盐碱胁迫下不同基因型绿豆对外源BR的响应

doi:10.16035/j.issn.1001-7283.2025.05.008

摘要/Abstract

摘要： 以绿丰2号、嫩绿4号和中绿27作为试验材料，探究了0.5、1.0、1.5和2.0 mg/L的油菜素内酯（brassinosteroids，BR）对盐碱胁迫下幼苗的调控效应。结果表明，盐碱环境下绿豆幼苗根部生长缓慢，土壤养分吸收能力下降，幼苗植株光合色素含量下降，光合速率降低，导致干物质积累量减少。喷施BR处理能够显著提高绿豆幼苗叶绿素相对含量，1.5 mg/L处理时地下部分根系总长和根表面积均显著增加；同时使得植株体内超氧化物歧化酶（SOD）、过氧化物酶（POD）和过氧化氢酶（CAT）活性提升，可溶性蛋白、脯氨酸和可溶性糖等渗透调节物质含量增加，显著降低了盐碱环境所带来的损伤。综上所述，盐碱胁迫下外源BR通过提高幼苗植株体内抗氧化酶活性及渗透调节物质含量，降低绿豆幼苗植株所受到的伤害。

关键词: 绿豆, 盐碱胁迫, 2,4-油菜素内酯, 幼苗生长, 叶绿素含量, 抗氧化酶活性

Abstract:

This study investigated the regulatory effects of exogenous brassinosteroids (BR) on mung bean seedlings under saline-alkali stress. Three genotypes (?Lüfeng 2?, ?Nenlü 4?, and ?Zhonglü 27?) were used, with BR applied at concentrations of 0.5, 1.0, 1.5, and 2.0 mg/L. The results showed that saline-alkali stress inhibited root growth, reduced nutrient uptake, decreased photosynthetic pigment content, and lowered photosynthetic capacity, leading to reduced dry matter accumulation. The content of photosynthetic pigments in seedlings stage decreased, and their photosynthetic capacity weakened, resulting in a reduction in dry matter accumulation. Spraying BR treatment could significantly increase the chlorophyll content of mung bean seedlings stage. Under the treatment of 1.5 m/L, the total root length and root surface area of the underground part significantly increased. Meanwhile, by regulating the enhancement of the activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) in the plant body, and the increase in the contents of osmotic adjustment substances such as soluble protein, proline, and soluble sugar, the damage caused by the saline-alkali environment was significantly reduced. This treatment also enhanced the activity of antioxidant enzymes and increased the content of osmotic adjustment substances, thereby greatly reducing the damage caused by the saline-alkali environment.

Key words: Mung bean, Saline-alkali stress, Brassinosteroids, Seedling growth, Chlorophyll content, Antioxidant enzyme activity

张金东, 王成, 卢环, 曾玲玲, 张巩亮, 孙浩月, 刘悦, 杨贺麟, 侯晓敏. 盐碱胁迫下不同基因型绿豆对外源BR的响应[J]. 作物杂志, 2025 (5): 54–60

Zhang Jindong, Wang Cheng, Lu Huan, Zeng Lingling, Zhang Gongliang, Sun Haoyue, Liu Yue, Yang Helin, Hou Xiaomin. Response of Mung Bean Genotypes to Exogenous Brassinosteroids under Saline-Alkali Stress[J]. Crops, 2025(5): 54–60

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品种 Variety	处理 Treatment	株高 Plant height (cm)	干物质积累量 Dry matter accumulation (g)
绿丰2号 Lüfeng 2	CK	17.09±0.53a	0.30±0.01a
	T1	9.46±0.30d	0.17±0.01e
	T2	12.96±0.22c	0.19±0.01de
	T3	12.76±0.26c	0.24±0.01b
	T4	14.86±0.76b	0.21±0.01cd
	T5	13.61±0.18bc	0.23±0.01bc
嫩绿4号 Nenlü 4	CK	17.79±0.61a	0.37±0.01a
	T1	10.48±0.33d	0.19±0.01c
	T2	13.00±0.17c	0.21±0.02bc
	T3	13.77±0.44bc	0.23±0.01b
	T4	15.11±0.40b	0.21±0.01bc
	T5	12.53±0.49c	0.23±0.01b
中绿27 Zhonglü 27	CK	17.79±0.61a	0.49±0.01a
	T1	18.17±0.46a	0.20±0.01d
	T2	11.21±0.29d	0.21±0.01cd
	T3	13.32±0.30c	0.29±0.01b
	T4	13.80±0.39c	0.26±0.01b
	T5	15.40±0.38b	0.25±0.02bc
F值 F-value	品种	4.95^*	93.78^**
	浓度	91.52^**	264.00^**
	品种×浓度	2.25^*	19.35^**

品种 Variety	处理 Treatment	根系总长 Total root length (mm)	根系干重 Root dry weight (g)	根体积 Root volume (cm³)	根表面积 Root surface area (cm²)
绿丰2号Lüfeng 2	CK	447.12±7.59a	0.06±0.01a	0.04±0.01a	39.14±2.58a
	T1	93.63±3.35e	0.01±0.01c	0.01±0.01d	12.19±1.97d
	T2	105.37±6.13de	0.02±0.01bc	0.02±0.01c	15.25±0.90cd
	T3	133.36±6.40cd	0.02±0.01b	0.02±0.01b	16.32±1.65cd
	T4	213.92±14.10b	0.02±0.01bc	0.02±0.01c	21.63±0.25b
	T5	151.20±8.72c	0.02±0.01b	0.02±0.01b	18.55±0.56bc
嫩绿4号Nenlü 4	CK	112.75±16.58a	0.06±0.01a	0.04±0.01a	37.61±2.22a
	T1	119.10±6.97d	0.02±0.01c	0.01±0.01c	15.10±1.65d
	T2	143.17±7.56d	0.02±0.01c	0.02±0.01c	17.11±0.86cd
	T3	147.76±4.39c	0.02±0.01c	0.02±0.01b	20.02±0.95bc
	T4	201.82±7.62b	0.02±0.01c	0.02±0.01c	22.99±1.25b
	T5	127.76±2.58cd	0.03±0.01b	0.02±0.01b	19.41±0.02bcd
中绿27 Zhonglü 27	CK	391.14±5.18a	0.10±0.01a	0.06±0.01a	59.62±7.14a
	T1	104.75±1.68d	0.02±0.01c	0.02±0.01b	14.75±0.66c
	T2	112.63±4.55d	0.04±0.01b	0.02±0.01b	19.89±0.77bc
	T3	187.42±3.21b	0.04±0.01b	0.03±0.01b	21.97±0.67bc
	T4	188.40±9.74b	0.03±0.01bc	0.02±0.01b	27.15±1.29b
	T5	151.46±9.23c	0.03±0.01bc	0.04±0.02ab	21.68±0.16bc
F值F-value	品种	8.85^**	173.86^**	8.68^**	41.34^***
	浓度	1220.19^**	392.65^**	26.80^**	195.11^**
	品种×浓度	14.30^**	20.05^**	1.26	9.86^**

品种 Variety	处理 Treatment	可溶性糖 Soluble sugar (mg/g)	可溶性蛋白 Soluble protein (mg/g)	脯氨酸 Proline (μg/g)
绿丰2号 Lüfeng 2	CK	3.62±0.08c	17.11±0.98ab	11.03±0.32d
	T1	3.96±0.10bc	11.00±0.39d	11.28±0.24d
	T2	4.66±0.26b	16.19±0.96ab	12.00±0.39cd
	T3	4.27±0.38bc	14.62±0.10bc	17.19±0.43b
	T4	6.10±0.48a	12.86±0.54cd	12.97±0.22c
	T5	4.58±0.18bc	19.04±1.61a	18.91±0.48a
嫩绿4号 Nenlü 4	CK	4.12±0.14c	18.36±0.12a	11.95±0.07d
	T1	4.36±0.15c	11.88±0.77c	11.92±0.14d
	T2	5.18±0.11b	15.02±0.98b	13.45±0.23c
	T3	5.13±0.14b	12.36±0.35c	16.44±0.13b
	T4	5.91±0.24a	12.44±0.25c	12.61±0.09cd
	T5	4.69±0.25bc	17.12±0.93ab	20.77±0.62a
中绿27 Zhonglü 27	CK	4.88±0.10c	16.36±1.02ab	10.84±0.19d
	T1	5.05±0.05c	13.03±0.84bc	11.80±0.10d
	T2	5.35±0.06b	15.74±1.23ab	15.03±0.35c
	T3	5.45±0.10b	15.06±1.58ab	21.81±0.21a
	T4	6.80±0.05a	11.51±0.20c	14.72±0.28c
	T5	4.40±0.04d	16.87±0.82a	20.61±0.44b
F值 F-value	品种	46.76^**	1.61	112.08^**
	浓度	80.99^**	51.54^**	800.63^**
	品种×浓度	5.08^**	3.32^**	31.31^**