不同夜间温度处理对马铃薯试管薯及块茎形成相关基因表达的影响

doi:10.16035/j.issn.1001-7283.2022.03.013

摘要/Abstract

摘要：

为探究不同夜间温度处理对马铃薯试管薯形成的影响,以7个马铃薯品种或材料的试管苗为试材,在昼/夜温度分别为23℃/13℃、23℃/18℃和23℃/23℃条件下进行试管薯的诱导培养,调查记录试管薯初始结薯时间、结薯率和结薯方式变化,并分析不同夜间温度处理下试管薯初始形成植株及同期未形成试管薯植株参与块茎形成相关基因的表达量变化。结果表明,对于大多数基因型,与其他处理相比,23℃/13℃处理显著延长试管薯初始结薯时间,23℃/23℃处理降低结薯率,而23℃/18℃处理结薯较早且试管薯结薯率平均达84.3%,最利于试管薯形成。夜间温度可通过影响生物钟、糖代谢和光周期通路中StFKF1、StCO1和StSWEET1等相关基因的表达而调控块茎形成。

关键词: 马铃薯, 试管薯形成, 夜间温度, 初始结薯时间, 基因表达

Abstract:

In order to investigate the effects of different night-time temperature treatments on in vitrotuberization in potato, the initial time, percentage and type of in vitrotuberization were recorded using seven potato varieties or materials induced cultured at day/night temperature of 23℃/13℃, 23℃/18℃ and 23℃/23℃. The expression changes in tuberization-related genes were also analysed using initially microtuber formed and non-formed plants. The results showed that for most potato genotypes, compared with other treatments, 23℃/13℃ treatment significantly prolonged the initial time of in vitrotuberization, 23℃/23℃ treatment reduced the percentage of in vitrotuberization, 23℃/18℃ treatment tuberization time was early and the average percentage was 84.3%, the most conducive to the formation of test in vitrotuberization. Potato in vitrotuberization formation was affected on night temperature through regulating gene expression of circadian clock, sugar metabolism and photoperiodic signaling pathway, such as StFKF1, StCO1and StSWEET1.

Key words: Potato, In vitro tuberization, Night temperature, Initiation time of tuberization, Gene expression

刘菊, 李广存, 段绍光, 胡军, 简银巧, 刘建刚, 金黎平, 徐建飞. 不同夜间温度处理对马铃薯试管薯及块茎形成相关基因表达的影响[J]. 作物杂志, 2022 (3): 92–98

Liu Ju, Li Guangcun, Duan Shaoguang, Hu Jun, Jian Yinqiao, Liu Jiangang, Jin Liping, Xu Jianfei. The Effects of Different Night Temperature Treatments on in vitro Tuberization and Related-Genes Expression in Potato[J]. Crops, 2022(3): 92–98

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基因名称 Gene name	基因 Gene	功能 Function	引物序列（5’→3’） Primer sequence (5’→3’)
StFKF1	PGSC0003DMT400051416	蓝光受体蛋白基因,光温信号感应	F：GCTCTACTGGTTATCGCGCT R：GGGTCACTGTGCCATCATCA
StTOC1	PGSC0003DMT400083086	生物钟基因,昼夜节律调节	F：GGCATGTGCTTTCAACCAGG R：CCTCAAACGTGGTCTCTGCT
StELF4	PGSC0003DMT400003078	生物钟基因,昼夜节律调节	F：CAGGTGCAGTCTGTGTTGGA R：TTAGGCTCACGTTCTGCACC
StCO1	PGSC0003DMT400026065	CONSTANS家族成员,块茎形成负调节因子	F：GTAGCAACAATTGGGCAAGGG R：AGTAAACGGTACATGTTGCGGA
StSP6A	PGSC0003DMG400023365	拟南芥FT同源基因,块茎形成正调节因子	F：ACAGTGTATGCCCCAGGTTG R：AACAGCTGCAACAGGCAATC
StSEX4	PGSC0003DMT400039423	蛋白络氨酸磷酸酶基因,参与淀粉降解	F：GCCGATCACTTCTCCCAACA R：TTCCGTATTGCTGCACTGGT
StSWEET1	PGSC0003DMG400024818	蔗糖转运蛋白基因,调节蔗糖流出	F：GTCTGCCATTTGTGTCACCA R：AATGCCTCAAGTCCTGCTCC
EF1α	PGSC0003DMT400059830	荧光定量内参基因	F：ATTGGAAACGGATATGCTCCA R：TCCTTACCTGAACGCCTGTCA