检索
E-mail
RSS
高级检索 图表检索

当期目录 我要投稿 过刊浏览
高级检索 图表检索

作物杂志,2019, 第3期: 185–191 doi: 10.16035/j.issn.1001-7283.2019.03.031

• 种子科技 • 上一篇    下一篇

碱化橄榄油加工废弃液对玉米种子萌发及幼苗生长的影响

叶文斌1,2,3,何玉鹏1,2,王昱1,2,王瀚1,2,赵庆芳4   

  1. 1 陇南师范高等专科学校农林技术学院,742500,甘肃成县
    2 陇南特色农业生物资源研究开发中心,742500,甘肃成县
    3 甘肃省高等学校农村电商人才培养重点实验室,742500,甘肃成县
    4 西北师范大学生命科学学院,730030,甘肃兰州
  • 收稿日期:2018-09-30 修回日期:2019-04-16 出版日期:2019-06-15 发布日期:2019-06-12
  • 作者简介:叶文斌,副教授,研究方向为天然产物化学与植物生理生态
  • 基金资助:
    甘肃省自然科学基金(1107RJZK243);甘肃省教育厅项目(2015B-148);陇南市科技计划项目(2016-09)

Effects of Alkalized Olive Oil Processing Liquid Wastes on Seed Germination and Seedling Growth of Zea mays L.

Ye Wenbin1,2,3,He Yupeng1,2,Wang Yu1,2,Wang Han1,2,Zhao Qingfang4   

  1. 1 School of Agriculture and Forestry Technology, Longnan Normal College, Chengxian 742500, Gansu, China
    2 Center for Research & Development of Longnan Characteristic Agro-Bioresources, Chengxian 742500, Gansu, China;
    3 Key Laboratory of Gansu University for Rural E-Commerce Talents Training, Chengxian 742500, Gansu, China
    4 College of Life Sciences, Northwest Normal University, Lanzhou 730030, Gansu, China
  • Received:2018-09-30 Revised:2019-04-16 Online:2019-06-15 Published:2019-06-12

RichHTML

6

PDF (PC)

196

摘要：

通过研究橄榄油加工废弃液对玉米种子萌发及幼苗生长的影响,为橄榄油废弃物利用以及农田环境保护提供参考。将橄榄油加工废弃液（10%~60%）用0.10% Na2CO3碱化处理,考察其对玉米种子萌发、幼苗生长以及生理生化特性的影响。30%碱化废弃液能显著促进玉米种子萌发和幼苗生长,随废弃液浓度升高,种子萌发率显著降低,幼苗生长和生物量受阻,幼苗整齐度降低,叶片O2 -·产生速率先升高后降低,H2O2、游离脯氨酸、可溶性糖和丙二醛含量显著增加,超氧化物歧化酶（SOD）、过氧化物酶（POD）、过氧化氢酶

关键词: 橄榄油加工废弃液, 碱化, 玉米, 种子萌发, 幼苗生长, 生理生化特性

Abstract:

The purpose of this study is to study the effects of waste liquid from olive oil processing on the seed germination and seedling growth of Zea mays L. so as to provide references for the utilization of olive oil waste and farmland environmental protection. Olive oil processing waste liquid (10%-60%) alkalified with 0.10% Na2CO3, was applied to investigate the effects on seed germination, seedling growth and physiological and biochemical characteristics in maize. Alkalinized waste solution (30%) could significantly promote maize seed germination and seedling growth. With the increasing concentration of alkaline waste liquid, the germination rate was significantly reduced and seedling growth was blocked, biomass accumulation was limited, seedling uniformity was also decreased. O2 -· producing rate and H2O2 content in the leaves increased significantly and the accumulation of free proline, soluble sugar and malondialdehyde in the seedlings was obvious. The activities of superoxide dismutase, peroxidase, catalase, and ascorbate peroxidase in the seedlings first increased and then decreased, which showed a trend of high concentration inhibition and low concentration induced enhancement. These results suggested that 30% alkalized waste liquid could effectively promote seed germination and seedling growth of maize. The results of this research provide important reference values for the processing and application of olive oil processing waste liquid.

Key words: Olive oil processing waste liquid, Alkalization, Maize, Seed germination, Seedlings growth, Physiological and biochemical characteristics

表1

不同浓度Na2CO3处理的橄榄油加工废弃液主要组成和理化特性"

指标Index Na2CO3浓度Na2CO3concentration (%)
CK(0) 0.05 0.10 0.20
pH 4.66 5.39 6.96 8.36
导电性Conductivity (mS/cm) 5.56 6.14 6.84 7.65
干物质Dry matter (g/L) 65.23 66.53 66.97 67.13
有机质Organic matter (g/L) 45.36 45.86 46.12 46.81
油脂Oil (g/L) 1.63 1.14 0.93 0.81
多酚Polyphenols (g/L) 1.25 1.04 0.75 0.58
糖类Sugar (g/L) 2.19 2.28 2.39 2.44
有机酸Organic acid (g/L) 0.73 0.64 0.52 0.41
总氮Total nitrogen (g/L) 0.35 0.36 0.45 0.49
Na (g/L) 0.04 0.05 0.06 0.12
K (g/L) 0.76 0.76 0.76 0.76
Mg (g/L) 0.08 0.08 0.08 0.08
Cl (g/L) 0.59 0.59 0.59 0.59

图1

不同浓度橄榄油加工废弃液和0.10% Na2CO3对玉米种子萌发的影响 CK(0)为蒸馏水处理,CK(0.1)为0.10%的Na2CO3处理,下同"

图2

不同浓度碱化橄榄油加工废弃液对玉米种子萌发的影响 K(10)~K(60)指经0.10% Na2CO3处理的橄榄油加工废弃液,浓度分别为10%、20%、30%、40%、50%、60%,下同"

图3

不同浓度碱化橄榄油加工废弃液对玉米幼苗生长的影响"

图4

不同浓度碱化橄榄油加工废弃液对玉米幼苗整齐度的影响"

图5

不同浓度碱化橄榄油加工废弃液对玉米幼苗中可溶性糖、游离脯氨酸和MDA含量的影响"

图6

不同浓度碱化橄榄油加工废弃液对玉米幼苗中O2-·产生速率和H2O2含量的影响"

图7

不同浓度碱化橄榄油加工废弃液对玉米幼苗中SOD、POD、CAT和APX活性的影响"

[1] Kalua C M, Allem M S, Bedgood Jr D R , et al. Olive oil volatile compounds,flavour development and quality:a critical review. Food Chemistry, 2007,100(1):273-286.
doi: 10.1016/j.foodchem.2005.09.059
[2] 刘娜, 白万明, 韩锐 , 等. 橄榄油加工废弃物中的活性成分及其综合利用技术研究进展. 中国油脂, 2016,41(5):84-88.
[3] 邹光友 . 油橄榄加工副产物的综合开发利用. 广州食品工业科技, 1989,5(4):12-15.
[4] 王成章, 陈强, 罗建军 , 等. 中国油橄榄发展历程与产业展望. 生物质化学工程, 2013,47(3):41-46.
[5] Zbakh H, Abbassi A E . Potential use of olive mill waste water in the preparation of functional beverages:a review. Journal of Functional Foods, 2012,4(1):53-65.
doi: 10.1016/j.jff.2012.01.002
[6] 叶文斌, 樊亮, 王瀚 , 等. 橄榄油加工液体废弃物对武都红芪种子萌发及幼苗生理特性的影响. 广东农业科学, 2017,44(1):30-35.
[7] Piotrowska A, Iamarino G, Rao M A , et al. Short-term effects of olive mill waste water (OMW) on chemical and biochemical properties of a semiarid Mediterranean soil. Soil Biology and Biochemistry, 2006,38(3):600-610.
doi: 10.1016/j.soilbio.2005.06.012
[8] Roberto A, Esposito A, Baruzzi G . Use of olive mill waste mix as peat surrogate in substrate for strawberry soilless cultivation. International Biodeterioration & Biodegradation, 2010,64(7):670-675.
[9] 刘娜, 白万明, 苏玲玲 , 等. 油橄榄加工废液对小麦和玉米种子萌发及相关酶活性的影响. 西北农业学报, 2016,36(3):565-572.
[10] 叶文斌, 何玉鹏 . 橄榄油加工废液碱化后对小麦种子萌发及幼苗生长的影响. 宁夏师范学院学报, 2018,39(7):57-63.
[11] 叶文斌, 樊亮, 贠汉伯 . Pb 2+对甘肃纹党种子萌发和幼苗生理特性的影响 . 西北农业学报, 2012,21(12):142-148.
[12] 叶文斌, 樊亮, 叶建龙 . Zn盐和干旱胁迫对纹党种子萌发和幼苗生理特性的影响. 干旱地区农业研究, 2015,33(2):106-112,134.
[13] 叶文斌 . Zn 2+对纹党种子萌发和幼苗渗透调节物质积累与活性氧代谢的影响 . 种子, 2014,33(2):43-46.
[14] 中国科学院上海植物生理研究所, 上海市植物生理学会. 现代植物生理学实验指南. 北京: 科学出版社,1999:95-96,303,305,127.
[15] Roshchina V V, Roshchina V D . The Excretory Function of Higher Plant. New York:Spingerverlag, 1993: 213-215.
[16] Quan W L, Liu X, Wang H Q , et al. Comparative physiological and transcriptional analyses of two contrasting drought tolerant alfalfa varieties. Frontiers in Plant Science, 2015,6:1-16.
[17] Hosseini Boldaji S A, Khavari-Nejad R H, Hassan Sajedi H , et al. Water availability effects on antioxidant enzyme activities,lipid peroxidation,and reducing sugar contents of alfalfa (Medicago sativa L.). Acta Physiologiae Plantarum, 2012,34(3):1177-1186.
doi: 10.1007/s11738-011-0914-6
[18] Kang Y, Han Y, Torres-jerez I , et al. System responses to long-term drought and re-watering of two contrasting alfalfa varieties. Plant Journal, 2011,68(5):871-889.
doi: 10.1111/j.1365-313X.2011.04738.x
[19] Farooq M, Wahid A, Kobayashi N , et al. Plant drought stress:Effects,mechanisms and management. Agronomy for Sustainable Development, 2009,29(1):185-212.
doi: 10.1051/agro:2008021
[20] Griffiths H, Parry M A . Plant responses to water stress. Annals of Botany, 2002,89(7):801-802.
[21] Mittler R . Oxidative stress,antioxidants and stress tolerance. Trends in Plant Science, 2002,7(9):405-410.
doi: 10.1016/S1360-1385(02)02312-9
[22] Karatas I, Özturk L, Demir Y , et al. Alterations in antioxidant enzyme activities and proline content in pea leaves under long-term drought stress. Toxicology & Industrial Health, 2014,30(8):693-700.
[1] 付景,孙宁宁,刘天学,马俊峰,杨豫龙,赵霞,穆心愿,李潮海. 穗期高温对玉米子粒灌浆生理及产量的影响[J]. 作物杂志, 2019, (3): 118–125
[2] 鲁守平,张华,孟昭东,穆春华. 利用分子标记技术对玉米自交系子粒油分的改良研究[J]. 作物杂志, 2019, (3): 24–28
[3] 范惠玲,白生文,朱雪峰,李振洲,秦明岗,何志军. 油菜及其近缘种种子萌发期耐盐碱性差异[J]. 作物杂志, 2019, (3): 178–184
[4] 董哲,杨武德,张美俊,朱洪芬,王超. 基于高光谱遥感的玉米叶片SPAD值估算模型研究[J]. 作物杂志, 2019, (3): 126–131
[5] 吴建忠,李绥艳,林红,马延华,潘丽艳,李东林,孙德全. 青贮玉米品质性状遗传变异及主成分分析[J]. 作物杂志, 2019, (3): 42–48
[6] 史亚兴,董会,卢柏山,赵久然,樊艳丽,徐丽,俞嫒年. 糯玉米不同采收期子粒降水及糊化特性研究[J]. 作物杂志, 2019, (3): 112–117
[7] 任洪雷,李春霞,龚士琛,李国良,扈光辉,王明泉,杨剑飞. 利用SPSS实现玉米杂交种主要农艺性状与产量的相关和通径分析[J]. 作物杂志, 2019, (3): 86–90
[8] 代希茜,詹和明,崔兴洪,赵银月,单丹丹,张亮,王铁军. 玉米大豆间作种植密度耦合数学模型及其优化方案研究[J]. 作物杂志, 2019, (2): 128–135
[9] 张冬梅,黄学芳,姜春霞,张伟,王晓娟,刘化涛,闫六英,刘恩科,翟广谦. 冷凉区旱地玉米微垄覆膜土壤水热及产量效应研究[J]. 作物杂志, 2019, (2): 115–121
[10] 张宇飞,刘立志,马昱萱,王晓纯,戴建军. 耕作和秸秆还田方式对玉米产量及钾素积累转运的影响[J]. 作物杂志, 2019, (2): 122–127
[11] 史亚兴,徐丽,赵久然,卢柏山,樊艳丽. 中国糯玉米产业优势及在“一带一路”发展中的机遇[J]. 作物杂志, 2019, (2): 15–19
[12] 孙丽娟,赵志宏,贺娟,王步军. 我国鲜食玉米相关标准问题分析及对策[J]. 作物杂志, 2019, (2): 46–50
[13] 唐会会,许艳丽,王庆燕,马正波,李光彦,董会,董志强. 叶面喷施5-氨基乙酰丙酸对不同密度春玉米生长特性和产量的影响[J]. 作物杂志, 2019, (2): 136–141
[14] 闫晶,姬文秀,石贤吉,朱诗苗,李虎林. 镉胁迫对烟草种子萌发和烟苗生长发育的影响[J]. 作物杂志, 2019, (2): 142–149
[15] 张翼飞,于崧,王玉凤,杨克军,郭庄园,李瑶,孙逸珊,贾文宝,张亚凤. 促进玉米子粒脱水的农艺措施研究进展[J]. 作物杂志, 2019, (1): 1–8
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] 房裕东,韩天富. 作物快速育种技术研究进展[J]. 作物杂志, 2019, (2): 1 –7 .
[2] 张萌,芶久兰,魏全全,陈龙,何佳芳. 不同生物有机肥对贵州高海拔春马铃薯生长及土壤肥力的影响[J]. 作物杂志, 2019, (3): 132 –136 .
[3] 付景,孙宁宁,刘天学,马俊峰,杨豫龙,赵霞,穆心愿,李潮海. 穗期高温对玉米子粒灌浆生理及产量的影响[J]. 作物杂志, 2019, (3): 118 –125 .
[4] 权宝全,吕瑞洲,王贵江,任杰成. 薯块膨大中期不同栽培措施对甘薯生长发育及产量的影响[J]. 作物杂志, 2019, (3): 158 –161 .
[5] 鲁守平,张华,孟昭东,穆春华. 利用分子标记技术对玉米自交系子粒油分的改良研究[J]. 作物杂志, 2019, (3): 24 –28 .
[6] 张自强,王良,白晨,张惠忠,李晓东,付增娟,赵尚敏,鄂圆圆,张辉,张必周. 104份甜菜种质资源主要农艺性状分析[J]. 作物杂志, 2019, (3): 29 –36 .
[7] 马名川,刘龙龙,张丽君,崔林,周建萍. EMS诱变刺荞的形态突变体鉴定与分析[J]. 作物杂志, 2019, (3): 37 –41 .
[8] 范惠玲,白生文,朱雪峰,李振洲,秦明岗,何志军. 油菜及其近缘种种子萌发期耐盐碱性差异[J]. 作物杂志, 2019, (3): 178 –184 .
[9] 王永刚,姬铭泽,赵旭涵,于立河,薛盈文. 播期对白燕7号在黑龙江省中西部地区产量的影响[J]. 作物杂志, 2019, (3): 106 –111 .
[10] 董哲,杨武德,张美俊,朱洪芬,王超. 基于高光谱遥感的玉米叶片SPAD值估算模型研究[J]. 作物杂志, 2019, (3): 126 –131 .