Crops ›› 2016, Vol. 32 ›› Issue (4): 162-166.doi: 10.16035/j.issn.1001-7283.2016.04.028

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Comparison of Three Antagonistic Bacteria against Phytophthora infestans in Potato

Zhang Yahui,Jiang Jizhi,Wang Beibei,Li Ying,Wan Anqi,Sha Haitian   

  1. College of Life Sciences,Hebei University,Baoding 071002,Hebei,China
  • Received:2016-03-04 Revised:2016-05-12 Online:2016-08-15 Published:2018-08-26
  • Contact: Jizhi Jiang

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Abstract:

Sy11, M15 and A5295 are antagonistic strains of Phytophthora infestans, screened in previously experiments in our lab. In order to clear which is the most potential strain, the partial inhibitory characteristics of the three strains on P.infestans against and control efficiency of late blight on potato tuber slices were compared with dual-culture, punch method, and potato tuber slices, respectively. The results showed that both Sy11 and M15 were evident better than A5295 in suppression mycelial growth of P. infestans and spore germination, and three strains had no significant differences in control late blight on potato tuber slices. But Sy11 was stronger than M15 in restraining sporangia formation and zoospores germination, the inhibition rates of former were 68.9% and 71.3%, respectively, that of later were 56.7% and 67.3%, respectively, there were significant differences between Sy11 and M15. These findings showed that Sy11 strain might have the most potential in control potato late blight in future.

Key words: Phytophthora infestans, Potato, Antagonists, Inhibition effect, Detached tissue disease prevention

Fig.1

Inhibition of three antagonistic strains to P. infestans"

Table 1

Effects of three antagonistic bacterial on sporangium formation of P.infestans"

菌株Strains 孢子囊形成量(个/cm2) Sporangium formation
Sy11 125aA
M15 174bB
A5295 386cC
CK 402cC

Fig.2

Effects of three antagonistic bacterial broth on zoospore release,germination and sporangia direct germination of P.infestans Different small letters indicate significant different at 0.05 level"

Fig.3

Effects of antagonistic bacteria liquid on the mycelium morphology of the P.infestans"

Fig.4

Protection efficiency of three antagonistic bacteria broth on potato tuber slices against P.infestans"

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