Crops ›› 2016, Vol. 32 ›› Issue (2): 21-26.doi: 10.16035/j.issn.1001-7283.2016.02.004

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Identification of Cold Tolerance at Germination Stage in Maize Inbred Lines

Zhang Xiaocong1,Zhou Yu1,Zhang Lin1,Di Hong1,Weng Jianfeng2,Li Xinhai2,Wang Zhenhua1   

  1. 1 College of Agriculture,Northeast Agricultural University,Harbin 150030,Heilongjiang,China
    2 Institute of Crop Science,Chinese Academy of Agricultural Sciences/National Engineering Laboratory for Crop Molecular Breeding,Beijing 100081,China
  • Received:2016-01-27 Revised:2016-02-23 Online:2016-04-15 Published:2018-08-26
  • Contact: Xinhai Li,Zhenhua Wang

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Abstract:

Cold stress is one of the major meteorological disasters in the spring maize-growing area in northern China. Cold tolerance of eight inbred lines widely used in northeastern China was evaluated through a laboratory germination experiment under low temperature stress. Germination potential, germination rate, average time to germination, germination index, dry weight of seedlings, and vigor index at normal temperature (25℃) and low temperature (10℃) were measured, respectively, and the ratios (relative values) of trait values at low temperature to those at normal temperature were used as indicators for cold tolerance. The cluster analysis revealed that the eight inbred lines could be classified into three groups: PH4CV, Ji 419, and Ji 495 belonged to high cold tolerance, H21 belonged to intermediate cold tolerance, and W9706, Huangzaosi, Danhuang 02, and Si 279 belonged to low cold tolerance. The correlation analysis showed that the relative values of germination rate, germination index, dry weight of seedlings, and vigor index were highly significantly correlated with each other, and they were suitable indicators for cold tolerance. This experiment provided guidance to identify and screen maize germplasm with cold tolerance at the germination stage on a large scale.

Key words: Maize, Germination, Cold tolerance, Cluster analysis, Correlation analysis

Table 1

Changes of germination potential and germination rate in maize inbred lines under low temperature stress %"

自交系
Inbred line		 发芽势Germination potential 发芽率Germination rate
25℃ 10℃ 相对值Relative value 25℃ 10℃ 相对值Relative value
PH4CV 98.89a 32.22ab 0.33 100.00a 96.67a 0.97
吉495 Ji 495 96.67a 26.67b 0.28 97.78a 95.56a 0.98
吉419 Ji 419 80.00c 33.33a 0.42 90.00c 86.67b 0.96
H21 95.56ab 28.89b 0.30 97.78a 72.22c 0.74
四279 Si 279 91.11b 36.67a 0.40 96.67ab 62.22d 0.64
丹黄02Danhuang 02 78.89c 12.22c 0.15 90.00c 61.11d 0.68
W9706 75.56c 13.33c 0.18 93.33bc 47.78e 0.51
黄早四Huangzaosi 76.67c 14.44c 0.19 94.44bc 56.67d 0.60

Table 2

Changes of average time to germination and germination index in maize inbred lines under low temperature stress"

自交系
Inbred line		 平均发芽时间（d）
Average time to germination		 发芽指数
Germination index
25℃ 10℃ 相对值Relative value 25℃ 10℃ 相对值Relative value
PH4CV 3.09c 18.05c 5.84 9.78ab 1.76a 0.18
吉495 Ji 495 3.37bc 20.15c 5.98 8.24cd 1.40ab 0.17
吉419 Ji 419 3.65ab 21.24bc 5.82 7.35de 1.25bc 0.17
H21 3.49b 21.22bc 6.08 10.47a 1.43ab 0.14
四279 Si 279 3.62ab 23.24b 6.42 8.93bc 1.16bc 0.13
丹黄02Danhuang 02 3.90a 26.71a 6.85 7.41de 0.89cd 0.12
W9706 3.81a 23.47ab 6.16 6.53e 0.74d 0.11
黄早四Huangzaosi 3.95a 23.98ab 6.07 7.37de 0.88cd 0.12

Table 3

Changes of dry weight of seedlings and vigor index in maize inbred lines under low temperature stress g"

自交系
Inbred line		 幼苗干重Dry weight of seedlings 活力指数Vigor index
25℃ 10℃ 相对值Relative value 25℃ 10℃ 相对值Relative value
PH4CV 0.12ab 0.05a 0.38 1.19a 0.08a 0.07
吉495 Ji 495 0.13a 0.04ab 0.32 1.11a 0.06ab 0.05
吉419 Ji 419 0.12ab 0.04ab 0.35 0.91ab 0.05ab 0.06
H21 0.10c 0.02c 0.22 1.09a 0.03b 0.03
四279 Si 279 0.08d 0.01cd 0.18 0.76bc 0.02b 0.02
丹黄02Danhuang 02 0.09cd 0.01cd 0.16 0.70bc 0.01b 0.02
W9706 0.09cd 0.02c 0.17 0.61c 0.01b 0.02
黄早四Huangzaosi 0.08d 0.02c 0.18 0.61c 0.01b 0.02

Fig.1

Cluster analysis of eight maize inbred lines"

Table 4

The mean relative values of traits in three groups of maize inbred lines with different cold tolerance"

类群
Group		 相对发芽势
Relative germination potential		 相对发芽率
Relative germination
rate		 相对平均发芽时间
Relative average time
to germination		 相对发芽指数
Relative germination
index		 相对幼苗干重
Relative dry weight
of seedlings		 相对活力指数
Relative vigor
index
I 0.34 0.97 5.88 0.17 0.35 0.06
II 0.30 0.74 6.08 0.14 0.22 0.03
III 0.23 0.61 6.38 0.12 0.17 0.02

Table 5

Correlation coefficients among relative values of traits in maize inbred lines"

项目Item 相对发芽势Relative germination potential 相对发芽率Relative germination rate 相对发芽指数Relative germination index 相对平均发芽时间Relative average time to germination 相对幼苗干重Relative dry weight of seedlings
相对发芽率 0.56
相对发芽指数 0.64 0.98**
相对平均发芽时间 -0.48 -0.55 -0.66
相对幼苗干重 0.59 0.94** 0.98** -0.75
相对活力指数 0.56 0.93** 0.96** -0.71 0.99**
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