Crops ›› 2016, Vol. 32 ›› Issue (5): 19-24.doi: 10.16035/j.issn.1001-7283.2016.05.004

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Optimization of the Total Flavonoids Extraction Technology in Sugarcane Leaves

Shi Jingyu,He Lilian,Wang Xianhong,Li Fusheng   

  1. College of Agronomy and Biotechnology,Yunnan Agricultural University,Kunming 650201,Yunnan,China
  • Received:2016-07-12 Revised:2016-09-01 Online:2016-10-15 Published:2018-08-26
  • Contact: Fusheng Li

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Abstract:

To determine the optimal extraction conditions of flavonoids in sugarcane leaves by spectrometric method, sugarcane variety Dianzhe 01-58 selected by Yunan Agricultural University was used as material and rutin as standard. The different types of flavonoids of leaves both in wild species and cultivated varieties of sugarcane were determined by the index of extraction rate based on single factor and orthogonal test. The results showed that 70% (V/V), 70℃ and 90 minutes were the optimal ethanol concentration, extracting temperature and extracting duration, respectively. The flavonoids production were significantly different among different sugarcane types, Dianzhe 09-38 showed the highest flavonoids production in cultivated sugarcane and Miscanthus floridulus clone Ι 91-27 also showed the highest flavonoids production in wild sugarcane species. The results suggested that the flavonoids content in wild species were higher than that of cultivated species.

Key words: Sugarcane, Leaf, Total flavonoids, Extraction rate

Table 1

The sugarcane materials used for test"

栽培品种（系）
Cultivated variety (Line)		 野生种Wild species
桂糖11 Guitang 11 割手密88-269 Geshoumi 88-269
新台糖10 Xintaitang 10 割手密91-56 Geshoumi 91-56
新台糖22 Xintaitang 22 割手密93-192 Geshoumi 93-192
滇蔗01-58 Dianzhe 01-58 斑茅Ⅰ91-39 Banmao Ⅰ91-39
滇蔗01-106 Dianzhe 01-106 斑茅Ⅱ91-125 BanmaoⅡ91-125
滇蔗02-7 Dianzhe 02-7 斑茅93-59 Banmao 93-59
滇蔗04-14 Dianzhe 04-14 蔗茅90-43 Zhemao 90-43
滇蔗09-38 Dianzhe 09-38
 蔗茅IND81-001 (引自美国)
Zhemao IND81-001(from America)
滇蔗11-138 Dianzhe 11-138 五节芒Ι91-27 Wujiemang Ι91-27
滇蔗11-485 Dianzhe 11-485 五节芒Ⅱ91-52 Wujiemang Ⅱ91-52

Fig.1

Standard curve of rutin"

Fig.2

Effects of ethanol concentration on total flavonoids yield of sugarcane leaves"

Fig.3

Effects of extraction temperature on total flavonoids yield of sugarcane leaves"

Fig.4

Effects of extraction time on total flavonoids yield of sugarcane leaves"

Table 2

Factors and levels of the orthogonal experiment"

水平Level A乙醇浓度Ethanol concentration(%) B提取温度Extraction temperature(℃) C提取时间Extraction duration(min)
1 70 50 30
2 80 60 60
3 90 70 90

Table 3

The design and results of total flavonoids extraction using orthogonal experiment"

水平
Level		 A乙醇浓度(%)
Ethanol concentration		 B提取温度(℃)
Extraction temperature		 C提取时间(min)
Extraction duration		 提取率(%)
Total flavonoids yield
1 1 1 1 1.084
2 1 2 2 1.369
3 1 3 3 1.667
4 2 1 2 1.011
5 2 2 3 1.380
6 2 3 1 1.472
7 3 1 3 1.105
8 3 2 1 1.207
9 3 3 2 1.438
K1 4.120 3.856 2.679 11.733
K2 3.863 3.956 3.818
K3 3.750 4.577 4.152
k1 1.373 1.285 0.893
k2 1.288 1.319 1.273
k3 1.250 1.526 1.384
极差R 0.123 0.241 0.491

Table 4

The total flavonoids yield on different sugarcane varieties"

栽培品种（系）
Cultivated variety(Line)		 提取率(%)
Total flavonoids yield		 野生种
Wild species		 提取率(%)
Total flavonoids yield
滇蔗01-58 Dianzhe 01-58 1.718 斑茅93-59 Banmao 93-59 3.494
滇蔗01-106 Dianzhe 01-106 1.197 斑茅191-39 Banmao 191-39 2.830
滇蔗02-7 Dianzhe 02-7 1.575 斑茅Ⅱ91-125 BanmaoⅡ91-125 2.042
滇蔗04-14 Dianzhe 04-14 1.543 割手密88-269 Geshoumi 88-269 3.730
滇蔗09-38 Dianzhe 09-38 2.032 割手密Ⅰ91-56 GeshoumiⅠ 91-56 3.474
滇蔗11-138 Dianzhe 11-138 1.444 割手密93-192 Geshoumi 93-192 2.521
滇蔗11-485 Dianzhe 11-485 1.006 蔗茅90-43 Zhemao 90-43 3.225
新台糖10 Xintaitang 10 1.547 美蔗茅IND81-001 Meizhemao IND81-001 3.341
新台糖22 Xintaitang 22 1.420 五节芒Ⅰ91-27 Wujiemang Ⅰ91-27 5.840
桂糖11 Guitang 11 1.609 五节芒Ⅱ91-52 3.313
平均值Mean 1.509 平均值 Mean 3.381
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