Crops ›› 2017, Vol. 33 ›› Issue (4): 150-154.doi: 10.16035/j.issn.1001-7283.2017.04.025

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Preliminary Study on the Signal Molecule of Phytophthora infestans Responsed by Antagonistic Actinomycetes Strain Sy11

Wang Youyou1,Jiang Jizhi1,Tian Huiyao1,Li Ming1,Wang Xuening1,Wu Yanqing2,Sun Han2   

  1. 1 College of Life Sciences,Hebei University,Baoding 071002,Hebei,China
    2 Department of Biochemistry,Baoding University,Baoding 071000,Hebei,China
  • Received:2017-04-18 Revised:2017-07-12 Online:2017-08-15 Published:2018-08-26
  • Contact: Jizhi Jiang

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Abstract:

In order to identify the potential of Sy11 strain in preventing potato late blight and the response between actinomycetes Sy11 strain and Phytophthora infestans, the punch method and detached tissue method were adopted to evaluate the inhibition of Sy11 strain on P. infestans and the prevention of late blight on potato tuber slices and leaves. The dual-culture method and cardboard isolation method were used to affirm the response between both strains and to clear the solubility and stability of signal molecule produced by P. infestans to Sy11 strain response. The results indicated that inhibitory rates of Sy11 strain living body and mycelium liquid to P. infestans mycelium growth were 89.7% and 82.5%, respectively. The relative protection ratios of Sy11 mycelium liquid on both in vitro tuber slices and detached leaves were higher than 85.0%. In addition, it was verified that under the induction of P. infestans only, Sy11 strain could secrete antiomycetes substances and its inhibitory rates could reach up to 76.5%. Signal molecule from P. infestans responded by Sy11 strain soluble in methanol and tolerant 80℃ temperature. These results not only indicate the prominent potential of Sy11 strain on prevention potato late blight, but also provide a reference for further separation and purification of P. infestans signal molecule as well as the clarification of mechanism of response and induction.

Key words: Streptomyces microflavus, Phytophthora infestans, Inhibition, Induction, Signal molecule

Table 1

The inhibition of Sy11 strain on P. infestans and prevention effect of late blight on potato detached tissues %"

菌丝体生长抑制率Inhibition rate on mycelium growth 对照Control 0b
菌活体Living body 89.7a
菌液Mycelium liquid 82.5a
发酵液Fermented liquid 0b
孢子萌发抑制率Inhibition rate of spore germination 对照Control 0b
游动孢子释放Zoospore release 86.2a
孢子囊直接萌发Sporangium direct germination 82.3a
游动孢子萌发Zoospore germination 78.9a
离体组织相对保护率Relative protection ratio on detached tissues 对照Control 0b
块茎Tuber 85.4a
叶片Leaf 88.7a

Fig.1

Effects of different temperature on activity of P. infestans signal molecular"

Fig.2

Effects of different pH values on activity of P. infestans signal molecular"

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