Crops ›› 2024, Vol. 40 ›› Issue (1): 180-186.doi: 10.16035/j.issn.1001-7283.2024.01.024

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Effects of Uniconazole Combined with Melatonin on Germination and Root Morphology of Adzuki Bean

Liu Jia1,2(), Wu Tianyi1, Zhu Jiayu1, Deng Shaozhu1, Zhang Yuxian1,2, Liang Xilong1, Jin Xijun1,2()   

  1. 1College of Agriculture, Heilongjiang Bayi Agricultural University, Daqing 163319, Heilongjiang, China
    2National Coarse Cereals Engineering Technology Research Center, Daqing 163319, Heilongjiang, China
  • Received:2022-11-21 Revised:2023-09-25 Online:2024-02-15 Published:2024-02-20
  • Contact: Jin Xijun E-mail:l197209elieensbing@163.com;shaoxiang1979@163.com

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Abstract:

In order to improve the quality of seedlings by treating seeds with plant growth regulators, this study set the concentration of uniconazole at 2, 4, 8, 16 and 32 mg/L, and the above concentration of uniconazole and 100 μmol/L melatonin compound coating treatment, with no drug solution coating as the control, to study the effects of different concentrations of uniconazole and its combination with melatonin on seed germination, root morphological characteristics, antioxidant enzyme activities and sugar contents of adzuki bean. The results showed that the germination index of adzuki bean treated with 8 mg/L uniconazole (S8) and its combination with melatonin (S8M) were significantly higher than that of the control, and the germination rate was not significantly different compared with control. With the increase of uniconazole concentration, the contents of fructose, soluble sugar and soluble protein in roots showed a trend of first up and then down, all of which were the highest in S8 treatment, while the contents of sucrose and starch in roots did not show obvious regularity. Compared with other treatments, S8 and S8M treatments were more conducive to maintaining a high level of antioxidant enzyme activities and significantly improving root activity. Correlation analysis showed that there were significant and extremely significant correlations between the above physiological indexes and root morphological indexes. Comprehensive analysis showed that 8 mg/L uniconazole coating and its combination with 100 μmol/L melatonin coating could promote the germination and root development of adzuki bean, which was conducive to the emergence of neat and strong seedlings.

Key words: Adzuki bean, Uniconazole, Melatonin, Germination, Root morphogenesis

Table 1

Coating composition"

处理Treatment 药剂及浓度Reagent and concentration
CK S3307 0 mg/L+MT 0 μmol/L
S2 S3307 2 mg/L
S2M S3307 2 mg/L+MT 100 μmol/L
S4 S3307 4 mg/L
S4M S3307 4 mg/L+MT 100 μmol/L
S8 S3307 8 mg/L
S8M S3307 8 mg/L+MT 100 μmol/L
S16 S3307 16 mg/L
S16M S3307 16 mg/L+MT 100 μmol/L
S32 S3307 32 mg/L
S32M S3307 32 mg/L+MT 100 μmol/L

Fig.1

Effects of various concentrations of uniconazole combined with melatonin on the germination of adzuki bean The top and bottom of the box represent the upper quartile (Q3) and lower quartile (Q1) of the data respectively, and the horizontal line in the box represents the median (Q2) of the data; The upper and lower edges represent the maximum and minimum values of this group of data, respectively; Points represent abnormal values; Different letters show significant difference among different treatments (P < 0.05). The same below."

Fig.2

Effects of various concentrations of uniconazole combined with melatonin on the sugar and soluble protein contents in adzuki bean roots"

Fig.3

Effects of various concentrations of uniconazole combined with melatonin on the antioxidative enzyme activities of adzuki bean roots"

Fig.4

Effects of various concentrations of uniconazole combined with melatonin on the root morphology and activity of adzuki bean"

Fig.5

Correlation analysis of physiological indexes with root morphology and activity SU: sucrose content; FR: fructose content; SS: soluble sugar content; ST: starch content; SP: soluble protein content; RL: root length; RSA: root surface area; RD: root diameter; RV: root volume; NLR: number of lateral roots; RA: root activity.“*”indicates significant correlation (P < 0.05),“**”indicates extremely significant correlation (P < 0.01),“***”indicates extremely significant correlation (P < 0.001)."

[1] Guilfoyle T, Hagen G, Gazzarrini S, et al. Hormone cross-talk during seed germination. Essays in Biochemistry, 2015, 58:151-164.
doi: 10.1042/bse0580151 pmid: 26374893
[2] Pacifici E, Polverari L, Sabatini S. Plant hormone cross-talk: the pivot of root growth. Journal of Experimental Botany, 2015, 66 (4):1113-1121.
doi: 10.1093/jxb/eru534 pmid: 25628331
[3] Wan Y, Yan Y H, Yang W Y, et al. Responses of root growth and nitrogen transfer metabolism to uniconazole, a growth retardant, during the seedling stage of soybean under relay strip intercropping system. Communications in Soil Science and Plant Analysis, 2013, 44(22):3267-3280.
doi: 10.1080/00103624.2013.840838
[4] Yan Y H, Gong W Z, Yang W Y, et al. Seed treatment with uniconazole powder improves soybean seedling growth under shading by corn in relay strip intercropping system. Plant Production Science, 2010, 13(4):367-374.
doi: 10.1626/pps.13.367
[5] Yan Y H, Wan Y, Liu W G, et al. Influence of seed treatment with uniconazole powder on soybean growth, photosynthesis, dry matter accumulation after flowering and yield in relay strip intercropping system. Plant Production Science, 2015, 18(3):295-301.
doi: 10.1626/pps.18.295
[6] Zou J N, Jin X J, Zhang Y X, et al. Effects of melatonin on photosynthesis and soybean seed growth during grain filling under drought stress. Photosynthetica, 2019, 57(2):512-520.
doi: 10.32615/ps.2019.066
[7] Nawaz K, Chaudhary R, Sarwar A, et al. Melatonin as master regulator in plant growth, development and stress alleviator for sustainable agricultural production: Current status and future perspectives. Sustainability, 2020, 13(1):294.
doi: 10.3390/su13010294
[8] 韩毅强, 石英, 高亚梅, 等. 赤霉素及烯效唑对大豆形态、光合生理及产量的影响. 中国油料作物学报, 2018, 40(6):820-827.
[9] Zhang M C, Duan L S, Zhai Z X, et al. Effects of plant growth regulators on water deficit-induced yield loss in soybean. Proceedings of the 4th International Crop Science Congress, Brisbane, Australia, 2004.
[10] 宫香伟, 刘春娟, 冯乃杰, 等. S3307和DTA-6对大豆不同冠层叶片光合特性及产量的影响. 植物生理学报, 2017, 53(10):1867-1876.
[11] 李宇, 赵松义, 聂明建, 等. 不同温度下烯效唑对烟草成苗素质的影响. 作物研究, 2014, 28(3):280-283.
[12] 李合生. 植物生理学实验指导. 北京: 高等教育出版社, 2000.
[13] 张志良, 瞿伟菁. 植物生理学实验指导. 北京: 高等教育出版社, 2003.
[14] 刘丽琴, 张永清, 李鑫, 等. 烯效唑浸种对红小豆种子萌发及幼苗生长的影响. 江苏农业科学, 2017, 45(3):64-70.
[15] Yamaguchi S, Kamiya Y, Sun T P. Distinct cell‐specific expression patterns of early and late gibberellin biosynthetic genes during Arabidopsis seed germination. The Plant Journal, 2001, 28(4):443-453.
doi: 10.1046/j.1365-313X.2001.01168.x
[16] 陈贤, 杨勇, 刘凤权. 植物褪黑素的研究进展. 江苏农业科学, 2020, 48(24):17-24.
[17] Verbančič J, Lunn J E, Stitt M, et al. Carbon supply and the regulation of cell wall synthesis. Molecular Plant, 2018, 11(1):75-94.
doi: S1674-2052(17)30303-9 pmid: 29054565
[18] Mishra B S, Sharma M, Laxmi A. Role of sugar and auxin crosstalk in plant growth and development. Physiologia Plantarum, 2022, 174(1):e13546.
doi: 10.1111/ppl.v174.1
[19] Han Y Q, Gao Y M, Shi Y, et al. Genome-wide transcriptome profiling reveals the mechanism of the effects of uniconazole on root development in Glycine Max. Journal of Plant Biology, 2017, 60(4):387-403.
doi: 10.1007/s12374-017-0028-9
[20] Huangfu L, Zhang Z, Zhou Y, et al. Integrated physiological, metabolomic and transcriptomic analyses provide insights into the roles of exogenous melatonin in promoting rice seed germination under salt stress. Plant Growth Regulation, 2021, 95(1):19-31.
doi: 10.1007/s10725-021-00721-9
[21] Li G F, Ma J J, Tan M, et al. Transcriptome analysis reveals the effects of sugar metabolism and auxin and cytokinin signaling pathways on root growth and development of grafted apple. BMC Genomics, 2016, 17(1):1-17.
[22] Eljebbawi A, Guerrero Y D C R, Dunand C, et al. Highlighting reactive oxygen species as multitaskers in root development. Iscience, 2021, 24(1):101978.
doi: 10.1016/j.isci.2020.101978
[23] Lee Z H, Hirakawa T, Yamaguchi N, et al. The roles of plant hormones and their interactions with regulatory genes in determining meristem activity. International Journal of Molecular Sciences, 2019, 20(16):4065.
doi: 10.3390/ijms20164065
[24] Mase K, Tsukagoshi H. Reactive oxygen species link gene regulatory networks during Arabidopsis root development. Frontiers in Plant Science, 2021, 12:660274.
doi: 10.3389/fpls.2021.660274
[25] Smeekens S, Ma J, Hanson J, et al. Sugar signals and molecular networks controlling plant growth. Current Opinion in Plant Biology, 2010, 13(3):273-278.
doi: 10.1016/j.pbi.2009.12.002
[26] Poór P. Effects of salicylic acid on the metabolism of mitochondrial reactive oxygen species in plants. Biomolecules, 2020, 10(2):341.
doi: 10.3390/biom10020341
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