Crops ›› 2024, Vol. 40 ›› Issue (4): 269-276.doi: 10.16035/j.issn.1001-7283.2024.04.035

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Effects of Different Sowing and Fertilization Methods on Contents of Mineral Elements, Chlorophyll and Active Enzymes of Highland Barley

Jiang Di1,2,3(), Xie Huichun1,2,3(), Jin Xionglian1,2,3, Mao Xiaoning1,2,3, Du Shaobo1,2,3   

  1. 1School of Life Sciences, Qinghai Normal University, Xining 810008, Qinghai, China
    2Key Laboratory of Biodiversity Formation Mechanism and Comprehensive Utilization on the Qinghai Tibet Plateau, Xining 810008, Qinghai, China
    3Key Laboratory of Medicinal Animal and Plant Resources on the Qinghai Tibet Plateau, Xining 810008, Qinghai, China
  • Received:2023-06-30 Revised:2023-09-15 Online:2024-08-15 Published:2024-08-14

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Abstract:

In order to explore the highland barley varieties suitable for planting in Huangzhong county, Qinghai province and the optimal methods of sowing and fertilization, five highland barley varieties (Beiqing 8, Beiqing 9, Kunlun 14, Kunlun 17 and Dulihuang) were selected for variety comparison test. According to the differences of seedling yield, chlorophyll, mineral elements and active enzyme contents, excellent highland barley varieties were selected, and different sowing (row spacing and sowing amount) and fertilization (type and fertilization amount) methods were set for Beiqing 9, the effects of different sowing and fertilization methods on the contents of mineral elements, chlorophyll, superoxide dismutase (SOD) and cytochrome oxidase were studied. The results showed that the contents of mineral elements in Kunlun 17 and Beiqing 9 were higher, among which the seedling yield of Kunlun 17 was the highest, and the contents of chlorophyll and cytochrome oxidase of Beiqing 9 were the highest. The contents of mineral elements, SOD and cytochrome oxidase in Beiqing 9 increased significantly with increasing of seeding amount and row spacing, the contents of chlorophyll and SOD increased significantly with the increasing of N+P and N fertilizers, and the contents of cytochrome oxidase increased significantly with the increasing of P fertilizer; The correlation between most mineral elements in highland barley reached a very significant level.

Key words: Highland barley, Mineral element, Chlorophyll, Superoxide dismutase, Cytochrome oxidase

Table 1

Regression equation and correlation coefficients of different elements"

元素
Element		 回归方程
Regression equation		 相关系数
Correlation coefficient
Ca A=138 101.3248c+34.7131 0.99 999
Fe A=2674.9570c-7.8182 0.99 968
Mg A=66 960.5575c+1.0606 0.99 991
Al A=14 801.3963c-2.5101 0.99 930
Na A=19 598.0383c-17.7411 0.99 904
Zn A=5787.7268c-22.0526 0.99 977
Cu A=45 789.9780c-2.7701 0.99 989
Mn A=18 502.8647c-0.3745 0.99 985

Table 2

Contents of eight mineral elements in different highland barley varieties mg/g"

品种Variety Ca Fe Mg Al Na Zn Cu Mn
北青8号Beiqing 8 0.715 0.461 1.243 0.044 0.325 0.071 0.008 0.052
北青9号Beiqing 9 1.359 0.873 1.774 0.081 0.413 0.102 0.013 0.058
昆仑14号Kunlun 14 0.862 0.475 1.346 0.040 0.589 0.071 0.004 0.036
昆仑17号Kunlun 17 1.074 0.749 1.074 0.136 0.802 0.091 0.009 0.054
肚里黄Dulihuang 1.140 0.901 1.457 0.066 0.035 0.089 0.006 0.056

Fig.1

Seedling yield and contents of chlorophyll, SOD and cytochrome oxidase of different highland barley varieties Different lowercase letters show extremely significant difference at 0.01 level, the same below."

Table 3

Average contents of eight mineral elements in Beiqing 9 under different treatments mg/g"

处理Treatment Ca Fe Mg Al Na Zn Cu Mn
H1B1 0.752 0.127 1.143 0.0476 0.452 0.149 0.083 0.072
H1B2 0.761 0.115 1.451 0.0129 0.660 0.104 0.012 0.061
H1B3 0.709 0.339 1.376 0.0651 0.516 0.049 0.020 0.029
H1B4 0.730 0.099 1.193 0.0162 0.877 0.054 0.009 0.040
H1B5 0.973 0.091 0.879 0.0168 0.849 0.070 0.014 0.084
H2B1 0.996 0.258 0.894 0.0224 0.771 0.113 0.020 0.087
H2B2 0.806 0.091 1.585 0.0163 0.432 0.094 0.020 0.078
H2B3 0.828 0.045 0.798 0.0080 0.643 0.055 0.010 0.047
H2B4 0.961 0.054 0.863 0.0126 0.638 0.053 0.008 0.042
H2B5 1.324 0.148 0.926 0.0254 0.772 0.127 0.043 0.102
H3B1 0.866 0.139 1.008 0.0090 0.431 0.099 0.018 0.084
H3B2 0.696 0.209 1.411 0.0303 0.435 0.141 0.014 0.263
H3B3 0.936 0.113 1.115 0.0747 0.541 0.029 0.020 0.335
H3B4 1.278 0.128 1.299 0.0142 0.489 0.126 0.035 0.063
H3B5 1.388 0.102 1.175 0.0912 0.214 0.089 0.010 0.060
H4B1 0.888 0.097 1.330 0.0334 0.764 0.089 0.013 0.121
H4B2 0.778 0.052 0.708 0.0762 0.265 0.057 0.008 0.040
H4B3 1.435 0.165 1.333 0.0224 0.850 0.127 0.030 0.086
H4B4 1.395 0.411 0.893 0.0613 0.477 0.108 0.013 0.061
H4B5 0.994 0.323 1.472 0.0483 0.677 0.382 0.020 0.077
H5B1 0.897 0.060 1.247 0.0251 0.647 0.066 0.009 0.041
H5B2 1.124 0.166 1.165 0.0837 0.100 0.120 0.011 0.082
H5B3 1.235 0.242 1.210 0.0882 0.259 0.120 0.013 0.092
H5B4 1.127 0.137 1.459 0.0633 0.918 0.080 0.020 0.110
H5B5 1.536 0.072 1.127 0.0743 0.248 0.057 0.024 0.104

Table 4

Average contents of eight mineral elements in Beiqing 9 under different fertilization conditions mg/g"

处理Treatment Ca Fe Mg Al Na Zn Cu Mn
F0 0.119 0.060 0.145 0.015 0.591 0.061 0.002 0.060
F1(N+P) 1.084 0.083 1.130 0.014 0.388 0.058 0.013 0.068
F2(N+P) 1.087 0.063 1.040 0.056 0.048 0.052 0.011 0.050
F3(N+P) 0.962 0.071 1.082 0.059 0.087 0.050 0.012 0.050
F4(N+P) 0.922 0.067 0.870 0.014 0.851 0.101 0.021 0.084
F1N 0.983 0.132 0.967 0.084 0.664 0.185 0.018 0.068
F2N 0.917 0.148 1.136 0.073 0.930 0.093 0.101 0.054
F3N 0.865 0.782 0.862 0.028 0.185 0.080 0.019 0.066
F4N 0.815 0.312 0.548 0.052 0.111 0.052 0.010 0.048
F1P 0.991 0.514 0.293 0.084 0.378 0.061 0.015 0.056
F2P 1.028 0.473 0.299 0.072 0.434 0.053 0.012 0.045
F3P 1.006 0.404 0.364 0.102 0.570 0.060 0.014 0.049
F4P 0.988 0.421 0.308 0.125 0.199 0.052 0.014 0.082

Fig.2

The contents of SOD, chlorophyll and cytochrome oxidase of Beiqing 9 under different sowing methods and fertilization conditions"

Table 5

Correlation analysis between mineral elements and chlorophyll of highland barley (n=47)"

元素Element Fe Mg Al Na Zn Cu Mn 叶绿素Chlorophyll
Fe 1.000 0.749** 0.678** 0.336* 0.459** 0.392** 0.555** -0.001
Mg 0.749** 1.000 0.680** 0.444** 0.591** 0.230 0.682** 0.057
Al 0.678** 0.680** 1.000 0.196 0.310* 0.426** 0.610** 0.020
Na 0.336* 0.444** 0.196 1.000 0.188 0.256 0.091 -0.095
Zn 0.459** 0.591** 0.310* 0.188 1.000 0.145 0.068 -0.167
Cu 0.392** 0.230 0.426** 0.256 0.145 1.000 0.066 -0.100
Mn 0.555** 0.682** 0.610** 0.091 0.068 0.066 1.000 0.327*
叶绿素Chlorophyll -0.001 0.057 0.020 -0.095 -0.167 -0.100 0.327* 1.000
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