小麦脱植基相关基因在春性小麦叶片叶绿素降解过程中的作用分析

doi:10.16035/j.issn.1001-7283.2022.02.012

摘要/Abstract

摘要：

小麦叶片的衰老会导致产量的损失,而叶绿素降解是小麦叶片衰老的明显特征,分析小麦叶绿素降解过程中脱植基反应的相关基因叶绿素酶（TaCLH）和脱镁叶绿素水解酶（TaPPH）在春性小麦叶片衰老过程中的作用,为解析小麦叶绿素降解的分子机制提供参考。以10个春性小麦品种为参试材料,对衰老过程中TaCLH和TaPPH的相对表达量进行测定,结合不同品种在开花后不同时期的相对叶绿素含量（SPAD）、功能绿叶面积（GLAD）和叶绿素荧光参数的变化规律,研究叶片衰老过程中TaCLH和TaPPH与SPAD、GLAD和叶绿素荧光参数的相关关系。结果表明,TaPPH的相对表达量与GLAD、SPAD及叶绿素荧光参数[ETR、F_v/F_m、Y(Ⅱ)]等生理指标之间存在极显著负相关关系,与TaCLH相对表达量存在极显著正相关关系,表明TaPPH在春小麦叶绿素降解过程的脱植基反应中起主要作用。

关键词: 小麦, 叶绿素, 荧光参数, TaCLH, TaPPH, 基因表达

Abstract:

Leaf senescence of wheat leads to yield loss, and chlorophyll degradation is an important feature of wheat leaf senescence. The purpose of this study is to explore the role of chlorophyll degradation-related genes chlorophyllase (TaCLH) and pheophytin pheophorbide hydrolase (TaPPH) in the senescence process of spring wheat leaves, and to provide a reference for analyzing the molecular mechanism of chlorophyll degradation in wheat. Ten spring wheats were used as test materials. The relative expression of TaCLH and TaPPH in the flag leaves during the aging process were measured, the relative chlorophyll content (SPAD value) and functional leaf area (GLAD) of different wheat varieties at different stages after anthesis as well as the changes in chlorophyll fluorescent parameters were analyzed to explore the correlation between TaCLH and TaPPH with SPAD, GLAD and chlorophyll fluorescent parameters during leaf senescence. The results showed that there were extremely significant negative correlations between TaPPH relative expression level with SPAD, GLAD and chlorophyll fluorescence parameters [ETR, F_v/F_m, Y(II)], and there was a highly significant positive correlation between the relative expression levels of TaCLH and TaPPH. This indicated that TaPPH played a major regulatory role in the flag leaf senescence process after anthesis in spring wheat.

Key words: Wheat, Chlorophyll, Fluorescence parameters, TaCLH, TaPPH, Gene expression

赵锴, 晋秀娟, 孙丽丽, 闫荣岳, 卢娟, 郭峰, 史雨刚, 孙黛珍. 小麦脱植基相关基因在春性小麦叶片叶绿素降解过程中的作用分析[J]. 作物杂志, 2022 (2): 81–88

Zhao Kai, Jin Xiujuan, Sun Lili, Yan Rongyue, Lu Juan, Guo Feng, Md Ashraful Islam, Shi Yugang, Sun Daizhen. The Role of Wheat Deplantation-Related Genes in Degradation of Chlorophyll in Spring Wheat Leaves[J]. Crops, 2022(2): 81–88

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指标 Index	品种 Variety	特征参数Characteristic parameter
指标 Index	品种 Variety	MRS	TMRS (d)	T_s (d)	T₀ (d)
SPAD	晋麦2148	5.48ab	23.97ef	15.60ab	27.07d
	宁春13号	4.38bc	24.90cdef	14.21ab	28.88cd
	内麦19号	3.62cd	23.72f	11.79bc	28.22cd
	博爱7023	5.02bcd	26.01bc	16.56a	29.51abc
	生选3号	5.39ab	25.46cd	16.78a	28.67bcd
	川麦22	6.72a	27.31a	19.11a	30.34a
	绵阳15号	4.51bcd	27.10ab	17.17a	30.77a
	新春8号	3.93cd	25.56cd	12.54bc	30.48a
	宁春4号	3.58cd	24.59def	11.08c	29.77abc
	青春5号	3.44d	25.22cde	12.72bc	29.92ab
GLAD值 GLAD value	晋麦2148	0.74a	27.28abc	15.78a	31.55abc
GLAD值 GLAD value	宁春13号	0.75a	28.16a	16.68a	32.42ab
	内麦19号	0.80a	27.16abc	16.58a	31.09abc
	博爱7023	0.85a	24.57ef	14.59a	28.27bc
	生选3号	0.76a	23.94f	13.15a	27.97c
	川麦22	0.66a	27.29ab	13.89a	32.32a
	绵阳15号	0.60a	27.18ab	13.69a	32.25a
	新春8号	0.70a	26.33bcd	13.94a	30.97abc
	宁春4号	0.62a	25.73cde	12.13a	30.90abc
	青春5号	0.65a	25.59de	12.29a	30.63abc

指标 Index	类群 Group	特征参数Characteristic parameter
指标 Index	类群 Group	MRS	TMRS (d)	T_s (d)	T₀ (d)
SPAD	Ⅰ	5.42	25.97	17.04	29.27
	Ⅱ	3.64	24.77	12.03	29.60
	Ⅲ	4.38	24.90	14.21	28.88
GLAD值 GLAD value	Ⅰ	0.79	26.79	15.91	30.83
GLAD值 GLAD value	Ⅱ	0.73	25.14	13.55	29.47
	Ⅲ	0.63	26.45	13.00	31.53

指标Index	Y(II)	F_v/F_m	ETR	q_P	GLAD值GLAD value	SPAD	TaCLH	TaPPH
Y(II)	1
F_v/F_m	0.952^**	1
ETR	0.919^**	0.928^**	1
q_P	0.063	0.014	0.083	1
GLAD	0.919^**	0.914^**	0.840^**	0.054	1
SPAD	0.913^**	0.945^**	0.893^**	0.010	0.905^**	1
TaCLH	-0.075	-0.076	-0.006	0.100	-0.061	-0.089	1
TaPPH	-0.587^**	-0.564^**	-0.450^**	0.121	-0.591^**	-0.575^**	0.391^**	1