Crops ›› 2021, Vol. 37 ›› Issue (6): 46-50.doi: 10.16035/j.issn.1001-7283.2021.06.007

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Observation on Pollen Morphology of F-Type Male Sterile Lines and SQ-1 Induced Sterile Plants in Wheat by Scanning Electron Microscope

Ren Wenbin(), Wang Qian, Wu Cuicui, Xie Sangang   

  1. Institute of Cotton Research, Shanxi Agricultural University (Shanxi Academy of Agricultural Sciences), Yuncheng 044000, Shanxi, China
  • Received:2020-12-21 Revised:2021-03-01 Online:2021-12-15 Published:2021-12-16

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Abstract:

The morphology and structure of pollen grains of F-type male sterile lines and SQ-1 induced male sterile plants were observed by scanning electron microscope. By comparing the characteristics of pollen grain size, shape and germination aperture cover. The results showed that the polar axis length of pollen grains reached extremely significant level among normal development, SQ-1 induced and F-type male sterile lines. The pollen grains of SQ-1 induced sterile plants and F-type male sterile lines showed typical irregular abortion shape, and the germination aperture cover were sunken in different degrees. The shedding of germinating aperture cover was found at the same time.

Key words: Wheat, F-type male sterile line, Pollen grain, Germinal aperture

Table 1

Comparison of pollen grains of F-type male sterile lines, SQ-1 induced and normal development"

类型
Type		 花粉粒大小Pollen grain size (μm) 萌发孔大小
Germinal aperture
size (μm)		 萌发孔孔盖特征
Characteristic of germinal aperture cap
极轴长
Polar axis length		 赤道轴长
Equatorial axis length
正常发育
Normal development		 65.02±5.81aA 50.13±4.16abA （8.60±0.68）×（8.18±0.54） 圆润饱满挺直,向上凸起,高于孔环
舜麦1718 Shunmai 1718
（SQ-1诱导）		 46.53±3.95cC 40.40±3.18cB （8.04±0.56）×（7.22±0.51） 向下凹陷,低于孔环
晋麦84 Jinmai 84
（SQ-1诱导）		 43.81±3.55cC 39.62±2.82cB （7.88±0.58）×（7.05±0.61） 周围向下凹陷,孔盖凸起,高度平行于孔环
FA37-1 53.92±4.76bB 49.54±3.35bA （8.16±0.65）×（7.13±0.49） 向下凹陷,低于孔环
FA99-2 56.93±5.12bB 47.44±3.62bA （8.35±0.71）×（7.26±0.58） 向下凹陷,低于孔环
FA128-2 54.84±4.64bB 48.54±3.17bA （8.28±0.63）×（6.94±0.50） 向下凹陷,低于孔环

Fig.1

The morphological characteristics of the normal pollen grains and germinal aperture a and b represent the morphological characteristics of the pollen grains at 100× and 500×, respectively, c represents the morphological characteristics of germinal aperture at 10000×. The same below"

Fig.2

The morphological characteristics of the pollen grains and germinal aperture of Shunmai 1718 and Jinmai 84 induced by SQ-1"

Fig.3

The morphological characteristics of the pollen grains and germinal aperture of FA37-1, FA99-2, and FA128-2 male sterile lines"

Fig.4

The cap of germinal aperture felling off morphological characteristics of the normal pollen grains a represents the morphological characteristics of the pollen grains at 500×, b represents the morphological characteristics of germinal aperture at 5000×"

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