Crops ›› 2023, Vol. 39 ›› Issue (4): 260-266.doi: 10.16035/j.issn.1001-7283.2023.04.037

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Breeding and Application of Elite Waxy Sorghum Male Sterile Line “Ji 5535A”

Chen Bingru(), Yu Miao, Shi Guishan, Wang Jianghong, Tang Yujie, Xu Chen, Li Haiqing, Xu Ning, Zhou Ziyang(), Wang Nai()   

  1. Institute of Crop Germplasm Resources, Jilin Academy of Agricultural Sciences, Changchun 130033, Jilin, China
  • Received:2022-02-11 Revised:2022-04-01 Online:2023-08-15 Published:2023-08-15

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Abstract:

To breed elite waxy sorghum male sterile lines for early-maturing area in China, Ji 2055B was selected as female parent, a elite non-waxy sorghum maintainer line with strong combining ability, strong disease resistance and excellent grain quality, a dwarf waxy material from the progeny of non-waxy maintainer line 406B and waxy maintainer line ICS-34B was used as waxy donor. Through crossing of one generation, selfing of ten generations and backcrossing of five generations in five years, the elite waxy sorghum male sterile lines Ji 5535A were successfully bred. Ji 5535A had good grain quality, its amylopectin content was 99.17%, crude protein content was 14.29%, crude fat content was 3.15%, tannin content was 0.01%, lysine content was 0.33%, and 1000-grain weight reached 27.2g. Ji 5535A had strong disease resistance, high resistance to sorghum head smut, resistance anthracnose and target leaf spot, moderate resistance to stalk rot. The waxy allele of Ji 5535A was wxb by molecular marker analysis. High-quality forage and edible sorghum hybrids could be bred through Ji 5535A and low tannin restorer lines, and high-quality brewing sorghum hybrids could be bred through Ji 5535A and high tannin restorer lines. The resulted revealed that Ji 5535A was an excellent male sterile line for cultivating brewing, grain forage and edible sorghum hybrids. Ji 5535A expanded the genetic basis of waxy male sterile lines and provided excellent germplasm for the improvement of sorghum male sterile lines in China. Meanwhile, it improved the yield and quality of waxy sorghum hybrids in early maturing area, and promoted the cultivation of brewing, forage and edible sorghum hybrids.

Key words: Sorghum, Waxy, Male sterile lines, Ji 5535A

Table 1

The primers of allelic variation of Waxy"

引物名称
Primer name		 引物序列
Primer sequence		 扩增产物
Amplification product (bp)		 检测方法
Test method		 功能
Function
Wxa-F CGTGGCGAGATCAAACTCTA 615或523 电泳 用于区分Wxa和野生型及其他3种突变[18-19]，当扩增片段为615bp时，为Wxa。当扩增片段为523bp时，为Wx-WT、Wxb、Wxc和Wxd。
Wx-F GGCCTGGATTCAATGTTCTT
Wx-R CTGGTTGTCCTTGTAG
Wx-F2 GGCATCTACAAGGACGCAAAG 990 测序 通过测序区分Wx-WT、Wxb、Wxc和Wxd。
Wx-R2 CGCGTTGAACTTGACCAC

Fig.1

The breeding process of Ji 5535A"

Fig.2

Comparison of genetic distance between Ji 5535A, Ji 2055A, Ji 521A and backbone restorer lines"

Fig.3

The results of Waxy sequencing of Ji 5535A, Ji 2055A and Ji 521A (a) the agarose electrophoresis result showed that the amplified fragment of Ji 5535A was 523bp by Wxa-F、Wx-F and Wx-R primers, the Waxy allele mutation of Ji 5535A was not Wxa and was one of Wx-WT、Wxb、Wxc and Wxd. (b) Sequenced after PCR amplification using the primers Wx-F2 and Wx-R2, the sequencing result showed that the Waxy allele mutation of Ji 5535A was Wxb type and 804 site G mutated to T in CDS"

Table 2

Comparison of quality traits between Ji 5535A and other backbone waxy sterile lines"

种质名称
Germplasm name		 总淀粉含量
Starch content (%)		 支链淀粉含量
Amylopectin content (%)		 粗蛋白含量
Protein content (%)		 单宁含量
Tannin content (%)		 千粒重
1000-grain weight (%)
吉5535A Ji 5535A 69.53 99.17 14.29 0.01 27.2
45A 75.56 98.12 8.23 0.41 24.1
13163A 73.01 99.18 12.45 0.03 25.3
72A 73.89 96.00 9.35 0.17 22.2
张2A Zhang 2A 73.55 96.99 9.42 0.08 21.3
永糯2A Yongnuo 2A 70.88 98.85 9.30 0.13 20.6

Table 3

Comparison of quality traits between waxy sorghum hybrids bred by Ji 5535A and main waxy hybrids from different ecological regions"

种质名称
Germplasm name		 总淀粉含量
Starch content (%)		 支链淀粉含量
Amylopectin content (%)		 蛋白含量
Protein content (%)		 单宁含量
Tannin content (%)		 脂肪
Fat content (%)
吉杂238 Jiza 238 75.47 99.37 9.91 1.10 3.21
吉糯杂3号Jinuoza 3 76.25 98.10 9.23 1.02 4.18
吉糯杂9号 Jinuoza 9 75.80 97.44 8.69 1.09 2.29
晋糯杂3号Jinnuoza 3 74.38 97.00 9.56 1.01 3.44
辽粘3号Liaonian 3 78.09 91.29 8.14 1.47 3.54
辽糯11 Liaonuo 11 76.26 93.78 8.82 1.04 3.99
金糯粱1号Jinnuoliang 1 73.86 98.30 8.63 1.13 3.86
川糯粱1号Chuannuoliang 1 73.29 98.64 8.57 1.54 3.75
机糯粱2号Jinuoliang 2 73.43 99.02 9.70 1.31 4.16
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