Crops ›› 2025, Vol. 41 ›› Issue (1): 133-138.doi: 10.16035/j.issn.1001-7283.2025.01.016

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Analysis of Cell Structure Characteristics and Polygalacturonase Content Changes in Sugarcane Leaves Abscission Zone at Maturity Stage

Ou Kewei(), Lu Yefei, Nong Zemei, Zhu Pengjin, Pang Xinhua, Song Qiqi, Lü Ping()   

  1. Guangxi Subtropical Crops Research Institute, Nanning 530001, Guangxi, China
  • Received:2023-05-10 Revised:2023-10-30 Online:2025-02-15 Published:2025-02-12

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Abstract:

In order to screen excellent sugarcane varieties with strong defoliation, the leaves at different positions of three sugarcane varieties (lines) with different defoliation were used as materials to observe the structure of detached cells of sugarcane leaves and determine the polygalacturoncase (PG) content in sugarcane leaves, middle leaf sheath and basal leaf sheath. The results showed that the decomposition degree of leaf sheath abscission tissue cells was the highest in easily defoliated sugarcane. The PG content in the basal part of +5 leaf sheath and +8 leaf sheath of easy-defoliation sugarcane were significantly higher than that of hard-defoliation sugarcane at three different mature stages. The PG content in the basal part of +8 leaf sheath at the early maturity stage of sugarcane was positively correlated with the cleavage degree of the cells in the corresponding leaf abscission zone. The results of grey correlation analysis showed that the content of PG in the +8 leaf, the middle of the +5 leaf sheath and the base of the +8 leaf sheath had a great correlation with the natural defoliation rate of sugarcane leaves. Combined with the measured PG content in each period, the PG content in the base of the +8 leaf sheath at the early maturity can provide reference for screening of sugarcane varieties with good defoliation traits.

Key words: Sugarcane, Defoliation rate, Abscission zone, Polygalacturonase

Table 1

Natural defoliation rate of three varieties (lines) sugarcane %"

品种（系）
Variety (line)		 脱叶率Defoliation rate
成熟早期Early mature stage 成熟中期Mid-mature stage 成熟后期Late mature stage
ROC22 8.98±0.02Bb 15.10±0.02Bb 33.24±0.03Bb
GT47 0.00±0.00Bb 0.00±0.00Cc 5.01±0.04Cc
LZ02-169 49.49±0.05Aa 58.67±0.03Aa 69.20±0.26Aa

Fig.1

Cells morphology of abscission zone of LZ02-169, ROC22 and GT47 The position marked by the arrow is the abscission zone."

Fig.2

Comparison of polygalacturonase content among different varieties (lines) of sugarcane during maturation (a): Early maturation; (b): Mid-maturation; (c): Late maturation. Different lowercase letters indicate significant difference at 5% level, different capital letters indicate significant difference at 1% level."

Table 2

Association of PG contents of different positions with natural leaf defoliation rates"

因子Factor 关联系数Correlation coefficient 排序Ranking
叶片（+8）Leaf (+8) 0.3833 1
叶鞘中部（+5）Leaf sheath central (+5) 0.3802 2
叶鞘基部（+8）Leaf sheath base (+8) 0.3577 3
叶鞘基部（+5）Leaf sheath base (+5) 0.3248 4
叶鞘基部（+2）Leaf sheath base (+2) 0.2998 5
叶片（+2）Leaf (+2) 0.2936 6
叶鞘中部（+8）Leaf sheath central (+8) 0.2881 7
叶鞘中部（+2）Leaf sheath central (+2) 0.2880 8
叶片（+5）Leaf (+5) 0.2520 9
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