Crops ›› 2017, Vol. 33 ›› Issue (5): 43-48.doi: 10.16035/j.issn.1001-7283.2017.05.008

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Identification of Cold Tolerance in Seven Wild Sugarcane and Three Offsprings at Seedling Stage

Cao Zhequn,Xiao Furong,Chen Shuying,He Lilian,Li Fusheng   

  1. College of Agronomy and Biotechnology,Yunnan Agricultural University,Kunming 650201,Yunnan,China
  • Received:2017-07-02 Revised:2017-08-03 Online:2017-10-15 Published:2018-08-26
  • Contact: Lilian He,Fusheng Li

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Abstract:

Using seven wild sugarcane (Erianthus fulvus Ness.) and its three offsprings with sugarcane hybrid materials (yunnan sugarcane varieties/lines) as the materials to determinate the physiological and biochemical indexes related to cold tolerance at the seedling stage in low temperature stress (3℃). The results showed: (1) Through the pole sorting method to calculate the electrical conductivity, the content of soluble sugar, MDA, proline, soluble protein, chlorophyll, and superoxide dismutase (SOD) activity, the weight coefficient are 0.109, 0.194, 0.148, 0.133, 0.108, 0.183,and 0.124,respectively.The weight coefficients of soluble sugar content and chlorophyll content were the highest, which indicated the two physiological indexes had stronger effects on cold-tolerance. (2) Under the stress of low temperature, the cold tolerance of 10 materials strength in the order: Zhemao99-3 > Zhemao99-2 > Zhemao Ⅱ91-2 > Zhemao99-4 > Zhemao99-1 > Zhemao90-29 > Zhemao Ⅰ91-8 > Dianzhe04-14 > Dianzhe01-58 > Dianzhe02-39. In conclusion, the cold tolerance of wild sugarcane were better than domestic sugarcane cultivars.

Key words: Wild sugarcane, Sugarcane, Cold tolerance, Seedling stage, Pole-sorting method

Table 1

Effects of low temperature on conductivity and soluble sugar content"

材料
Material		 电导率Conductivity(μS/cm) 电导率相对值(%)
Relative value of conductivity		 可溶性糖含量Soluble sugar content(mg/g) 可溶性糖含量相对值(%)
Relative value of soluble sugar content
常温
Normal temperature		 低温
Low temperature		 常温
Normal temperature		 低温
Low temperature
ZM1 0.12±0.01cdBC 0.17±0.01abABC 138.1* 21.82±4.651abA 23.15±0.93abcABC 106.1
ZM2 0.14±0.03bcAB 0.15±0.00bcdBCD 105.0 20.44±4.85abA 21.76±1.11bcABC 106.4
ZM3 0.16±0.03abA 0.17±0.01abABC 101.7 21.22±6.24abA 28.42±1.45abAB 133.9
ZM4 0.18±0.01aA 0.20±0.02aA 108.5 19.82±2.60abA 29.95±1.16aA 151.1**
ZM5 0.17±0.01aA 0.19±0.01aAB 108.9 18.29±3.67abA 19.56±3.10cBCD 106.9
ZM6 0.11±0.01cdeBC 0.14±0.03cdCDE 119.8 14.57±0.92bA 23.15±1.63abcABC 159.0*
ZM7 0.11±0.02cdeBC 0.15±0.00bcBCD 134.9* 23.30±2.16aA 23.67±5.28abcABC 101.6
DZ1 0.08±0.01eC 0.12±0.01deDE 145.9* 18.20±3.14abA 12.00±0.78dD 67.6
DZ2 0.09±0.02deBC 0.14±0.03cdCDE 139.8* 21.93±6.03abA 20.90±1.44cABCD 95.3
DZ3 0.09±0.01eC 0.10±0.00eE 116.3 16.51±0.44abA 18.29±0.43cdCD 110.8

Table 2

Effects of low temperature treatment on MDA content and chlorophyll content"

材料
Material		 MDA含量MDA content (nmol/g) MDA含量相对值(%)
Relative value of MDA content		 叶绿素含量Chlorophyll content (mg/g) 叶绿素含量相对值(%)
Relative value of chlorophyll content
常温
Normal temperature		 低温
Low temperature		 常温
Normal temperature		 低温
Low temperature
ZM1 2.92±0.78bcABC 3.76±0.73abAB 128.7 2.91±0.61abAB 2.72±0.07bAB 93.3
ZM2 2.89±0.42bcABC 3.29±0.12bcdABCD 113.8 3.30±0.19aA 3.23±0.04aA 97.9
ZM3 3.70±0.43aA 3.84±0.35abAB 103.8 2.43±0.50bcAB 2.25±0.19cB 92.4
ZM4 3.33±0.38abAB 3.61±0.04abcABC 108.3 2.36±0.39bcB 1.72±0.21dC 73.1*
ZM5 2.59±0.17bcdBCD 4.40±0.80aA 170.2** 2.44±0.38bcAB 2.42±0.22bcB 99.2
ZM6 2.09±0.42defCDE 2.42±0.17efCD 115.7 2.92±0.21abAB 2.47±0.31bcB 84.7
ZM7 2.23±0.33cdeCDE 3.52±0.08abcABC 157.6** 2.36±0.17bcB 2.35±0.48bcB 99.5
DZ1 1.66±0.54efDE 2.12±0.60fD 128.1 2.32±0.11bcB 1.33±0.10deCD 57.2**
DZ2 1.42±0.17fE 2.35±0.461fCD 165.9** 2.14±0.15cB 1.01±0.10eD 47.2**
DZ3 1.93±0.23defCDE 2.75±0.70defBCD 142.2 2.24±0.48bcB 1.68±0.14dC 74.9*

Table 3

Effects of low temperature on proline content and soluble protein content"

材料
Material		 Pro含量Pro content (μg/g) Pro含量相对值(%)
Relative value of
pro content		 可溶性蛋白含量Soluble protein content (mg/g) 可溶性蛋白含量相对值(%)
Relative value of soluble protein content
常温
Normal temperature		 低温
Low temperature		 常温
Normal temperature		 低温
Low temperature
ZM1 38.54±4.53cBC 63.28±7.37bAB 164.2** 13.07±2.12bcB 14.65±2.79cdBCD 112.1
ZM2 43.25±6.38bcABC 82.15±5.11aA 190.0** 20.56±4.18aA 27.89±2.46aA 135.2
ZM3 56.45±11.36aA 81.97±7.95aA 145.2** 14.14±3.77bcAB 16.74±0.44bcBC 118.3
ZM4 52.43±4.02abAB 65.12±12.63bAB 124.2 11.51±0.69cB 11.85±0.21dD 103.0
ZM5 54.05±9.87abAB 61.71±10.32bAB 114.2 12.97±0.64bcB 18.56±0.88bB 143.1*
ZM6 35.35±3.48cC 46.11±5.29cB 130.4 12.80±0.73bcB 12.91±2.96dCD 100.9
ZM7 41.40±3.23bcABC 59.22±6.00bcB 143.0* 12.61±0.59bcB 13.35±0.50dCD 105.9
DZ1 46.48±5.88abcABC 61.02±2.68bcAB 131.3* 13.33±1.07bcAB 13.50±0.19dCD 101.2
DZ2 42.32±0.89bcABC 66.46±1.80bAB 157.0** 11.78±3.10cB 12.85±0.11dCD 109.1
DZ3 54.83±2.91aA 65.86±1.53bAB 120.1 17.70±5.96abAB 17.87±1.78bB 100.9

Table 4

Effects of low temperature on the activity of SOD"

材料
Material		 常温(U/g)
Normal temperature		 低温(U/g)
Low temperature		 相对值(%)
Relative value
ZM1 209.63±34.33cdBCD 163.04±23.78cdC 77.8*
ZM2 220.29±30.85bcB 173.90±29.10cdBC 78.9*
ZM3 232.64±3.72bcBC 229.66±1.95abAB 98.7
ZM4 248.77±29.91bcB 194.86±16.53bcABC 78.3*
ZM5 331.27±14.98aA 182.02±15.20cdBC 54.9**
ZM6 211.37±21.76cdBCD 194.86±21.80bcABC 92.2
ZM7 165.87±31.48eD 142.34±41.57dC 85.8
DZ1 207.07±31.39cdBCD 191.46±5.00bcABC 92.5
DZ2 180.32±3.98deCD 132.85±20.59cdC 73.7*
DZ3 269.44±14.00bB 244.62±18.83aA 90.8

Table 5

Evaluation matrix"

材料
Material		 电导率
Conductivity		 可溶性糖含量
Soluble sugar content		 MDA含量
MDA content		 脯氨酸含量
Pro content		 可溶性蛋白含量
Soluble protein content		 叶绿素含量
Chlorophyll content		 SOD活性
SOD activity
ZM1 1.88 4.13 2.33 5.41 3.11 4.30 3.61
ZM2 5.62 5.25 5.07 5.84 3.13 6.00 3.74
ZM3 6.00 6.00 3.07 5.32 4.63 3.05 6.00
ZM4 5.23 5.66 1.25 3.47 5.57 1.66 3.67
ZM5 5.18 1.00 6.00 5.97 3.15 1.00 1.00
ZM6 3.95 5.10 1.00 4.58 6.00 2.07 5.25
ZM7 2.24 1.95 1.59 6.00 2.86 2.90 4.53
DZ1 1.00 4.18 1.04 1.96 1.00 2.13 5.28
DZ2 1.69 1.33 1.98 1.00 2.52 3.83 3.14
DZ3 4.34 3.11 1.00 3.65 3.36 1.39 5.09

Table 6

Evaluation results of cold resistance of sugarcane"

材料
Material		 电导率
Conductivity		 可溶性糖含量
Soluble sugar content		 MDA含量
MDA content		 脯氨酸含量
Pro content		 可溶性蛋白含量
Soluble protein content		 叶绿素含量
Chlorophyll content		 SOD活性
SOD activity		 综合评价值
Comprehensive evaluation value		 排名
Ranking
ZM1 0.21 0.60 0.61 0.57 0.25 0.99 0.45 3.68 5
ZM2 0.61 0.61 0.78 0.80 0.55 1.07 0.47 4.88 2
ZM3 0.66 0.90 0.89 0.40 0.33 0.97 0.75 4.90 1
ZM4 0.57 1.08 0.84 0.22 0.14 0.63 0.46 3.94 4
ZM5 0.57 0.61 0.15 0.13 0.65 1.09 0.12 3.32 6
ZM6 0.43 1.17 0.76 0.27 0.11 0.84 0.65 4.23 3
ZM7 0.24 0.56 0.29 0.38 0.17 1.10 0.56 3.31 7
DZ1 0.11 0.19 0.62 0.28 0.11 0.36 0.66 2.33 9
DZ2 0.18 0.49 0.20 0.51 0.21 0.18 0.39 2.17 10
DZ3 0.47 065 0.46 0.18 0.11 0.67 0.63 3.18 8
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