-418.
[32] 张盼盼, 冯佰利, 王鹏科 , 等. PEG胁迫下糜子苗期抗旱指标鉴选研究. 中国农业大学学报, 2012,17(1):53-59.
[33] Srivastava S, Srivastava M . Morphological changes and antioxidant activity of Stevia rebaudiana under water stress. American Journal of Plant Sciences, 2014,5(22):3417-3422.
doi: 10.4236/ajps.2014.522357
[34] 张智猛, 万书波, 戴良香 , 等. 花生抗旱性鉴定指标的筛选与评价. 植物生态学报, 2011,35(1):100-109.
doi: 10.3724/SP.J.1258.2011.00100
[35] 郭数进, 李玮瑜, 马艳芸 , 等. 山西不同生态型大豆品种苗期耐低温性综合评价. 植物生态学报, 2014,38(9):990-1000.
doi: 10.3724/SP.J.1258.2014.00093
[36] 田山君, 杨世民, 孔凡磊 , 等. 西南地区玉米苗期抗旱品种筛选. 草业学报, 2014,23(1):50-57.
doi: 10.11686/cyxb20140107
[1] Jiao Zhang,Qi Wu,Yufei Zhou,Yitao Wang,Ruidong Zhang,Ruidong Huang. Effects of Drought and Rewatering at Seedling and Filling Stages on Photosynthetic Characteristics and Dry Matter Production of Sorghum [J]. Crops, 2018, 34(3): 148-154.
[2] Shuguang Wang,Yugang Shi,Huawei Shi,Yaping Cao,Daizhen Sun. Research on Relationship between Photosynthetic Characteristics and Drought Resistance in Spring Wheat [J]. Crops, 2017, 33(6): 23-29.
[3] Wenwen Ji,Zeyan Zhang,Yaowen Zhang,Daizhen Sun. Identification of Drought Resistance of Adzuki Bean Germplasm Resources from Different Places in Budding Stage [J]. Crops, 2017, 33(3): 54-59.
[4] Junying Wu,Li Qin,Jin Yang,Dezhi Qin. Study of Salt Stress on Sugar Beet Seedling Growth and Nutrient Transport of Nongda Tianyan No. 6 [J]. Crops, 2017, 33(3): 75-80.
[5] Chang Xu,Jinghui Liu,Yanming Yang,Xue Bai,Bin Ma,Xinglong Zhang,Mengyuan Sun,Mengyu Zhang. Effects of Bacterial Manure and Nitrogen and Phosphorus Fertilizer Combination on Drought Resistance Physiological Indexes and Yield of Potato under Plastic-Mulching [J]. Crops, 2017, 33(1): 94-99.
[6] Jiao Wang,Yongqing Zhang. Observation on Growth Pattern of Root System in Different Adzuki Bean Varieties under Drought Stress [J]. Crops, 2016, 32(6): 112-119.
[7] Tongxin An,Mengli Chen,Feng Zhou,Jing Lu,Bozhi Wu,Lian'gen Zhang. Yield Benefit of Maize Planting Mode with Ditching and Pitting and Polythene Mulch Intercropping with Potato [J]. Crops, 2016, 32(5): 106-111.
[8] Limin Sang,Caifeng Li,Yubo Wang,Lei Liu,Guanghao Guo,Jian Guo,Ming Chen. Effects of Na2SO4+NaCl Mixed Salt Stress on the Growth of Sugar Beet Seedlings [J]. Crops, 2016, 32(4): 137-141.
[9] Yongxia Ren,Yupin Guo,Guihe Liu,Fang Sun,Chunmei Cao,Caiyue Xiao. Drought Resistance of Three Forage Species in Vicia during Germination Period [J]. Crops, 2016, 32(3): 158-162.
[10] Yingmei Zuo,Weize Yang,Tianmei Yang,Meiquan Yang,Zongliang Xu,Shaobing Yang,Jinyu Zhang. Comparison of Resistant Physiological Index among Four Species in the Genus Panax under Water Stress [J]. Crops, 2016, 32(3): 84-88.
[11] Huatao Liu,Xuefang Huang,Mingjing Huang,Baoliang Chi,Xiuqing Zheng,Junfeng Chen. Effects of Drought Stress at Jointing Stage on Yield and Drought Resistance in Spring Maize [J]. Crops, 2016, 32(2): 89-94.
[12] Xiaodong Dai,Xinzhi Xu,Cancan Zhu,Yufeng Yang,Chunyi Wang,Xiaoping Yang,Guohong Yang,Junxia Li. Seeding Stage Response to Different Water Availability and Drought Resistance Evaluation of Foxtail Millet [J]. Crops, 2016, 32(1): 140-143.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] Guangcai Zhao,Xuhong Chang,Demei Wang,Zhiqiang Tao,Yanjie Wang,Yushuang Yang,Yingjie Zhu. General Situation and Development of Wheat Production[J]. Crops, 2018, 34(4): 1 -7 .
[2] Baoquan Quan,Dongmei Bai,Yuexia Tian,Yunyun Xue. Effects of Different Leaf-Peg Ratio on Photosynthesis and Yield of Peanut[J]. Crops, 2018, 34(4): 102 -105 .
[3] Xuefang Huang,Mingjing Huang,Huatao Liu,Cong Zhao,Juanling Wang. Effects of Annual Precipitation and Population Density on Tiller-Earing and Yield of Zhangzagu 5 under Film Mulching and Hole Sowing[J]. Crops, 2018, 34(4): 106 -113 .
[4] Wenhui Huang, Hui Wang, Desheng Mei. Research Progress on Lodging Resistance of Crops[J]. Crops, 2018, 34(4): 13 -19 .
[5] Yun Zhao,Cailong Xu,Xu Yang,Suzhen Li,Jing Zhou,Jicun Li,Tianfu Han,Cunxiang Wu. Effects of Sowing Methods on Seedling Stand and Production Profit of Summer Soybean under Wheat-Soybean System[J]. Crops, 2018, 34(4): 114 -120 .
[6] Mei Lu,Min Sun,Aixia Ren,Miaomiao Lei,Lingzhu Xue,Zhiqiang Gao. Effects of Spraying Foliar Fertilizers on Dryland Wheat Growth and the Correlation with Yield Formation[J]. Crops, 2018, 34(4): 121 -125 .
[7] Xiaofei Wang,Haijun Xu,Mengqiao Guo,Yu Xiao,Xinyu Cheng,Shuxia Liu,Xiangjun Guan,Yaokun Wu,Weihua Zhao,Guojiang Wei. Effects of Sowing Date, Density and Fertilizer Utilization Rate on the Yield of Oilseed Perilla frutescens in Cold Area[J]. Crops, 2018, 34(4): 126 -130 .
[8] Pengjin Zhu,Xinhua Pang,Chun Liang,Qinliang Tan,Lin Yan,Quanguang Zhou,Kewei Ou. Effects of Cold Stress on Reactive Oxygen Metabolism and Antioxidant Enzyme Activities of Sugarcane Seedlings[J]. Crops, 2018, 34(4): 131 -137 .
[9] Jie Gao,Qingfeng Li,Qiu Peng,Xiaoyan Jiao,Jinsong Wang. Effects of Different Nutrient Combinations on Plant Production and Nitrogen, Phosphorus and Potassium Utilization Characteristics in Waxy Sorghum[J]. Crops, 2018, 34(4): 138 -142 .
[10] Na Shang,Zhongxu Yang,Qiuzhi Li,Huihui Yin,Shihong Wang,Haitao Li,Tong Li,Han Zhang. Response of Cotton with Vegetative Branches to Plant Density in the Western of Shandong Province[J]. Crops, 2018, 34(4): 143 -148 .