Crops ›› 2019, Vol. 35 ›› Issue (6): 162-167.doi: 10.16035/j.issn.1001-7283.2019.06.026

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Identification of Bacterium HT-6 and Its Antagonistic Stability against Phytophthora infestans

Zhang Congying,Jiang Jizhi,Liang Jiao,Qiao Liu,Huang Jie   

  1. College of Life Sciences, Hebei University, Baoding 071002, Hebei, China
  • Received:2019-04-28 Revised:2019-06-12 Online:2019-12-15 Published:2019-12-11
  • Contact: Jizhi Jiang

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Abstract:

In order to clarify the biological characteristics and antagonistic stability of bacterium HT-6 strain on Phytophthora infestans, this experiment was conducted to observe its colony features on different media, detect physiological and biochemical characteristics and analyze 16S rDNA sequence, to determine its classification status. The effects of different temperature, pH, ultraviolet light, and continuous passage on the mycelial growth of Phytophthora infestans were tested. The HT-6 strain was identified as Bacillus subtilis based on results of its colony properties, physiological and biochemical characteristics and 16S rDNA sequence. The inhibition rates of HT-6 strain and its metabolites on P. infestans could keep more than 80% after treatment with 100℃ for 60min, pH 6-8 for 24h, 30W ultraviolet irradiation for 8h, and continuous 10 passages. These results showed that HT-6 strain and its metabolites had thermostability, ultraviolet resistance, and could store for a long time, except toleration acid and alkali. It has great potential value to develop it into a biological control agent for the prevention of potato late blight.

Key words: Potato, Strain identification, 16S rDNA, Bacteriostatic stability, Bacillus subtilis

Fig.1

Phylogenetic tree of HT-6 based on 16S rDNA sequence"

Table 1

Comparison of partial physiological and biochemical characteristics between HT-6 and its similar species"

试验Test HT-6 BS224[16] LXY-6[17] JY-1[18]
淀粉水解试验
Starch hydrolysis test		 + + + +
L-阿拉伯糖利用试验
L-arabionse utilization test		 + + + +
葡萄糖利用试验
Glucose utilization test		 + + + +
乳糖利用试验
Lactose utilization test		 + + ND -
木糖利用试验
Xylose utilization test		 + + + +
V. P试验V. P test + + - +
接触酶试验
Contact enzyme test		 + + + +
H2S试验H2S test + + + ND
溶血试验Hemolysis test + + + +
5% NaCl耐受试验
5% NaCl tolerance test		 + + + +
14% NaCl耐受试验
14% NaCl tolerance test		 - - + -
30% NaCl耐受试验
30% NaCl tolerance test		 - - + -
蛋白水解试验
Proteolysis test		 + + ND ND
色素试验Pigment test - - ND ND

Fig.2

Test of bacteriostatic stability of HT-6 strain bacterial solution Note: Different lowercase letters are significant difference at 0.05 level"

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