Crops ›› 2024, Vol. 40 ›› Issue (1): 180-186.doi: 10.16035/j.issn.1001-7283.2024.01.024

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Effects of Uniconazole Combined with Melatonin on Germination and Root Morphology of Adzuki Bean

Liu Jia1,2(), Wu Tianyi1, Zhu Jiayu1, Deng Shaozhu1, Zhang Yuxian1,2, Liang Xilong1, Jin Xijun1,2()   

  1. 1College of Agriculture, Heilongjiang Bayi Agricultural University, Daqing 163319, Heilongjiang, China
    2National Coarse Cereals Engineering Technology Research Center, Daqing 163319, Heilongjiang, China
  • Received:2022-11-21 Revised:2023-09-25 Online:2024-02-15 Published:2024-02-20
  • Contact: Jin Xijun E-mail:l197209elieensbing@163.com;shaoxiang1979@163.com

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Abstract:

In order to improve the quality of seedlings by treating seeds with plant growth regulators, this study set the concentration of uniconazole at 2, 4, 8, 16 and 32 mg/L, and the above concentration of uniconazole and 100 μmol/L melatonin compound coating treatment, with no drug solution coating as the control, to study the effects of different concentrations of uniconazole and its combination with melatonin on seed germination, root morphological characteristics, antioxidant enzyme activities and sugar contents of adzuki bean. The results showed that the germination index of adzuki bean treated with 8 mg/L uniconazole (S8) and its combination with melatonin (S8M) were significantly higher than that of the control, and the germination rate was not significantly different compared with control. With the increase of uniconazole concentration, the contents of fructose, soluble sugar and soluble protein in roots showed a trend of first up and then down, all of which were the highest in S8 treatment, while the contents of sucrose and starch in roots did not show obvious regularity. Compared with other treatments, S8 and S8M treatments were more conducive to maintaining a high level of antioxidant enzyme activities and significantly improving root activity. Correlation analysis showed that there were significant and extremely significant correlations between the above physiological indexes and root morphological indexes. Comprehensive analysis showed that 8 mg/L uniconazole coating and its combination with 100 μmol/L melatonin coating could promote the germination and root development of adzuki bean, which was conducive to the emergence of neat and strong seedlings.

Key words: Adzuki bean, Uniconazole, Melatonin, Germination, Root morphogenesis

Table 1

Coating composition"

处理Treatment 药剂及浓度Reagent and concentration
CK S3307 0 mg/L+MT 0 μmol/L
S2 S3307 2 mg/L
S2M S3307 2 mg/L+MT 100 μmol/L
S4 S3307 4 mg/L
S4M S3307 4 mg/L+MT 100 μmol/L
S8 S3307 8 mg/L
S8M S3307 8 mg/L+MT 100 μmol/L
S16 S3307 16 mg/L
S16M S3307 16 mg/L+MT 100 μmol/L
S32 S3307 32 mg/L
S32M S3307 32 mg/L+MT 100 μmol/L

Fig.1

Effects of various concentrations of uniconazole combined with melatonin on the germination of adzuki bean The top and bottom of the box represent the upper quartile (Q3) and lower quartile (Q1) of the data respectively, and the horizontal line in the box represents the median (Q2) of the data; The upper and lower edges represent the maximum and minimum values of this group of data, respectively; Points represent abnormal values; Different letters show significant difference among different treatments (P < 0.05). The same below."

Fig.2

Effects of various concentrations of uniconazole combined with melatonin on the sugar and soluble protein contents in adzuki bean roots"

Fig.3

Effects of various concentrations of uniconazole combined with melatonin on the antioxidative enzyme activities of adzuki bean roots"

Fig.4

Effects of various concentrations of uniconazole combined with melatonin on the root morphology and activity of adzuki bean"

Fig.5

Correlation analysis of physiological indexes with root morphology and activity SU: sucrose content; FR: fructose content; SS: soluble sugar content; ST: starch content; SP: soluble protein content; RL: root length; RSA: root surface area; RD: root diameter; RV: root volume; NLR: number of lateral roots; RA: root activity.“*”indicates significant correlation (P < 0.05),“**”indicates extremely significant correlation (P < 0.01),“***”indicates extremely significant correlation (P < 0.001)."

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