Crops ›› 2021, Vol. 37 ›› Issue (2): 160-164.doi: 10.16035/j.issn.1001-7283.2021.02.023

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Effects of Three Growth Regulators on the Growth of Potato Test-Tube Seedlings

Qiu Tian1(), Niu Lili2, Zhu Jiang2, Cai Fuge2, Wang Qingwei2   

  1. 1College of Agriculture, Anshun University, Anshun 561000, Guizhou, China
    2Anshun Academy of Agricultural Sciences, Anshun 561000, Guizhou, China
  • Received:2020-06-07 Revised:2020-07-06 Online:2021-04-15 Published:2021-04-16

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Abstract:

To improve the quality of test-tube seedlings and optimize the virus-free subculture of potato, Yanshu No. 4 virus-free seedlings were used as the test material, and 6-BA (0.5mg/L, 1.0mg/L, 1.5mg/L), salicylic acid (0.2mg/L, 0.4mg/L, 0.6mg/L) and brassinolide (0.01mg/L, 0.10mg/L, 1.00mg/L) was selected as the treatments, while, no growth regulator was added in control. The growth morphological characteristics, chlorophyll content, root vitality, survival rate and tuber formation characteristics of three growth regulators on potato test-tube seedlings were studied. The results showed the three growth regulators could increase plant height, stem diameter, fresh weight, average root length, root strip number and leaf number. In addition, the chlorophyll content, root vitality, survival rate and tuber formation characteristics after transplanting were all better under the treatment of 6-BA, salicylic acid and brassinolide are 1.0mg/L, 0.4mg/L and 1.0mg/L, respectively. After transplanting, the survival rate was the highest, and the tuber formation characteristics were better under the treatment of salicylic acid (0.4mg/L), which is the most suitable medium for strong seedlings.

Key words: Potato, Growth regulator, Test-tube seedling

Table 1

Type and concentration of growth regulators in each treatment"

编号
Number		 生长调节剂
Growth regulator		 浓度
Concentration (mg/L)		 pH
CK - - 5.8
A1 6-BA 0.5 5.8
A2 1.0 5.8
A3 1.5 5.8
B1 SA 0.2 5.8
B2 0.4 5.8
B3 0.6 5.8
C1 BR 0.01 5.8
C2 0.10 5.8
C3 1.00 5.8

Table 2

Effects of three growth regulators on the growth of potato test-tube seedlings"

处理
Treatment		 株高
Plant height (mm)		 茎粗
Stem diameter (mm)		 鲜重
Fresh weight (g)		 平均根长
Average root length (mm)		 根条数
Number of roots		 叶片数
Number of leaves
CK 56.73±1.74e 0.75±0.02def 0.09±0.002de 26.87±1.48f 4.43±0.37f 6.45±0.20c
A1 63.81±1.94d 0.78±0.01cd 0.11±0.002c 34.94±2.09de 5.58±0.15e 8.11±0.20b
A2 75.05±1.93b 0.83±0.02ab 0.14±0.006b 49.43±2.97b 8.19±0.49b 9.24±0.02a
A3 54.73±1.20ef 0.77±0.02de 0.09±0.004de 28.08±1.36d 6.83±0.08d 6.45±0.39c
B1 67.96±1.85c 0.77±0.03de 0.12±0.003c 38.60±2.83cd 7.04±0.09d 6.67±0.25c
B2 85.74±2.63a 0.85±0.03a 0.16±0.009a 59.71±3.64a 10.97±0.52a 8.61±0.31b
B3 52.36±1.66f 0.74±0.02ef 0.09±0.004e 41.66±2.81c 7.60±0.22c 5.69±0.43d
C1 53.20±1.80f 0.73±0.02f 0.09±0.005e 32.95±2.32e 5.58±0.34e 5.71±0.54d
C2 57.44±2.34e 0.77±0.02de 0.10±0.006d 41.20±2.42c 6.66±0.26d 6.88±0.61c
C3 64.81±2.29cd 0.81±0.02bc 0.12±0.006c 61.46±3.06a 8.47±0.17b 8.06±0.05b

Fig.1

The effects of three growth regulators on the chlorophyll content of potato test-tube seedlings Different lowercase letters indicate difference at 0.05 level, the same below"

Fig.2

The effects of three growth regulators on the root vitality of potato test-tube seedlings"

Table 3

Effects of three growth regulators on the survival rate of potato test-tube seedlings and tuber formation characteristics after transplanting"

处理
Treatment		 平均成活率
Average survival rate (%)		 单株结薯数
Number of tubers per plant		 单株结薯重
Potato weight per plant (g)		 大薯率
Percentage of big-tuber (%)
CK 37.01±1.65f 0.98±0.04c 0.49±0.02f 0.00±0.00e
A1 41.22±1.81ef 0.98±0.06c 0.51±0.03f 9.58±1.05ab
A2 73.59±5.62bc 1.22±0.04b 0.73±0.05e 8.40±0.67bc
A3 52.55±4.72d 1.03±0.05c 0.92±0.05b 5.60±0.53d
B1 67.69±4.08c 1.04±0.07c 0.85±0.04c 6.14±0.64d
B2 85.77±2.90a 1.32±0.04a 1.09±0.04a 6.04±0.05d
B3 30.43±4.27g 0.86±0.07d 0.42±0.02g 10.31±1.15a
C1 46.24±2.27e 1.06±0.06c 0.52±0.04f 10.26±1.33a
C2 55.87±3.57d 1.16±0.05b 0.75±0.05de 7.77±0.33c
C3 77.03±2.36b 1.38±0.02a 0.79±0.02cd 8.76±0.12bc
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