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作物杂志,2022, 第3期: 92–98 doi: 10.16035/j.issn.1001-7283.2022.03.013

• 遗传育种·种质资源·生物技术 • 上一篇    下一篇

不同夜间温度处理对马铃薯试管薯及块茎形成相关基因表达的影响

刘菊(), 李广存, 段绍光, 胡军, 简银巧, 刘建刚, 金黎平, 徐建飞()   

  1. 中国农业科学院蔬菜花卉研究所/农业农村部薯类作物生物学和遗传育种重点实验室,100081,北京
  • 收稿日期:2021-04-30 修回日期:2021-05-27 出版日期:2022-06-15 发布日期:2022-06-20
  • 通讯作者: 徐建飞
  • 作者简介:刘菊,主要从事环境因子影响块茎发育研究,E-mail： liuju413@163.com
  • 基金资助:
    国家自然科学基金(31771860);国家马铃薯产业技术体系项目(CARS-9)

The Effects of Different Night Temperature Treatments on in vitro Tuberization and Related-Genes Expression in Potato

Liu Ju(), Li Guangcun, Duan Shaoguang, Hu Jun, Jian Yinqiao, Liu Jiangang, Jin Liping, Xu Jianfei()   

  1. Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences/Key Laboratory of Biology and Genetic Improvement of Tuber and Root Crops, Ministry of Agriculture and Rural Affairs, Beijing 100081, China
  • Received:2021-04-30 Revised:2021-05-27 Online:2022-06-15 Published:2022-06-20
  • Contact: Xu Jianfei

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摘要：

为探究不同夜间温度处理对马铃薯试管薯形成的影响,以7个马铃薯品种或材料的试管苗为试材,在昼/夜温度分别为23℃/13℃、23℃/18℃和23℃/23℃条件下进行试管薯的诱导培养,调查记录试管薯初始结薯时间、结薯率和结薯方式变化,并分析不同夜间温度处理下试管薯初始形成植株及同期未形成试管薯植株参与块茎形成相关基因的表达量变化。结果表明,对于大多数基因型,与其他处理相比,23℃/13℃处理显著延长试管薯初始结薯时间,23℃/23℃处理降低结薯率,而23℃/18℃处理结薯较早且试管薯结薯率平均达84.3%,最利于试管薯形成。夜间温度可通过影响生物钟、糖代谢和光周期通路中StFKF1、StCO1和StSWEET1等相关基因的表达而调控块茎形成。

关键词: 马铃薯, 试管薯形成, 夜间温度, 初始结薯时间, 基因表达

Abstract:

In order to investigate the effects of different night-time temperature treatments on in vitrotuberization in potato, the initial time, percentage and type of in vitrotuberization were recorded using seven potato varieties or materials induced cultured at day/night temperature of 23℃/13℃, 23℃/18℃ and 23℃/23℃. The expression changes in tuberization-related genes were also analysed using initially microtuber formed and non-formed plants. The results showed that for most potato genotypes, compared with other treatments, 23℃/13℃ treatment significantly prolonged the initial time of in vitrotuberization, 23℃/23℃ treatment reduced the percentage of in vitrotuberization, 23℃/18℃ treatment tuberization time was early and the average percentage was 84.3%, the most conducive to the formation of test in vitrotuberization. Potato in vitrotuberization formation was affected on night temperature through regulating gene expression of circadian clock, sugar metabolism and photoperiodic signaling pathway, such as StFKF1, StCO1and StSWEET1.

Key words: Potato, In vitro tuberization, Night temperature, Initiation time of tuberization, Gene expression

图1

‘RH’试管薯结薯方式示意图 红色箭头指向试管薯,标尺=1cm

表1

实时定量PCR引物信息

基因名称
Gene name		 基因
Gene		 功能
Function		 引物序列（5’→3’）
Primer sequence (5’→3’)
StFKF1
PGSC0003DMT400051416
蓝光受体蛋白基因,光温信号感应
F：GCTCTACTGGTTATCGCGCT
R：GGGTCACTGTGCCATCATCA
StTOC1
PGSC0003DMT400083086
生物钟基因,昼夜节律调节
F：GGCATGTGCTTTCAACCAGG
R：CCTCAAACGTGGTCTCTGCT
StELF4
PGSC0003DMT400003078
生物钟基因,昼夜节律调节
F：CAGGTGCAGTCTGTGTTGGA
R：TTAGGCTCACGTTCTGCACC
StCO1
PGSC0003DMT400026065
CONSTANS家族成员,块茎形成负调节因子
F：GTAGCAACAATTGGGCAAGGG
R：AGTAAACGGTACATGTTGCGGA
StSP6A
PGSC0003DMG400023365
拟南芥FT同源基因,块茎形成正调节因子
F：ACAGTGTATGCCCCAGGTTG
R：AACAGCTGCAACAGGCAATC
StSEX4
PGSC0003DMT400039423
蛋白络氨酸磷酸酶基因,参与淀粉降解
F：GCCGATCACTTCTCCCAACA
R：TTCCGTATTGCTGCACTGGT
StSWEET1
PGSC0003DMG400024818
蔗糖转运蛋白基因,调节蔗糖流出
F：GTCTGCCATTTGTGTCACCA
R：AATGCCTCAAGTCCTGCTCC
EF1α
PGSC0003DMT400059830
荧光定量内参基因
F：ATTGGAAACGGATATGCTCCA
R：TCCTTACCTGAACGCCTGTCA

图2

不同夜间温度处理对试管薯初始结薯时间的影响 不同小写字母表示同一基因型不同夜间温度处理下在P < 0.05水平差异显著性,下同

图3

不同夜间温度处理对试管薯结薯率的影响

图4

不同夜间温度处理对试管薯结薯方式的影响

图5

不同夜间温度处理下块茎形成相关基因表达量的分析 PT：结薯植株,NPT：同一时间下的非结薯植株

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