Crops ›› 2021, Vol. 37 ›› Issue (4): 144-151.doi: 10.16035/j.issn.1001-7283.2021.04.022

Previous Articles     Next Articles

Effects of Iron, Cadmium and Their Interaction on the Primary Reaction of Photosynthesis in Rice

Yang Lei(), Jin Yandi, Liu Houjun()   

  1. College of Land and Environment, Shenyang Agricultural University, Shenyang 110866, Liaoning, China
  • Received:2020-08-14 Revised:2020-11-13 Online:2021-08-15 Published:2021-08-13
  • Contact: Liu Houjun E-mail:1026542765@qq.com;liuhoujun_0@syau.edu.cn

RichHTML

3

PDF (PC)

229

Abstract:

Iron (Fe) plays an important role in plant photosynthesis, while cadmium (Cd) causes various interferes in photosynthesis. Chlorophyll fluorescence reflects photosynthetic efficiency and electron transfer characteristics. This experiment aimed to investigate the effects of Fe, Cd and their interactions on photosynthetic efficiency and chlorophyll fluorescence in rice. The pot experiment was designed to investigate photosynthetic indexes, chlorophyll fluorescence indexes, and OJIP curve under different Fe and Cd supplements in soil. The rate of Fe addition was 0 (Fe0), 1.0 (Fe1) and 2.0g/kg (Fe2), while Cd was 0 (Cd0) and 2.0mg/kg (Cd2). The results showed that Fe1 treatment increased photosynthetic rate (Pn), stomatal conductance (Gs), intercellular CO2 concentration (Ci), and fluorescence intensity at the early stage, while Fe2 and Cd2 decreased these indexes. At the late stages (after the booting stage), Fe1 and Fe2 were beneficial for rice to keep high Pn, Gs, Ci and chlorophyll fluorescence intensity but Cd2 still decreased these indexes. Fe or Cd increased the maximum photochemical efficiency (Fv/Fm) at the early stages, while at the late stages, Cd alone or Cd and high Fe together significantly decreased Fv/Fm. Fe1 significantly promoted the photosynthesis of rice and alleviated the interference of Cd on photosynthesis, while Fe2 significantly inhibited photosynthesis and reduced dry matter accumulation in rice. Cd2 decreased photosynthesis and disturbed the physiological function of Fe during photosynthesis. Therefore, proper application of Fe fertilizer can promote the photosynthesis of rice and reduce Cd toxicity.

Key words: Iron, Cadmium, Rice, Primary reaction of photosynthesis, Chlorophyll fluorescence

Fig.1

Dry weight of grains and shoots of rice at harvest under different Fe, Cd treatments Different lowercase letters indicate significant difference among Fe treatments at the same Cd level (P<0.05)"

Fig.2

Pn, Ci, SPAD value and Gs in leaves of rice at different growth stages"

Table 1

Analysis of variance on Pn, Ci, SPAD value and Gs in leave of rice at different growth stages"

指标
Index		 处理
Treatment		 分蘖期
Tillering stage		 拔节期
Jointing stage		 孕穗期
Booting stage		 扬花期
Flowering stage		 蜡熟期
Ripening stage
Pn Cd * ** ns * **
Fe ** ** ns * **
Cd×Fe ns ns * ns *
Ci Cd ns ** ns ns ns
Fe ** ** * ** **
Cd×Fe ns ** ns ** **
SPAD值SPAD value Cd ns ** ns ns ns
Fe ** ** ** ** **
Cd×Fe ns ns ns ns ns
Gs Cd ** ** ns ns **
Fe ** ** ns ** **
Cd×Fe ns ** ns ns ns

Fig.3

Fv/Fm, PI, Fo and Fm in leaves of rice at different growth stages"

Table 2

Analysis of variance on Fv/Fm, PI, Fo, and Fm in leaves of rice at different growth stages"

指标
Index		 处理
Treatment		 分蘖期
Tillering		 拔节期
Jointing		 孕穗期
Booting		 扬花期
Flowering		 蜡熟期
Ripening
Cd ns ns * ns ns
Fv/Fm Fe * ns ns ns ns
Cd×Fe ** ns ns ns ns
PI Cd ns ns ns ns ns
Fe ** ** ns ** **
Cd×Fe ** ns ns ns ns
Fo Cd ns ns ns ns ns
Fe ** ** ns ns **
Cd×Fe ns ns ns ** ns
Fm Cd ns ns ** ns ns
Fe * * * ** **
Cd×Fe * ns ns ns *

Fig.4

Kinetic curve of chlorophyll fluorescence intensity of leaves of rice at different growth stages O, J, I, P indicate fluorescent signal at 10-15μs, 2ms, 30ms and 0.3-2000ms after illumination, the same below"

Fig.5

Kinetic curve of relative chlorophyll fluorescence intensity of leaves of rice at different growth stages"

[1] Yadavalli V, Neelam S, Rao A, et al. Differential degradation of photosystem I subunits under iron deficiency in rice. Journal of Plant Physiology, 2012,169(8):753-759.
[2] Adamski J M, Peters J A, Danieloski R, et al. Excess iron-induced changes in the photosynthetic characteristic of sweet potato. Journal of Plant Physiology, 2011,168(17):2056-2062.
[3] Hänsch R, Mendel R R. Physiological functions of mineral micronutrients (Cu,Zn,Mn,Fe,Ni,Mo,B,Cl). Current Opinion in Plant Biology, 2009,12(3):259-266.
[4] Vigani G, Zocchi G, Bashir K, et al. Signals from chloroplasts and mitochondria for iron homeostasis regulation. Trends in Plant Science, 2013,18(6):305-311.
[5] Daud M K, He Q L, Lei M, et al. Ultrastructural,metabolic and proteomic changes in leaves of upland cotton in response to cadmium stress. Chemosphere, 2015,120:309-320.
[6] Zhou Y, Diao M, Cui J X, et al. Exogenous GSH protects tomatoes against salt stress by modulating photosystem II efficiency,absorbed light allocation and H2O2-scavenging system in chloroplasts. Journal of Integrative Agriculture, 2018,17(10):2257-2272.
[7] Liu H J, Yang L, Li N, et al. Cadmium toxicity reduction in rice (Oryza sativa L.) through iron addition during primary reaction of photosynthesis. Ecotoxicology and Environmental Safety, 2020,200:1-9.
[8] Srivastava R K, Pandey P, Rajpoot R, et al. Cadmium and lead interactive effects on oxidative stress and antioxidative responses in rice seedlings. Protoplasma, 2014,251:1047-1065.
[9] 聂艳秋, 李玉秀, 刘安辉, 等. 铁肥形态及施用方式对印度芥菜镉积累的影响. 环境科学与技术, 2012,35(12):51-55.
[10] 邵国胜, 陈铭学, 王丹英, 等. 稻米镉积累的铁肥调控. 中国科学, 2008,38(2):180-187.
[11] Liu H J, Zhang C X, Wang J M, et al. Influence and interaction of iron and cadmium on photosynthesis and antioxidative enzymes in two rice cultivars. Chemosphere, 2017,171:240-247.
[12] Siedlecka A, Krupa Z. Interaction between cadmium and iron and its effects on photosynthetic capacity of primary leaves of Phaseolus vulgaris. Plant Physiology and Biochemistry, 1996,34(6):833-841.
[13] Qureshi M I, Amici D’ G M, Fagioni M, et al. Iron stabilizes thylakoid protein-pigment complexes in Indian mustard during Cd-phytoremediation as revealed by BN-SDS-PAGE and ESI-MS/MS. Journal of Plant Physiology, 2010,167(10):761-770.
[14] Sárvári É, Solti Á, Basa B, et al. Impact of moderate Fe excess under Cd stress on the photosynthetic performance of poplar (Populus jacquemontiana var. glauca cv. Kopeczkii). Plant Physiology and Biochemistry, 2011,49(5):499-505.
[15] Strasser R J, Srivastava A, Govindjee. Polyphasic chlorophyll a fluorescence transient in plants and cyanobacteria. Photochemistry and Photobiology, 1995,61(1):32-42.
[16] 黄光福, 唐巧玲, 唐云鹏, 等. 叶绿素荧光分析技术在水稻研究中的应用. 作物研究, 2013,27(2):174-179.
[17] 李鹏民, 高辉远, Strasser R J. 快速叶绿素荧光诱导动力学分析在光合作用研究中的应用. 植物生理与分子生物学学报, 2005,31(6):559-566.
[18] Pereira E G, Oliva M A, Rosado-Souza L, et al. Iron excess affects rice photosynthesis through stomatal and non-stomatal limitations. Plant Science, 2013,201-202:81-92.
[19] Monteiro M S, Santos C, Soares A, et al. Assessment of biomarkers of cadmium stress in lettuce. Ecotoxicology and Environmental Safety, 2009,72(3):811-818.
[20] 周坤, 刘俊徐, 卫红, 等. 铁对番茄镉积累及其化学形态的影响. 园艺学报, 2013,40(11):2269-2279.
[21] 贺国强, 刘茜, 郭振楠, 等. 镉胁迫对烤烟叶片光合和叶绿素荧光特性的影响. 华北农学报, 2016,31(S1):388-393.
[22] 赵婧, 邱强, 张鸣浩, 等. 大豆在不同水铁平下生理特性与品种耐性的关系. 核农学报, 2016,30(11):2239-2247.
[23] Briat J F, Curie C, Gaymard F. Iron utilization and metabolism in plants. Current Opinion in Plant Biology, 2007,10(3):276-282.
[24] Nenova V R. Growth and photosynthesis of pea plants under different iron supply. Acta Physiologiae Plantarum, 2009,31(2):385-91.
[25] Strasser R J. Donor side capacity of photosystem II probed by chlorophyll a fluorescence transients. Photosynthesis Research, 1997,52(2):147-155.
[26] Siedlecka A, Baszyń Aski T. Inhibition of electron flow around photosystem I in chloroplasts of Cd‐treated maize plants is due to Cd‐induced iron deficiency. Physiologia Plantarum, 2010,87(2):199-202.
[27] Sebastian A, Prasad M N V. Iron- and manganese-assisted cadmium tolerance in Oryza sativa L.:lowering of rhizotoxicity next to functional photosynthesis. Planta, 2015,241(6):1519-1528.
[28] Sebastian A, Prasad M N V. Operative photo assimilation associated proteome modulations are critical for iron-dependent cadmium tolerance in Oryza sativa L. Protoplasma, 2015,252(5):1375-1386.
[29] Chen Z, Tang Y T, Zhou C, et al. Mechanisms of Fe biofortification and mitigation of Cd accumulation in rice (Oryza sativa L.) grown hydroponically with Fe chelate fertilization. Chemosphere, 2017,175:275-285.
[30] Solti Á, Gáspár L, Mészáros I, et al. Impact of iron supply on the kinetics of recovery of photosynthesis in Cd-stressed poplar (Populus glauca). Annals of Botany, 2008,102(5):771-782.
[31] Solti Á, Sárvári É, Tóth B, et al. Incorporation of iron into chloroplast triggers the restoration of cadmium induced inhibition of photosynthesis. Journal of Plant Physiology, 2016,202:97-106.
[32] Sharma S S, Kaul S, Metwally A, et al. Cadmium toxicity to barley (Hordeum vulgare) as affected by varying Fe nutritional status. Plant Science, 2004,166(5):1287-1295.
[33] Xu S S, Lin S Z, Lai Z X. Cadmium impairs iron homeostasis in Arabidopsis thaliana by increasing the polysaccharide contents and the iron-binding capacity of root cell walls. Plant and Soil, 2015,392(1/2):71-85.
[34] 戴前莉, 李金花, 胡建军, 等. 增施铁对镉胁迫下柳树生长及光合生理性能的改善. 南京林业大学学报(自然科学版), 2017,41(2):63-72.
[1] Pan Gaofeng, Wang Benfu, Chen Bo, Fang Zhenbing, Zhao Shasha, Tian Yonghong. Effects of Seeding Date on Yield, Growth Period and Utilization of Temperature and Sunshine of Different Types of Japonica Rice in North Central of Hubei Province [J]. Crops, 2021, 37(4): 105-111.
[2] Tong Tianyi, Cai Jianxuan, Zhang Jisheng, Li Lin, Ma Lin, He Roujing, Tang Xiangru. Effects of Fertilizer Types on Yield, Quality and Aroma of Fragrant Rice [J]. Crops, 2021, 37(4): 152-158.
[3] Wu Ke, Xie Huimin, Liu Wenqi, Mo Bingmao, Wei Guoliang, Lu Xian, Li Zhuanglin, Deng Senxia, Wei Shanqing, Liang He, Jiang Ligeng. Effects of Nitrogen, Phosphorus and Potassium Fertilizer on Rice Grain Yield and Yield Components in Double Cropping Rice Area of Southern China [J]. Crops, 2021, 37(4): 178-183.
[4] Ling Chen, Liu Hong, Yang Zhe, Huang Zhanquan, Chen Mengqiang, Rao Dehua, Xu Zhenjiang. Effects of Double-Cropping Rice Cultivation on the Expression of Quantitative Characteristics of Rice DUS Testing Example Varieties [J]. Crops, 2021, 37(4): 18-25.
[5] Zhang Jun, Deng Dasun, Liu Jianjun, Zhou Wenxi, Huang Qianru, Zhang Weijian. Mechanization Cultivation Model of Ratoon Rice with Straw Incorporation in Double Rice Cropping Region in South of Yangtze River Valley [J]. Crops, 2021, 37(4): 212-216.
[6] Zhang Quanfang, Jiang Mingsong, Chen Feng, Zhu Wenyin, Zhou Xuebiao, Yang Lianqun, Xu Jiandi. Analysis of Genetic Diversity of Rice Varieties (Lines) in Shandong Province [J]. Crops, 2021, 37(4): 26-31.
[7] Gao Qing, Zhang Yaling, Zhou Yili, Yu Lianpeng, Nie Qiang, Jin Xuehui. Identification of Major Resistance Genes and Resistance Evaluation to Rice Blast in Japonica Rice Varieties in Heilongjiang Province [J]. Crops, 2021, 37(4): 59-66.
[8] Wang Guojiao, Song Peng, Yang Zhenzhong, Zhang Wenzhong. Effects of Straw Returning on Photosynthetic Matter Production Characteristics, Quality of Rice and Soil Nutrients [J]. Crops, 2021, 37(4): 67-72.
[9] Xue Jingfang, Cai Yongsheng, Chen Shuqiang. Effects of Water-Saving Irrigation Cultivation Model on Rice Quality and Starch RVA Profiles [J]. Crops, 2021, 37(4): 86-92.
[10] Wu Zhifeng, Liu Kaili, Le Lihong, Chen Zhongping, Tang Shuangqin, Li Zujun, Han Ruicai, Zeng Yanhua, Zeng Yongjun, Pan Xiaohua, Shi Qinghua, Wu Ziming. Study on Relieving Cold Stress of Direct-Seeded Late Rice at Heading Stage by Chemical Control [J]. Crops, 2021, 37(3): 114-119.
[11] Meng Xiangyu, Ran Cheng, Liu Baolong, Zhao Zhexuan, Bai Jingjing, Geng Yanqiu. Effects of Straw Returning to Field and Nitrogen Application on Soil Nutrients and Rice Yield in Black Soil Areas of Northeast China [J]. Crops, 2021, 37(3): 167-172.
[12] Yi Zhenxie, Wang Yuanyuan, Gu Zihan, Shuai Zeyu, Tu Naimei, Chen Pingping. Study on the Feasibility of Alternative Planting of Rapeseed-Middle Rice to Double Cropping Rice in Cadmium Polluted Rice Area [J]. Crops, 2021, 37(3): 65-69.
[13] Gao Peng, Guo Meijun, Yang Xuefang, Dong Shuqi, Wen Yinyuan, Guo Pingyi, Yuan Xiangyang. Responses of Photosynthetic Fluorescence Parameters in Foxtail Millet and Maize Leaves under Nicosulfuron Stress [J]. Crops, 2021, 37(3): 70-77.
[14] Liu Ping, Shao Caihong, Zhang Honglin, Liu Guangrong. Effects of Dry-Wet Alternate Irrigation on Double Cropping Rice Yield and Quality during Late Development Stage under Seasonal Rain Condition [J]. Crops, 2021, 37(2): 153-159.
[15] Xing Yuan, He Zhonghua. Analysis of Characteristics of Rice Drought in Guizhou Province Based on Water Deficit Index [J]. Crops, 2021, 37(2): 191-199.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!