Crops ›› 2019, Vol. 35 ›› Issue (6): 195-202.doi: 10.16035/j.issn.1001-7283.2019.06.031

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Effects of Melatonin Soaking on Germination of Soybean Seeds under Saline-Alkali Stress

Wang Mingyao,Cao Liang,Yu Qi,Zou Jingnan,He Songyu,Qin Bin,Wang Mengxue,Zhang Yuxian   

  1. College of Agronomy, Heilongjiang Bayi Agricultural University, Daqing 163319, Heilongjiang, China
  • Received:2019-06-24 Revised:2019-10-16 Online:2019-12-15 Published:2019-12-11
  • Contact: Yuxian Zhang

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Abstract:

In order to study the effects of melatonin soaking on the germination of soybean seeds under salt-alkali stress, the soybean variety Kenfeng 16 was used as experimental material to study the effects of 100μmol/L melatonin soaked on the seeds germination of soybean under different concentrations of mixed saline-alkali stress. The results showed that compared with the control, with the increase of concentration, the saline-alkali stress continuously reduced the germination rate, germination index, bud fresh weight and dry weight of soybean seeds, and the antioxidant enzymes superoxide dismutase, peroxidase, catalase, glutathione reductase, glutathione peroxidase activities rised first and then decreased. The activity of APX increased continuously. The content of soluble sugar, soluble protein and proline osmotic adjustment substances increased first and then decreased. The content of membrane lipid peroxidation products malonaldehyde (MDA), H2O2, and $\mathop{{O}}_{2}^{{\mathop{}_{\ .}^{-}}}$ rate of production was on the rise. Melatonin soaking could increase the germination rate, germination index, bud fresh weight, bud dry weight and antioxidant enzymes activity, as well as the content of osmotic adjustment substances, and decrease the content of MDA, H2O2 and $\mathop{{O}}_{2}^{{\mathop{}_{\ .}^{-}}}$ production rate under different concentrations of saline-alkali stress. In summary, melatonin soaking can alleviate the inhibition of soybean seed germination under different concentrations of saline-alkali stress, and can increase the activity of antioxidant enzymes and osmotic adjustment substances, and reduce the degree of lipid peroxidation on membrane.

Key words: Saline-alkali stress, Soybean, Melatonin, Seed germination

Fig.1

Effects of melatonin on the germination rate of soybean seeds under saline-alkali stress Different lowercase letters indicate significant difference (P<0.05), the same below"

Fig.2

Effects of melatonin on germination index of soybean seeds under saline-alkali stress"

Fig.3

Effects of melatonin on the relative salt injury rate of soybean seeds under saline-alkali stress"

Fig.4

Effects of melatonin on antioxidant enzyme activities of soybean seeds under saline-alkali stress"

Fig.5

Effects of melatonin on the activity of GR and GPX of soybean seeds under saline-alkali stress"

Fig.6

Effects of melatonin on osmotic adjustment substance content and membrane lipid peroxidation of soybean seeds under saline-alkali stress"

Table 1

Effects of melatonin on bud length,bud fresh weight and bud dry weight of soybean seed under saline-alkali stress"

处理
Treatment		 芽长(cm)
Bud length		 芽鲜重(g)
Bud fresh weight		 芽干重(g)
Bud dry weight
CK0 9.15±0.83a 0.85±0.06a 0.059±0.001a
MCK0 9.18±1.63a 0.89±0.05a 0.062±0.003a
CK25 7.86±2.72ab 0.59±0.08bc 0.041±0.005bc
MCK25 8.07±1.53ab 0.68±0.10b 0.047±0.008b
CK50 6.42±0.67b 0.45±0.01c 0.031±0.002c
MCK50 7.01±0.51b 0.56±0.05bc 0.039±0.006bc
CK100 4.79±1.25cd 0.30±0.02de 0.021±0.004e
MCK100 5.58±0.56c 0.36±0.03d 0.025±0.001d
CK200 3.25±0.78d 0.21±0.01f 0.015±0.002f
MCK200 3.30±1.53d 0.26±0.03e 0.018±0.001ef

Table 2

Correlation coefficient between germination rate and related physiological and biochemical indexes of soybean seeds"

指标Index 发芽率
Germination
rate		 SOD活性
SOD
activity		 POD活性
POD
activity		 CAT活性
CAT
activity		 APX活性
APX
activity		 MDA含量
MDA
content		 H2O2含量
H2O2
content		 脯氨酸含量
Proline content		 可溶性糖含量
Soluble sugar content		 可溶性蛋白含量
Soluble protein content
发芽率Germination rate -1
SOD活性SOD activity -0.349 -1
POD活性POD activity -0.322 -0.296 1
CAT活性CAT activity -0.089 -0.547 0.933** 1
APX活性APX activity -0.847** -0.325 0.331 0.061 -1
MDA含量MDA content -0.973** -0.469 0.254 0.027 -0.773** -1
H2O2含量H2O2content -0.963** -0.173 0.426 0.255 -0.758* -0.935** -1
脯氨酸含量Proline content -0.265 -0.961** 0.463 0.658* -0.242 -0.385 -0.106 1
可溶性糖含量
Soluble sugar content		 -0.266 -0.962** 0.463 0.659* -0.243 -0.386 -0.106 1.000** 1
可溶性蛋白含量
Soluble protein content		 -0.307 -0.334 0.949** 0.893** -0.404 -0.226 -0.384 0.506 0.505 1
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