Crops ›› 2021, Vol. 37 ›› Issue (6): 46-50.doi: 10.16035/j.issn.1001-7283.2021.06.007

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Observation on Pollen Morphology of F-Type Male Sterile Lines and SQ-1 Induced Sterile Plants in Wheat by Scanning Electron Microscope

Ren Wenbin(), Wang Qian, Wu Cuicui, Xie Sangang   

  1. Institute of Cotton Research, Shanxi Agricultural University (Shanxi Academy of Agricultural Sciences), Yuncheng 044000, Shanxi, China
  • Received:2020-12-21 Revised:2021-03-01 Online:2021-12-15 Published:2021-12-16

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Abstract:

The morphology and structure of pollen grains of F-type male sterile lines and SQ-1 induced male sterile plants were observed by scanning electron microscope. By comparing the characteristics of pollen grain size, shape and germination aperture cover. The results showed that the polar axis length of pollen grains reached extremely significant level among normal development, SQ-1 induced and F-type male sterile lines. The pollen grains of SQ-1 induced sterile plants and F-type male sterile lines showed typical irregular abortion shape, and the germination aperture cover were sunken in different degrees. The shedding of germinating aperture cover was found at the same time.

Key words: Wheat, F-type male sterile line, Pollen grain, Germinal aperture

Table 1

Comparison of pollen grains of F-type male sterile lines, SQ-1 induced and normal development"

类型
Type		 花粉粒大小Pollen grain size (μm) 萌发孔大小
Germinal aperture
size (μm)		 萌发孔孔盖特征
Characteristic of germinal aperture cap
极轴长
Polar axis length		 赤道轴长
Equatorial axis length
正常发育
Normal development		 65.02±5.81aA 50.13±4.16abA （8.60±0.68）×（8.18±0.54） 圆润饱满挺直,向上凸起,高于孔环
舜麦1718 Shunmai 1718
（SQ-1诱导）		 46.53±3.95cC 40.40±3.18cB （8.04±0.56）×（7.22±0.51） 向下凹陷,低于孔环
晋麦84 Jinmai 84
（SQ-1诱导）		 43.81±3.55cC 39.62±2.82cB （7.88±0.58）×（7.05±0.61） 周围向下凹陷,孔盖凸起,高度平行于孔环
FA37-1 53.92±4.76bB 49.54±3.35bA （8.16±0.65）×（7.13±0.49） 向下凹陷,低于孔环
FA99-2 56.93±5.12bB 47.44±3.62bA （8.35±0.71）×（7.26±0.58） 向下凹陷,低于孔环
FA128-2 54.84±4.64bB 48.54±3.17bA （8.28±0.63）×（6.94±0.50） 向下凹陷,低于孔环

Fig.1

The morphological characteristics of the normal pollen grains and germinal aperture a and b represent the morphological characteristics of the pollen grains at 100× and 500×, respectively, c represents the morphological characteristics of germinal aperture at 10000×. The same below"

Fig.2

The morphological characteristics of the pollen grains and germinal aperture of Shunmai 1718 and Jinmai 84 induced by SQ-1"

Fig.3

The morphological characteristics of the pollen grains and germinal aperture of FA37-1, FA99-2, and FA128-2 male sterile lines"

Fig.4

The cap of germinal aperture felling off morphological characteristics of the normal pollen grains a represents the morphological characteristics of the pollen grains at 500×, b represents the morphological characteristics of germinal aperture at 5000×"

[1] 滕文涛, 曹靖生, 陈彦惠, 等. 十年来中国玉米杂种优势群及其模式变化的分析. 中国农业科学, 2004, 37(12):1804-1811.
[2] 王志新, 郭泰, 赵丽梅, 等. 大豆杂交种杂种优势分析. 中国农学通报, 2010, 26(15):185-189.
[3] 张启发. 中国科学家阐明BoroⅡ型水稻细胞质雄性不育和育性恢复的分子机理. 分子植物育种, 2006, 4(4):451-452.
[4] 刘琪迩, 杜坤, 王幼平. 油菜细胞质雄性不育与育性恢复机理的研究进展. 生物技术通报, 2015, 31(9):15-22.
doi: 10.13560/j.cnki.biotech.bull.1985.2015.09.004
[5] 牛国阳, 陈庆富. 小麦杂种优势利用的现状与展望. 种子, 2008, 27(3):44-47.
[6] 赵昌平. 中国杂交小麦研究现状与趋势. 中国农业科技导报, 2010, 12(2):5-8.
[7] 李传友, 孙兰珍. 普通小麦T型、V型和K型细胞质雄性不育系花粉败育机理的细胞学研究. 华北农学报, 1996, 11(2):1-8.
[8] 钱焕焕, 罗钊, 李艳丽, 等. YS型小麦温敏不育系A731雄性败育的细胞学研究. 麦类作物学报, 2012, 32(6):1026-1031.
[9] 郑爱泉. 化学杂交剂诱导小麦雄性不育研究进展. 贵州农业科学, 2014, 42(8):17-22.
[10] 桑伟, 韩新年, 田笑明, 等. 小麦杂种优势的利用途径及研究进展. 种子, 2005, 24(5):50-54.
[11] 贺晓敏, 周美兰, 余传启, 等. 温敏雄性不育小麦BNS366花粉败育的细胞学观察. 麦类作物学报, 2014, 34(4):460-466.
[12] 李罗江, 茹振刚, 高庆荣, 等. BNS小麦的雄性不育性及其温光特性. 中国农业科学, 2009, 42(9):3019-3027.
[13] 刘保申, 孙其信, 高庆荣, 等. K型小麦细胞质雄性不育系育性恢复基因的SSR分子标记分析. 中国农业科学, 2002, 35(4):354-358.
[14] 温辉芹, 马惠英, 裴自友, 等. F型小麦不育系育性与杂种优势研究. 山西农业科学, 2017, 45(8):1219-1224.
[15] 王坤杨, 张伟, 张双喜, 等. 化学杀雄剂SQ-1和阿拉伯葡聚糖蛋白对小麦品种间杂交及远缘杂交成胚率的影响. 中国农业科学, 2016, 49(24):4824-4832.
[16] 卢纹岱. SPSS for windows 统计分析. 北京: 电子工业出版社, 2000.
[17] 陈林, 吴迪, 白文明, 等. F型小麦雄性不育系测恢研究及杂种优势分析. 麦类作物学报, 2016, 36(4):420-425.
[18] 任永康, 牛瑜琦, 逯成芳, 等. F型小麦雄性不育系与普通小麦杂交组合选配探讨. 山西农业科学, 2019, 47(1):20-23.
[19] 张思妮, 马小飞, 张红霞, 等. F型小麦雄性不育系花粉败育和恢保关系研究. 西北植物学报, 2016, 36(4):667-673.
[20] 赵卜, 张向展, 郑炜君, 等. F型小麦雄性不育系小孢子发育的细胞学观察. 麦类作物学报, 2015, 35(7):918-925.
[21] 姚雅琴, 唐群, 张英利, 等. 小麦花粉萌发孔与雄性不育关系初探. 电子显微学报, 2006, 25(5):426-430.
[22] 柳娜, 杨文雄, 王世红, 等. 化学杀雄剂SQ-1诱导春小麦雄性不育的效果和对农艺性状的影响. 作物杂志, 2014(1):23-25.
[23] 李国强, 逯腊虎, 武计萍, 等. 化学杂交剂SQ-1诱导小麦雄性不育的效果及F1代杂种优势表现. 麦类作物学报, 2011, 31(4):637-641.
[24] 柳娜, 杨文雄, 王世红, 等. 化学杀雄剂SQ-1对春小麦的化学杀雄效果初报. 甘肃农业科技, 2017(10):5-7.
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