Crops ›› 2023, Vol. 39 ›› Issue (5): 280-284.doi: 10.16035/j.issn.1001-7283.2023.05.039

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Isolation and Identification of Endophytes from Sunflower Seeds

Zhu Kongyan1(), Han Shengcai2, Zhao Rong1, Wen Yujie1, Hu Haochi1, Qiao Yimin3, Lu Jiafeng1, Cao Kai1, Xu Zhenghan1, Bao Haizhu1(), Gao Julin1()   

  1. 1College of Agriculture, Inner Mongolia Agricultural University, Hohhot 010019, Inner Mongolia, China
    2College of Horticulture and Plant Protection, Inner Mongolia Agricultural University, Hohhot 010019, Inner Mongolia, China
    3Inner Mongolia Hangjinhouqi Yimin Seed Co., Ltd., Bayannur 015400, Inner Mongolia, China
  • Received:2022-10-28 Revised:2023-03-22 Online:2023-10-15 Published:2023-10-16

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Abstract:

In order to provide a basis for biological control of sunflower Sclerotinia, the biocontrol bacteria with antagonistic effects on Sclerotinia sclerotiorum causing sunflower Sclerotinia were screened. The endophytes were isolated and identified from the seeds of nine genotypes of sunflower inbred lines, and the biocontrol strains with resistance to S.sclerotiorum were screened. Through bacterial genomic DNA extraction, PCR amplification, construction of 16S rDNA gene sequence alignment identification, and antagonistic activity detection by plate confrontation method, the endophytic bacteria isolated from the seeds of sunflower material 217014 was Bacillus subtilis and was named Y116. Y116 could effectively inhibit the growth of S.sclerotiorum mycelium and the formation of Sclerotinia, and had a biocontrol effects on the S.sclerotiorum that caused sunflower Sclerotinia.

Key words: Sunflower, Endophyte, Bacillus subtilis, Antagonizing activity

Table 1

Isolation results of endophytic bacteria from nine genotypes of sunflower materials"

项目Item 202002 202004 202007 202011 202014 217002 217012 217013 217014 合计Total
菌种数Number of bacteria strains 4 15 26 23 15 17 7 7 10 124

Fig.1

The growth of sunflower pots treated with strains Y62, Y96 and Y116"

Fig.2

Phylogenetic tree of 16S rDNA of endophytic strain Y116 from sunflower"

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