Crops ›› 2017, Vol. 33 ›› Issue (2): 157-162.doi: 10.16035/j.issn.1001-7283.2017.02.028

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Isolation and Identification of Antagonistic Bacteria of Fusarium Head Blight of Winter Wheat in Hebei Province

Zhang Xuejiao,Shi Jingjing,Chang Na,Zhang Yaqin,Qi Yongzhi,Zhen Wenchao,Yin Baozhong   

  1. College of Plant Protection, Agricultural University of Hebei,Baoding 071001,Hebei,China
  • Received:2016-11-30 Revised:2017-02-17 Online:2017-04-15 Published:2018-08-26
  • Contact: Baozhong Yin

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Abstract:

115 soil samples were collected from 15 counties in Hebei province during the wheat growing season from 2015 to 2016. The soil samples were separated by dilution method, then 218 strains of bacteria were obtained. 27 strains with the average inhibition rate of 44.09% on Fusarium were obtained after the filteration of the 218 strains by plate confrontation method. Among them, XJ-16 and LY-2 had good antagonistic effect, the inhibition rates were 73.76% and 73.73%, respectively. The morphological, physiological, biochemical and molecular identification of strain XJ-16 and LY-2 were carried out. The results showed that both two strains were Bacillus amyloliquefaciens.

Key words: Hebei province, Wheat, Fusarium head blight, Antagonistic bacteria, Separate, Identification

Table 1

Antimicrobial rate of antagonistic microorganisms %"

菌株编号Strain code 抑菌率Antimicrobial rate 菌株编号Strain code 抑菌率Antimicrobial rate 菌株编号Strain code 抑菌率Antimicrobial rate
XJ-16 73.76±1.35a WD-15 49.83±2.48bc ZQ-23 33.53±3.30d
LY-2 73.73±1.45a XJ-18 49.03±1.48bc WD-17 32.73±2.79d
ZQ-14 63.07±1.58b XJ-17 47.43±2.32bc XJ-13 29.56±2.11d
XJ-3 61.23±2.77b WD-8 47.40±3.36bc QX-1 29.03±1.32d
XJ-7 59.70±2.81b QX-16 46.90±4.94bc WD-24 26.83±2.90d
LY-9 58.73±1.55b XJ-19 45.20±1.99bc LY-10 26.73±3.40d
XJ-20 55.67±2.82b LY-12 43.10±2.74c LY-6 25.93±2.85d
QX-6 52.33±3.04b ZQ-8 37.83±1.55d QX-5 20.97±4.05e
LY-11 50.43±2.15bc WD-20 35.93±2.73d ZQ-19 20.93±2.34e

Fig.1

The antibacterial effects of XJ-16 and LY-2"

Fig.2

Colony morphology of XJ-16 and LY-2"

Fig.3

Bacterial morphology of XJ-16 and LY-2"

Table 2

Physiological and biochemical characteristics of strains XJ-16 and LY-2"

指标Index XJ-16 LY-2 指标Index XJ-16 LY-2
运动性 - - 水解 明胶 + +
接触酶 + + 淀粉 + +
氧化酶 - - 酪素 + +
厌氧生长 + + 利用 柠檬酸盐 - -
甲基红试验反应 - - 丙二酸盐 - -
乙酰甲基甲醇试验 + + 酪氨酸水解 - -
产生吲哚 - - 苯丙氨酸脱氨酶 - -
发酵 D-葡萄糖 + + 硝酸盐还原 + +
L-阿拉伯糖 + + 水解纤维素 + +
D-木糖 + + 生长 pH5.7营养肉汤 + +
D-甘露醇 + + 葡萄糖产气 - -
KOH溶液简易鉴定 - - 革兰氏染色 + +

Fig.4

Phylogenetic tree of the strains of LY-2 and XJ-16 based on gyrB sequence"

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