Crops ›› 2022, Vol. 38 ›› Issue (5): 221-228.doi: 10.16035/j.issn.1001-7283.2022.05.032

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Study of Rhizosphere Soil Microbial Changes of Atractylodes lancea Based on High-Throughput Sequencing

Xu Yan1(), Niu Junfeng1, Chen Lijun1, Wang Shiqiang1, Dong Zhongmin1,2, Wang Zhezhi1()   

  1. 1National Engineering Laboratory for the Development of Endangered Medicinal Materials in Northwest China, Xiʼan 710119, Shaanxi, China
    2Department of Biology, Saint Maryʼs University, Halifax B3H3C3, Nova Scotia, Canada
  • Received:2021-07-20 Revised:2022-06-17 Online:2022-10-15 Published:2022-10-19

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Abstract:

Atractylodes lancea is a perennial herb, and the traditional planting method is to plant in the same plot for several years, which leads to the obstruction of its growth and development and the occurrence of soil borne diseases. Rhizosphere microorganisms are considered as the second genome of plants and play an important role in plant growth and development. In order to analyze the influence of cultivated A.lancea on rhizosphere soil microorganisms and explore the relationship between the occurrence of A.lancea disease and microbial changes, taking the rhizosphere soil planted with A.lancea for three years in Xialiang town, Zhashui county, Shaanxi province and the soil which has never been planted with A.lancea as experimental materials, the microbial community was sequenced by high-throughput sequencing method. The findings revealed that after three years of cultivation, the bacteria and fungal communities in the rhizosphere soil of A.lancea changed in composition, with the fungal communityʼs diversity and richness increasing. The dominant bacterial groups were Proteobacteria, Actinobacteria and Acidobacteria, while the predominant fungal groups were Ascomycota, Mortierellomycota and Basidiomycota. After three years of cultivation, the rhizosphere soil microorganisms of A.lancea showed an overall trend of beneficial bacteria decreasing and disease bacteria increasing.

Key words: High-throughput sequencing, Atractylodes lancea, Soil microorganism, Change

Fig.1

The dilution curves of bacteria and fungi in soil before and after planting A.lancea"

Fig.2

The venn diagrams of bacteria and fungi before and after planting A.lancea"

Table 1

The diversity index of bacteria and fungi"

微生物Microbe 组名Group name 观察到的种类Observed species Shannon 辛普森指数Simpson Chao1指数Chao1 Ace指数Ace
细菌Bacteria ZX3 1969.33 8.82 0.993479 2103.02 2135.13
ZXCK 2160.00 9.12 0.994796 2312.60 2323.52
真菌Fungi ZX3 1175.00 6.25 0.944552 1272.30 1237.16
ZXCK 1158.33 6.16 0.934756 1281.40 1237.32

Fig.3

Distribution of soil bacteria phyla before and after planting A.lancea"

Fig.4

Distribution of soil fungal phyla before and after planting A.lancea"

Fig.5

Analysis of different species of soil bacteria before and after planting A.lancea P < 0.05 means significant difference, P < 0.01 means extremely significant difference, the same below"

Fig.6

Analysis of different species of soil fungi before and after planting A.lancea"

Fig.7

Analysis of dominant genes of soil bacteria before and after planting A.lancea"

Fig.8

Cluster heat map of the dominant genera of soil bacteria before and after planting A.lancea"

Fig.9

Analysis of the dominant genera of soil fungi before and after planting A.lancea"

Fig.10

Cluster heat map of the dominant genus of soil fungi before and after planting A.lancea"

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