Crops ›› 2024, Vol. 40 ›› Issue (2): 234-241.doi: 10.16035/j.issn.1001-7283.2024.02.029

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Effects of Seed Soaking with Uniconazole on Agronomic and Physiological Characteristics of Quinoa under Saline-Alkali Stress

He Jiamin1(), Zhang Yongqing1,2(), Zhang Meng1, Liang Ping1, Wang Dan1, Yan Fanfan1   

  1. 1College of Life Sciences, Shanxi Normal University, Taiyuan 030000, Shanxi, China
    2College of Geography, Shanxi Normal University, Taiyuan 030000, Shanxi, China
  • Received:2023-05-26 Revised:2023-08-01 Online:2024-04-15 Published:2024-04-15

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Abstract:

In order to investigate the mitigative effects of seed soaking with uniconazole on the growth of quinoa seedlings under saline-alkali stress, Longli 1 was used as experimental material, the effects of seed soaking with uniconazole (0, 15, 30, 45 and 60 mg/L) at different concentrations on the agronomic characteristics and physiological parameters of quinoa at seedling stage (28 d after sowing) under different saline-alkali stress (CK 0 g/kg, mild saline-alkali 3 g/kg, moderate saline-alkali 5 g/kg, heavy saline-alkali 7 g/kg) were studied by pot experiment. The results showed that the osmotic substance contents, antioxidant enzyme activities and root activity of quinoa seedlings were increased under low salinity (3 g/kg), so as to cope with saline-alkali environment and alleviate oxidative damage. The root-shoot ratio and seedling strength index reached the maximum under moderate saline-alkali (5 g/kg) treatment. The agronomic parameters, photosynthetic parameters, osmotic substance contents, antioxidant enzyme activities and root activity of quinoa seedlings decreased significantly under high salinity, and malondialdehyde content reached the maximum under heavy saline-alkali (7 g/kg), the results indicated that the growth of quinoa seedlings was significantly affected by salinity. At the same saline-alkali level, the root-shoot ratio, seedling strength index, chlorophyll content, osmotic substance contents, antioxidant enzyme activities and root activity of quinoa seedlings were significantly increased after seed soaking with low concentration of uniconazole, the content of malondialdehyde decreased, and the effect was the most significant when the concentration of seed soaking was 15 mg/L.

Key words: Saline-alkali stress, Uniconazole, Quinoa, Seedling strength index, Physiological characteristics

Table 1

Effects of seed soaking with uniconazole on the growth parameters of quinoa seedlings under saline-alkali stress"

盐碱处理
Saline-alkali treatment (g/kg)		 烯效唑处理
Uniconazole treatment (mg/L)		 株高
Plant height (cm)		 茎粗
Stem diameter (mm)		 主根长
Main root length (cm)		 生物量（g/株）
Biomass (g/plant)
0 0 20.13±0.16Aa 1.56±0.03Ca 17.05±0.21Dc 2.76±0.03Ba
15 17.65±0.12Ba 1.96±0.04Aa 26.37±0.17Ac 2.96±0.02Aa
30 16.12±0.09Ca 1.73±0.03Ba 24.23±0.23Bc 2.74±0.04Ba
45 14.94±0.10Da 1.63±0.03Ba 20.51±0.20Cc 2.59±0.03Ca
60 13.12±0.08Ea 1.43±0.02Da 15.11±0.17Ec 2.51±0.01Ca
3 0 15.83±0.09Ab 1.41±0.02Db 21.47±0.28Da 2.52±0.02Bb
15 14.54±0.08Bb 1.78±0.05Ab 31.51±0.31Aa 2.76±0.03Ab
30 13.27±0.12Cb 1.65±0.03Bb 28.61±0.26Ba 2.51±0.02Bb
45 12.13±0.07Db 1.51±0.04Cb 23.85±0.24Ca 2.35±0.04Cb
60 11.03±0.05Eb 1.32±0.04Eb 17.15±0.15Ea 2.12±0.01Db
5 0 12.87±0.11Ac 1.29±0.06Cc 19.41±0.16Cb 2.17±0.02Bc
15 11.41±0.09Bc 1.51±0.02Ac 28.32±0.13Ab 2.36±0.03Ac
30 10.02±0.05Cc 1.38±0.05Bc 26.13±0.17Bb 2.20±0.02Bc
45 9.26±0.08Dc 1.36±0.02Bc 20.93±0.16Cb 2.07±0.02Cc
60 9.27±0.04Dc 1.14±0.02Dc 16.45±0.15Db 1.83±0.01Dc
7 0 9.87±0.08Ad 1.12±0.03Cd 10.26±0.14Dd 1.72±0.03Bd
15 8.65±0.09Bd 1.38±0.02Ad 16.85±0.21Ad 1.88±0.03Ad
30 7.42±0.12Cd 1.24±0.04Bd 14.46±0.12Bd 1.73±0.01Bd
45 7.31±0.06Cd 1.13±0.06Cd 12.12±0.11Cd 1.69±0.02Bd
60 6.17±0.04Dd 1.01±0.02Dd 8.01±0.12Ed 1.58±0.01Cd

Fig.1

Effects of seed soaking with uniconazole on root-shoot ratio and seedling strength index in quinoa seedlings under saline-alkali stress Different capital letters indicate significant differences among different seed soaking concentrations under the same saline-alkali condition (P < 0.05), and different lowercase letters indicate significant differences among different saline-alkali conditions under the same seed soaking concentration (P < 0.05), the same below."

Fig.2

Effects of seed soaking with uniconazole on the contents of chlorophyll and physiological index in quinoa seedlings under saline-alkali stress"

Fig.3

Effects of seed soaking with uniconazole on POD and SOD activities in quinoa seedlings under saline-alkali stress"

Fig.4

Effects of seed soaking with uniconazole on the contents of MDA in quinoa seedlings under saline-alkali stress"

Fig.5

Effects of seed soaking with uniconazole on root activity in quinoa seedlings under saline-alkali stress"

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